Multilayer mediated forward and patterned siRNA transfection using linear-PEI at extended N/P ratios

Acta Biomaterialia

Volumn 5, Issue 5, 2009, Pages 1474-1488

  Mehrotra, Sumit ^a   Lee, Ilsoon ^a   Chan, Christina ^a,b  

^a Michigan State University   (United States)

^b Michigan State University   (United States)

Author keywords

Layer by layer (LbL) assembly; LPEI siRNA nanoparticles; N P ratio; Patterned transfection; siRNA transfection

Indexed keywords

EFFICIENCY; GENE TRANSFER; MOLECULAR BIOLOGY; MULTILAYERS; NUCLEIC ACIDS;

LAYER-BY-LAYER ASSEMBLIES; LINEAR POLYETHYLENIMINE; LINEAR POLYETHYLENIMINE-SMALL INTERFERING RNA NANOPARTICLE; N:P RATIO; PATTERNED TRANSFECTION; SMALL INTERFERING RNA; SMALL INTERFERING RNA TRANSFECTION; TRANSFECTION EFFICIENCY; TRANSFECTION REAGENTS;

NANOPARTICLES;

NANOPARTICLE; NITROGEN; PHOSPHATE; POLYETHYLENEIMINE; SMALL INTERFERING RNA;

ACCURACY; ARTICLE; CELL ADHESION; CELL SURFACE; COMPLEX FORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; LIGHT; PARTICLE SIZE; PH; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RNA INTERFERENCE; ULTRAVIOLET RADIATION;

EID: 67349175996     PISSN: 17427061     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actbio.2009.01.004     Document Type: Article

References (52)

1. Fire A., Xu S.Q., Montgomery M.K., Kostas S.A., Driver S.E., and Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391 (1998) 806-811
2. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
3. Cheema S.K., Chen E., Shea L.D., and Mathur A.B. Regulation and guidance of cell behavior for tissue regeneration via the siRNA mechanism. Wound Repair Regen 15 (2007) 286-295
4. Ryther R.C.C., Flynt A.S., Phillips J.A., and Patton J.G. SiRNA therapeutics: big potential from small RNAs. Gene Ther 12 (2005) 5-11
5. Bonadio J., Smiley E., Patil P., and Goldstein S. Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nat Med 5 (1999) 753-759
6. De Laporte L., and Shea L.D. Matrices and scaffolds for DNA delivery in tissue engineering. Adv Drug Deliv Rev 59 (2007) 292-307
7. Houchin-Ray T., Whittlesey K.J., and Shea L.D. Spatially patterned gene delivery for localized neuron survival and neurite extension. Mol Ther 15 (2007) 705-712
8. Pannier A.K., Anderson B.C., and Shea L.D. Substrate-mediated delivery from self-assembled monolayers: effect of surface ionization, hydrophilicity, and patterning. Acta Biomater 1 (2005) 511-522
9. Wheeler D.B., Carpenter A.E., and Sabatini D.M. Cell microarrays and RNA interference chip away at gene function. Nat Genet 37 (2005) S25-S30
10. Ziauddin J., and Sabatini D.M. Microarrays of cells expressing defined cDNAs. Nature 411 (2001) 107-110
11. Diaz-Mochon J.J., Tourniaire G., and Bradley M. Microarray platforms for enzymatic and cell-based assays. Chem Soc Rev 36 (2007) 449-457
12. Kong H.J., Liu J.D., Riddle K., Matsumoto T., Leach K., and Mooney D.J. Non-viral gene delivery regulated by stiffness of cell adhesion substrates. Nat Mater 4 (2005) 460-464
13. Decher G. Fuzzy nanoassemblies: toward layered polymeric multicomposites. Science 277 (1997) 1232-1237
14. Decher G., Hong J.D., and Schmitt J. Buildup of ultrathin multilayer films by a self-assembly process. 3. Consecutively alternating adsorption of anionic and cationic polyelectrolytes on charged surfaces. Thin Solid Films 210 (1992) 831-835
15. Lynn D.M. Peeling back the layers: Controlled erosion and triggered disassembly of multilayered polyelectrolyte thin films. Adv Mater 19 (2007) 4118-4130
16. Tang Z.Y., Wang Y., Podsiadlo P., and Kotov N.A. Biomedical applications of layer-by-layer assembly: from biomimetics to tissue engineering. Adv Mater 18 (2006) 3203-3224
17. Kumar A., and Whitesides G.M. Features of gold having micrometer to centimeter dimensions can be formed through a combination of stamping with an elastomeric stamp and an alkanethiol ink followed by chemical etching. Appl Phys Lett 63 (1993) 2002-2004
18. Xia Y.N., and Whitesides G.M. Soft lithography. Angew Chem Int Ed 37 (1998) 551-575
19. Xia Y. Soft lithography and the art of patterning - a tribute to Professor George M. Whitesides. Adv Mater 16 (2004) 1245-1246
20. Ruiz S.A., and Chen C.S. Microcontact printing: a tool to pattern. Soft Matter 3 (2007) 168-177
21. Jewell C.M., Zhang J.T., Fredin N.J., and Lynn D.M. Multilayered polyelectrolyte films promote the direct and localized delivery of DNA to cells. J Control Release 106 (2005) 214-223
22. Yamauchi F., Kato K., and Iwata H. Layer-by-layer assembly of poly(ethyleneimine) and plasmid DNA onto transparent indium-tin oxide electrodes for temporally and spatially specific gene transfer. Langmuir 21 (2005) 8360-8367
23. Gary D.J., Puri N., and Won Y.Y. Polymer-based siRNA delivery: perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery. J Control Release 121 (2007) 64-73
24. Jessel N., Oulad-Abdeighani M., Meyer F., Lavalle P., Haikel Y., Schaaf P., et al. Multiple and time-scheduled in situ DNA delivery mediated by beta-cyclodextrin embedded in a polyelectrolyte multilayer. Proc Natl Acad Sci USA 103 (2006) 8618-8621
25. Meyer F., Ball V., Schaaf P., Voegel J.C., and Ogier J. Polyplex-embedding in polyelectrolyte multilayers for gene delivery. Biochim Biophys Acta Biomembr 1758 (2006) 419-422
26. Ono S.S., and Decher G. Preparation of ultrathin self-standing polyelectrolyte multilayer membranes at physiological conditions using pH-responsive film segments as sacrificial layers. Nano Lett 6 (2006) 592-598
27. Neu M., Fischer D., and Kissel T. Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives. J Gene Med 7 (2005) 992-1009
28. Merdan T., Kopecek J., and Kissel T. Prospects for cationic polymers in gene and oligonucleotide therapy against cancer. Adv Drug Deliv Rev 54 (2002) 715-758
29. Spagnou S., Miller A.D., and Keller M. Lipidic carriers of siRNA: differences in the formulation, cellular uptake, and delivery with plasmid DNA. Biochemistry 43 (2004) 13348-13356
30. Abdallah B., Hassan A., Benoist C., Goula D., Behr J.P., and Demeneix B.A. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: polyethylenimine. Hum Gene Ther 7 (1996) 1947-1954
31. Boussif O., Lezoualch F., Zanta M.A., Mergny M.D., Scherman D., Demeneix B., et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in-vivo - polyethylenimine. Proc Natl Acad Sci USA 92 (1995) 7297-7301
32. Choosakoonkriang S., Lobo B.A., Koe G.S., Koe J.G., and Middaugh C.R. Biophysical characterization of PEI/DNA complexes. J Pharm Sci 92 (2003) 1710-1722
33. Grayson A.C.R., Doody A.M., and Putnam D. Biophysical and structural characterization of polyethylenimine-mediated siRNA delivery in vitro. Pharm Res 23 (2006) 1868-1876
34. Grzelinski M., Urban-Klein B., Martens T., Lamszus K., Bakowsky U., Hobel S., et al. RNA interference-mediated gene silencing of pleiotrophin through polyethylenimine-complexed small interfering RNAs in vivo exerts antitumoral effects in glioblastoma xenografts. Hum Gene Ther 17 (2006) 751-766
35. Kunath K., von Harpe A., Fischer D., Peterson H., Bickel U., Voigt K., et al. Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine. J Control Release 89 (2003) 113-125
36. Ogris M., Steinlein P., Kursa M., Mechtler K., Kircheis R., and Wagner E. The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther 5 (1998) 1425-1433
37. Thomas M., Lu J.J., Ge Q., Zhang C.C., Chen J.Z., and Klibanov A.M. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci USA 102 (2005) 5679-5684
38. Urban-Klein B., Werth S., Abuharbeid S., Czubayko F., and Aigner A. RNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo. Gene Ther 12 (2005) 461-466
39. Shim M.S., and Kwon Y.J. Controlled delivery of plasmid DNA and siRNA to intracellular targets using ketalized polyethylenimine. Biomacromolecules 9 (2008) 444-455
40. Mendelsohn J.D., Yang S.Y., Hiller J., Hochbaum A.I., and Rubner M.F. Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films. Biomacromolecules 4 (2003) 96-106
41. Ogris M., Brunner S., Schuller S., Kircheis R., and Wagner E. PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther 6 (1999) 595-605
42. Bishop N.E. An update on non-clathrin-coated endocytosis. Rev Med Virol 7 (1997) 199-209
43. Pataer A., Vorburger S.A., Chada S., Balachandran S., Barber G.N., Roth J.A., et al. Melanoma differentiation-associated gene-7 protein physically associates with the double-stranded RNA-activated protein kinase PKR. Mol Ther 11 (2005) 717-723
44. Dalby B., Cates S., Harris A., Ohki E.C., Tilkins M.L., Price P.J., et al. Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications. Methods 33 (2004) 95-103
45. Perrine T.D., and Landis W.R. Analysis of polyethylenimine by spectrophotometry of its copper chelate. J Polym Sci Part A Polym Chem 5 (1967) 1993-2003
46. Bertschinger M., Chaboche S., Jordan M., and Wurm F.M. A spectrophotometric assay for the quantification of polyethylenimine in DNA nanoparticles. Anal Biochem 334 (2004) 196-198
47. Link S., Wang Z.L., and El-Sayed M.A. Alloy formation of gold-silver nanoparticles and the dependence of the plasmon absorption on their composition. J Phys Chem B 103 (1999) 3529-3533
48. Mallik K., Mandal M., Pradhan N., and Pal T. Seed mediated formation of bimetallic nanoparticles by UV irradiation: a photochemical approach for the preparation of "core-shell" type structures. Nano Lett 1 (2001) 319-322
49. Chen H.M., Liu R.S., Jang L.Y., Lee J.F., and Hu S.F. Characterization of core-shell type and alloy Ag/Au bimetallic clusters by using extended X-ray absorption fine structure spectroscopy. Chem Phys Lett 421 (2006) 118-123
50. Koyama Y., Yamashita M., Iida-Tanaka N., and Ito T. Enhancement of transcriptional activity of DNA complexes by amphoteric PEG derivative. Biomacromolecules 7 (2006) 1274-1279
51. Pannier A.K., Wieland J.A., and Shea L.D. Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes. Acta Biomater 4 (2008) 26-39
52. Mao S.R., Neu M., Germershaus O., Merkel O., Sitterberg J., Bakowsky U., et al. Influence of polyethylene glycol chain length on the physicochemical and biological properties of poly(ethylene imine)-graft-poly(ethylene glycol) block copolymer/SiRNA polyplexes. Bioconjug Chem 17 (2006) 1209-1218