Targeting expression of the catalytic domain of the kinase insert domain receptor (KDR) in the peroxisomes of Pichia pastoris

FEMS Yeast Research

Volumn 9, Issue 5, 2009, Pages 732-741

  Wang, Ya ^a   Xuan, Yaoji ^a   Zhang, Ping ^a   Jiang, Xi ^a   Ni, Zhenhua ^a   Tong, Linjiang ^a   Zhou, Xiangshan ^a   Lin, Liping ^a   Ding, Jian ^b   Zhang, Yuanxing ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

KDR; Kinase insert domain receptor; Peroxisome; Phosphorylation; Pichia pastoris; Vascular endothelial growth factor receptor 2

Indexed keywords

GREEN FLUORESCENT PROTEIN; HISTIDINE; HYBRID PROTEIN; PROTEIN SKL; UNCLASSIFIED DRUG; VASCULOTROPIN RECEPTOR 2;

AMINO TERMINAL SEQUENCE; AOX1 GENE; ARTICLE; CELL GROWTH; CLONE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME MECHANISM; FLOW CYTOMETRY; FLUORESCENCE; FUNGAL GENE; GENE EXPRESSION; GENE TARGETING; HOST CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PEROXISOME; PICHIA PASTORIS; PROMOTER REGION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION;

CATALYTIC DOMAIN; GENES, REPORTER; LUMINESCENT PROTEINS; PEROXISOMES; PICHIA; RECOMBINANT FUSION PROTEINS; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2;

PICHIA PASTORIS;

EID: 67651171556     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2009.00521.x     Document Type: Article

References (44)

1. Aksam EB, Koek A, Kiel JA, Jourdan S, Veenhuis M van der Klei IJ (2007) A peroxisomal lon protease and peroxisome degradation by autophagy play key roles in vitality of Hansenula polymorpha cells. Autophagy 3 : 96 105.
2. Beebe JS, Jani JP, Knauth E et al 2003) Pharmacological characterization of CP-547,632, a novel vascular endothelial growth factor receptor-2 tyrosine kinase inhibitor for cancer therapy. Cancer Res 63 : 7301 7309.
3. Brignola PS, Lackey K, Kadwell SH et al 2002) Comparison of the biochemical and kinetic properties of the type 1 receptor tyrosine kinase intracellular domains. Demonstration of differential sensitivity to kinase inhibitors. J Biol Chem 277 : 1576 1585.
4. Cereghino JL Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24 : 45 66.
5. Cormack BP, Bertram G, Egerton M, Gow NA, Falkow S Brown AJ (1997) Yeast-enhanced green fluorescent protein (yEGFP)a reporter of gene expression in Candida albicans. Microbiology 143 : 303 311.
6. Distel B, Gould SJ, Voorn-Brouwer T, van der Berg M, Tabak HF Subramani S (1992) The carboxyl-terminal tripeptide serine-lysine-leucine of firefly luciferase is necessary but not sufficient for peroxisomal import in yeast. New Biol 4 : 157 165.
7. Dougher-Vermazen M, Hulmes JD, Böhlen P Terman BI (1994) Biological activity and phosphorylation sites of the bacterially expressed cytosolic domain of the KDR VEGF-receptor. Biochem Bioph Res Co 205 : 728 738.
8. Faber KN, Westra S, Waterham HR, Keizer-Gunnink I, Harder W, AB G Veenhuis M (1996) Foreign gene expression in Hansenula polymorpha. A system for the synthesis of small functional peptides. Appl Microbiol Biot 45 : 72 79.
9. Farre JC, Shirahama-Noda K, Zhang L, Booher K Subramani S (2007) Localization of proteins and organelles using fluorescence microscopy. Meth Mol Biol 389 : 239 250.
10. Ferrara N, Gerber HP LeCouter J (2003) The biology of VEGF and its receptors. Nat Med 9 : 669 676.
11. Folkman J (1995) Angiogenesis in cancer, vascular, rheumatoid, and other disease. Nat Med 1 : 27 31.
12. Gidijala L, van der Klei IJ, Veenhuis M Kiel JA (2007) Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene. FEMS Yeast Res 7 : 1160 1167.
13. Gidijala L, Bovenberg RA, Klaassen P, van der Klei IJ, Veenhuis M Kiel JA (2008) Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha. BMC Biotechnol 8 : 29.
14. Gould SJ, McCollum D, Spong AP, Heyman JA Subramani S (1992) Development of the yeast Pichia pastoris as a model organism for a genetic and molecular analysis of peroxisome assembly. Yeast 8 : 613 628.
15. Harder W Veenhuis M (1989) Metabolism of one-carbon compounds. The Yeasts, Vol. 3, 2nd edn (Rose AH Harrison JS, eds), pp. 289 316. Academic Press, London.
16. Hohenblum H, Borth N Mattanovich D (2003) Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J Biotechnol 102 : 281 290.
17. Hubbard SR Miller WT (2007) Receptor tyrosine kinases: mechanisms of activation and signaling. Curr Opin Cell Biol 19 : 117 123.
18. Johnson MA, Waterham HR, Ksheminska GP, Fayura LR, Cereghino JL, Stasyk OV, Veenhuis M, Kulachkovsky AR, Sibirny AA Cregg JM (1999) Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris. Genetics 151 : 1379 1391.
19. Lam LP, Chow RY Berger SA (1999) A transforming mutation enhances the activity of the c-Kit soluble tyrosine kinase domain. Biochem J 338 : 131 138.
20. Liu C, Guo L, Yao C, Zhang R Li Y (2008) Expression and purification of human vascular-endothelial-growth-factor-receptor-2 tyrosine kinase in Streptomyces for inhibitor screening. Biotechnol Appl Bioc 50 : 113 119.
21. Lutz MV, Bovenberg RA, van der Klei IJ Veenhuis M (2005) Synthesis of Penicillium chrysogenum acetyl-CoA: isopenicillin N-acyltransferase in Hansenula polymorpha: first step towards the introduction of a new metabolic pathway. FEMS Yeast Res 5 : 1063 1067.
22. Mendel DB, Laird AD, Xin X et al 2003) In vivo antitumor activity of SU11248, a novel tyrosine kinase inhibitor targeting vascular endothelial growth factor and platelet-derived growth factor receptors: determination of a pharmacokinetic/pharmacodynamic relationship. Clin Cancer Res 9 : 327 337.
23. Moasser MM, Basso A, Averbuch SD Rosen N (2001) The tyrosine kinase inhibitor ZD1839 ('Iressa') inhibits HER2-driven signaling and suppresses the growth of HER2-overexpressing tumor cells. Cancer Res 61 : 7184 7188.
24. Monosov EZ, Wenzel TJ, Lüers GH, Heyman JA Subramani S (1996) Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells. J Histochem Cytochem 44 : 581 589.
25. Oh DB, Park JS, Kim MW, Cheon SA, Kim EJ, Moon HY, Kwon O, Rhee SK Kang HA (2008) Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly. Biotechnol J 3 : 659 668.
26. Parast CV, Mroczkowski B, Pinko C, Misialek S, Khambatta G Appelt K (1998) Characterization and kinetic mechanism of catalytic domain of human vascular endothelial growth factor receptor-2 tyrosine kinase (VEGFR2 TK), a key enzyme in angiogenesis. Biochemistry 37 : 16788 16801.
27. Song SM Shou CC (1998) Molecular cloning of vascular endothelial growth factor receptor (KDR) and its prokaryotic expression. Chin J Biochem Mol Biol 14 : 57 61.
28. Superti-Furga G, Jönsson K Courtneidge SA (1996) A functional screen in yeast for regulators and antagonizers of heterologous protein tyrosine kinases. Nat Biotechnol 14 : 600 605. (Pubitemid 26135123)
29. Underiner TL, Ruggeri B Gingrich DE (2004) Development of vascular endothelial growth factor receptor (VEGFR) kinase inhibitors as anti-angiogenic agents in cancer therapy. Curr Med Chem 11 : 731 745.
30. van der Klei I Veenhuis M (2007) Protein targeting to yeast peroxisomes. Meth Mol Biol 390 : 373 392.
31. van der Klei IJ, Yurimoto H, Sakai Y Veenhuis M (2006) The significance of peroxisomes in methanol metabolism in methylotrophic yeast. Biochim Biophys Acta 1763 : 1453 1462.
32. van Dijk R, Faber KN, Kiel JA, Veenhuis M van der Klei I (2000) The methylotrophic yeast Hansenula polymorpha: a versatile cell factory. Enzyme Microb Technol 26 : 793 800.
33. Veenhuis M, van Dijken JP Harder W (1983) The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts. Adv Microb Physiol 24 : 1 82.
34. Veeravagu A, Hsu AR, Cai W, Hou LC, Tse VC Chen X (2007) Vascular endothelial growth factor and vascular endothelial growth factor receptor inhibitors as anti-angiogenic agents in cancer therapy. Recent Patents Anticancer Drug Discov 2 : 59 71.
35. Walkenhorst J, Goga A, Witte ON Superti-Furga G (1996) Analysis of human c-Abl tyrosine kinase activity and regulation in S. pombe. Oncogene 12 : 1513 1520.
36. Waterham HR, Russell KA, de Vries Y Cregg JM (1997) Peroxisomal targeting, import, and assembly of alcohol oxidase in Pichia pastores. J Cell Biol 139 : 1419 1431.
37. Wei C, Zhou XS Zhang YX (2008) Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme. Appl Microbiol Biot 78 : 257 264.
38. Wu JM, Hsu TA Lee CK (2003) Expression of the gene coding for bacterial hemoglobin improves beta-galactosidase production in a recombinant Pichia pastoris. Biotechnol Lett 25 : 1457 1462.
39. Xiao AF, Zhou XS, Zhou L Zhang YX (2006) Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation. 72 : 837 844.
40. Zeng GF, Zhang ZQ, Zhang LG, Chen AJ, Yao LH Hou YD (2001) Cloning of VEGF receptor KDR and its expression in insect cells. Sheng Wu Gong Cheng Xue Bao 17 : 140 144.
41. Zhao G, Peery RB Yingling JM (2007) Characterization and development of a peptide substrate-based phosphate transfer assay for the human vascular endothelial growth factor receptor-2 tyrosine kinase. Anal Biochem 360 : 196 206.
42. Zhong L, Guo XN, Zhang XH, Wu ZX, Luo XM, Jiang HL, Lin LP, Zhang XW Ding J (2005) Expression and purification of the catalytic domain of human vascular endothelial growth factor receptor 2 for inhibitor screening. Biochim Biophys Acta 1722 : 254 261.
43. Zhou XS, Lu J, Fan WM Zhang YX (2002) Development of a responsive methanol sensor and its application in Pichia pastoris fermentation. Biotechnol Lett 24 : 643 646.
44. Zuo Q, Tian L, Hou JM, Wang YS, Wen YJ, Li J Wei YQ (2006) Expression of extracellular domain of human vascular endothelial growth factor receptor-2 in Pichia pastoris. Sichuan Da Xue Xue Bao Yi Xue Ban 37 : 1 4.