The effect of MG132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH

Oncology Reports

Volumn 22, Issue 1, 2009, Pages 215-221

  Han, Yong Hwan ^a   Moon, Hwa Jin ^a   You, Bo Ra ^a   Park, Woo Hyun ^a  

^a Chonbuk National University   (South Korea)

Author keywords

Apoptosis; Cell cycle; Glutathione; HeLa; MG132; Reactive oxygen species

Indexed keywords

BENZYLOXYCARBONYLLEUCYLLEUCYLLEUCINAL; GLUTATHIONE PEROXIDASE; LIPOCORTIN 5; OXYGEN; REACTIVE OXYGEN METABOLITE;

APOPTOSIS; ARTICLE; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CELL GROWTH; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; FEMALE; FLOW CYTOMETRY; GROWTH INHIBITION; HELA CELL; HUMAN; HUMAN CELL; IC 50; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; PROTEIN DEPLETION; STAINING;

ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; CYSTEINE PROTEINASE INHIBITORS; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; GLUTATHIONE; HELA CELLS; HUMANS; INHIBITORY CONCENTRATION 50; LEUPEPTINS; MEMBRANE POTENTIAL, MITOCHONDRIAL; PROTEASOME ENDOPEPTIDASE COMPLEX; REACTIVE OXYGEN SPECIES; UTERINE CERVICAL NEOPLASMS;

EID: 68249153842     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or_00000427     Document Type: Article

References (39)

1. Gonzalez C, Sanz-Alfayate G, Agapito MT, Gomez-Nino A, Rocher A and Obeso A: Significance of ROS in oxygen sensing in cell systems with sensitivity to physiological hypoxia. Respir Physiol Neurobiol 132: 17-41, 2002.
2. Baran CP, Zeigler MM, Tridandapani S and Marsh CB: The role of ROS and RNS in regulating life and death of blood monocytes. Curr Pharm Des 10: 855-866, 2004.
3. Zorov DB, Juhaszova M and Sollott SJ: Mitochondrial ROS-induced ROS release: an update and review. Biochim Biophys Acta 1757: 509-517, 2006.
4. Wilcox CS: Reactive oxygen species: roles in blood pressure and kidney function. Curr Hypertens Rep 4: 160-166, 2002.
5. Poot M, Teubert H, Rabinovitch PS and Kavanagh TJ: De novo synthesis of glutathione is required for both entry into and progression through the cell cycle. J Cell Physiol 163: 555-560, 1995.
6. Schnelldorfer T, Gansauge S, Gansauge F, Schlosser S, Beger HG and Nussler AK: Glutathione depletion causes cell growth inhibition and enhanced apoptosis in pancreatic cancer cells. Cancer 89: 1440-1447, 2000.
7. Lauterburg BH: Analgesics and glutathione. Am J Ther 9: 225-233, 2002.
8. Dasmahapatra G, Rahmani M, Dent P and Grant S: The tyrphostin adaphostin interacts synergistically with proteasome inhibitors to induce apoptosis in human leukemia cells through a reactive oxygen species (ROS)-dependent mechanism. Blood 107: 232-240, 2006.
9. Wallach-Dayan SB, Izbicki G, Cohen PY, Gerstl-Golan R, Fine A and Breuer R: Bleomycin initiates apoptosis of lung epithelial cells by ROS but not by Fas/FasL pathway. Am J Physiol Lung Cell Mol Physiol 290: L790-L796, 2006.
10. Shim HY, Park JH, Paik HD, Nah SY, Kim DS and Han YS: Acacetin-induced apoptosis of human breast cancer MCF-7 cells involves caspase cascade, mitochondria-mediated death signaling and SAPK/JNK1/2-c-Jun activation. Mol Cells 24: 95-104, 2007.
11. Orlowski RZ: The role of the ubiquitin-proteasome pathway in apoptosis. Cell Death Differ 6: 303-313, 1999.
12. Voges D, Zwickl P and Baumeister W: The 26S proteasome: a molecular machine designed for controlled proteolysis. Annu Rev Biochem 68: 1015-1068, 1999.
13. Orlowski M and Wilk S: Catalytic activities of the 20 S proteasome, a multicatalytic proteinase complex. Arch Biochem Biophys 383: 1-16, 2000.
14. Adams J: The proteasome: a suitable antineoplastic target. Nat Rev Cancer 4: 349-360, 2004.
15. Drexler HC: Activation of the cell death program by inhibition of proteasome function. Proc Natl Acad Sci USA 94: 855-860, 1997.
16. Shah SA, Potter MW and Callery MP: Ubiquitin proteasome pathway: implications and advances in cancer therapy. Surg Oncol 10: 43-52, 2001.
17. Lee DH and Goldberg AL: Proteasome inhibitors: valuable new tools for cell biologists. Trends Cell Biol 8: 397-403, 1998.
18. Wu HM, Chi KH and Lin WW: Proteasome inhibitors stimulate activator protein-1 pathway via reactive oxygen species production. FEBS Lett 526: 101-105, 2002.
19. Wu HM, Wen HC and Lin WW: Proteasome inhibitors stimulate interleukin-8 expression via Ras and apoptosis signal-regulating kinase-dependent extracellular signal-related kinase and c-Jun N-terminal kinase activation. Am J Respir Cell Mol Biol 27: 234-243, 2002.
20. Ling YH, Liebes L, Zou Y and Perez-Soler R: Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic response to Bortezomib, a novel proteasome inhibitor, in human H460 non-small cell lung cancer cells. J Biol Chem 278: 33714-33723, 2003.
21. Perez-Galan P, Roue G, Villamor N, Montserrat E, Campo E and Colomer D: The proteasome inhibitor bortezomib induces apoptosis in mantle-cell lymphoma through generation of ROS and Noxa activation independent of p53 status. Blood 107: 257-264, 2006.
22. Emanuele S, Calvaruso G, Lauricella M, Giuliano M, Bellavia G, D'Anneo A, Vento R and Tesoriere G: Apoptosis induced in hepatoblastoma HepG2 cells by the proteasome inhibitor MG132 is associated with hydrogen peroxide production, expression of Bcl-XS and activation of caspase-3. Int J Oncol 21: 857-865, 2002.
23. Qiu JH, Asai A, Chi S, Saito N, Hamada H and Kirino T: Proteasome inhibitors induce cytochrome c-caspase-3-like protease-mediated apoptosis in cultured cortical neurons. J Neurosci 20: 259-265, 2000.
24. Park WH, Seol JG, Kim ES, Hyun JM, Jung CW, Lee CC, Kim BK and Lee YY: Arsenic trioxide-mediated growth inhibition in MC/CAR myeloma cells via cell cycle arrest in association with induction of cyclin-dependent kinase inhibitor, p21, and apoptosis. Cancer Res 60: 3065-3071, 2000.
25. Han YH, Kim SZ, Kim SH and Park WH: Arsenic trioxide inhibits growth of As4.1 juxtaglomerular cells via cell cycle arrest and caspase-independent apoptosis. Am J Physiol Renal Physiol 293: F511-F520, 2007.
26. Setsukinai K, Urano Y, Kakinuma K, Majima HJ and Nagano T: Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. J Biol Chem 278: 3170-3175, 2003.
27. Han YH, Kim SH, Kim SZ and Park WH: Caspase inhibitor decreases apoptosis in pyrogallol-treated lung cancer Calu-6 cells via the prevention of GSH depletion. Int J Oncol 33: 1099-1105, 2008.
28. Macho A, Hirsch T, Marzo I, Marchetti P, Dallaporta B, Susin SA, Zamzami N and Kroemer G: Glutathione depletion is an early and calcium elevation is a late event of thymocyte apoptosis. J Immunol 158: 4612-4619, 1997.
29. Hedley DW and Chow S: Evaluation of methods for measuring cellular glutathione content using flow cytometry. Cytometry 15: 349-358, 1994.
30. Kastan MB and Bartek J: Cell-cycle checkpoints and cancer. Nature 432: 316-323, 2004.
31. Wu WK, Wu YC, Yu L, Li ZJ, Sung JJ and Cho CH: Induction of autophagy by proteasome inhibitor is associated with proliferative arrest in colon cancer cells. Biochem Biophys Res Commun 374: 258-263, 2008.
32. Yan XB, Yang DS, Gao X, Feng J, Shi ZL and Ye Z: Caspase-8 dependent osteosarcoma cell apoptosis induced by proteasome inhibitor MG132. Cell Biol Int 31: 1136-1143, 2007.
33. Zhao P, Zhong W, Ying X, Yuan Z, Fu J and Zhou Z: Manganese chloride-induced G0/G1 and S phase arrest in A549 cells. Toxicology 250: 39-46, 2008.
34. Zhang W, Tong Q, Li S, Wang X and Wang Q: MG-132 inhibits telomerase activity, induces apoptosis and G(1) arrest associated with upregulated p27kip1 expression and downregulated survivin expression in gastric carcinoma cells. Cancer Invest 26: 1032-1036, 2008.
35. Yang J, Liu X, Bhalla K, Kim CN, Ibrado AM, Cai J, Peng TI, Jones DP and Wang X: Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science 275: 1129-1132, 1997.
36. Estrela JM, Ortega A and Obrador E: Glutathione in cancer biology and therapy. Crit Rev Clin Lab Sci 43: 143-181, 2006.
37. Higuchi Y: Glutathione depletion-induced chromosomal DNA fragmentation associated with apoptosis and necrosis. J Cell Mol Med 8: 455-464, 2004.
38. Park WH, Han YW, Kim SH and Kim SZ: A superoxide anion generator, pyrogallol induces apoptosis in As4.1 cells through the depletion of intracellular GSH content. Mutat Res 619: 81-92, 2007.
39. Jahngen-Hodge J, Obin MS, Gong X, Shang F, Nowell TR Jr, Gong J, Abasi H, Blumberg J and Taylor A: Regulation of ubiquitin-conjugating enzymes by glutathione following oxidative stress. J Biol Chem 272: 28218-28226, 1997.