Localization of proteins and organelles using fluorescence microscopy

Methods in Molecular Biology

Volumn 389, Issue , 2007, Pages 239-249

  Farre, Jean Claude ^a   Shirahama Noda, Kanae ^a   Zhang, Lan ^a   Booher, Keith ^a   Subramani, Suresh ^a  

^a NONE

Author keywords

Fluorescence microscopy; Green fluorescent protein variants; Organelle markers; Peroxisomes; Protein colocalization; Protein localization

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; INDOLE DERIVATIVE; PROTEIN;

ARTICLE; CELL NUCLEUS; CELL ORGANELLE; CELL VACUOLE; CYTOLOGY; FLUORESCENCE MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; METABOLISM; MICROBIAL VIABILITY; MITOCHONDRION; PICHIA; PROTEIN TRANSPORT;

CELL NUCLEUS; FLUORESCENT ANTIBODY TECHNIQUE; INDOLES; MICROBIAL VIABILITY; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; ORGANELLES; PICHIA; PROTEIN TRANSPORT; PROTEINS; VACUOLES;

PICHIA PASTORIS;

EID: 36549020483     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-456-7:239     Document Type: Article

References (8)

1. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887.
2. Rossanese, O. W., Soderholm, J., Bevis, B. J., et al. (1999) Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae. J. Cell Biol. 145, 69-81.
3. Vida, T. A. and Emr, S. D. (1995) A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J. Cell Biol. 128, 779-792.
4. Dann, O., Bergen, G., Demant, E., and Voltz, G. (1971) Trypanocide Diamidine des 2-Phenyl-benofurans, 2-Phenyl-indens und 2-Phenyl-indols. Liebigs Ann. Chemi. 749, 68-89.
5. Gould, S. J., Keller, G. A., and Subramani, S. (1987) Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase. J. Cell Biol. 105, 2923-2931.
6. Campbell, R. E., Tour, O., Palmer, A. E., et al. (2002) A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 7877-7882.
7. Wiemer, E. A., Luers, G. H., Faber, K. N., Wenzel, T., Veenhuis, M., and Subramani, S. (1996) Isolation and characterization of Pas2p, a peroxisomal membrane protein essential for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris. J Biol. Chem. 271, 18,973-18,980.
8. Sears, I. B., O'Connor, J., Rossanese, O. W., and Glick, B. S. (1998) A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Yeast 14, 783-790.