Influence of lyophilization, fluidized bed drying, addition of protectants, and storage on the viability of lactic acid bacteria

Journal of Applied Microbiology

Volumn 107, Issue 1, 2009, Pages 167-177

  Strasser, S ^a   Neureiter, M ^a   Geppl, M ^a   Braun, R ^a   Danner, H ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Fluidized bed drying; Industrial starter cultures; Lactic acid bacteria; Lyophilization; Protectants; Shelf life

Indexed keywords

BACILLI; DRYING; ENERGY UTILIZATION; FLUIDIZED BEDS; SUSPENSIONS (FLUIDS);

DRYING TECHNOLOGY; FLUIDIZED BED DRYING; INDUSTRIAL STARTER CULTURE; LACTIC ACID BACTERIA; LACTOBACILLUS PLANTARUM; LYOPHILIZATION; PROTECTANT; SHELF LIFE; STARTER CULTURES; SURVIVAL RATE;

LACTIC ACID;

GLUCOSE; MALTODEXTRIN; PROTECTIVE AGENT; SUCROSE; TREHALOSE;

BACTERIUM; INCUBATION; POPULATION VIABILITY ANALYSIS; STORAGE; SURVIVAL;

ARTICLE; BACTERIAL VIABILITY; CONTROLLED STUDY; ENTEROCOCCUS FAECIUM; FLUIDIZED BED; FREEZE DRYING; INTERMETHOD COMPARISON; LACTOBACILLUS PLANTARUM; NONHUMAN; STORAGE; TEMPERATURE SENSITIVITY;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECIUM; LACTOBACILLUS PLANTARUM;

EID: 66849093562     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04192.x     Document Type: Article

References (51)

1. Abee, T. Wouters, J.A. (1999) Microbial stress response in minimal processing. Int J Food Microbiol 50, 65 91. (Pubitemid 29376433)
2. Baati, L., Fabre-Gea, C., Auriol, D. Blanc, P.J. (2000) Study of the cryotolerance of Lactobacillus acidophilus: effect of culture and freezing conditions on the viability and cellular protein levels. Int J Food Microbiol 59, 241 247. (Pubitemid 30628331)
3. Barbosa-Canovas, G.V. Uliano, P.J. (2004) Adaptation of classical processes to new technical developments and quality requirements. J Food Sci 69, E240 E250.
4. Bhandari, B.R. Howes, T. (1999) Implication of glass transition for the drying and stability of dried foods. J Food Eng 40, 71 79.
5. Bucio, A., Hartemink, R., Schrama, J.W., Verreth, J. Rombouts, F.M. (2005) Survival of Lactobacillus plantarum 44a after spraying and drying in feed and during exposure to gastrointestinal tract fluids in vitro. J Gen Appl Microbiol 51, 221 227. (Pubitemid 41574030)
6. Carcoba, R. Rodriguez, A. (2000) Influence of cryoprotectants on the viability and acidifying activity of frozen and freeze-dried cells of the novel starter strain Lactococcus lactis ssp. lactis CECT 5180. Eur Food Res Technol 211, 433 437.
7. Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X. Gibbs, P. (2002) Survival of freeze-dried Lactobacillus plantarum and Lactobacillus rhamnosus during storage in the presence of protectants. Biotechnol Lett 24, 1587 1591. (Pubitemid 35288800)
8. Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X. Gibbs, P. (2004a) Effects of various sugars added to growth and drying media upon thermotolerance and survival throughout storage of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus. Biotechnol Prog 20, 248 254. (Pubitemid 38204195)
9. Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X. Gibbs, P. (2004b) Relevant factors for the preparation of freeze-dried lactic acid bacteria. Int Dairy J 14, 835 847. (Pubitemid 39081551)
10. Castro, H.P., Teixeira, P.M. Kirby, R. (1995) Storage of lyophilized cultures of Lactobacillus bulgaricus under different relative humidities and atmospheres. Appl Microbiol Biotechnol 44, 172 176.
11. Champagne, C.P., Mondou, F., Raymond, Y. Roy, D. (1996) Effect of polymers and storage temperature on the stability of freeze-dried lactic acid bacteria. Food Res Int 29, 555 562. (Pubitemid 126335997)
12. Chen, T., Fowler, A. Toner, M. (2000) Literature review: supplemented phase diagram of the trehalose-water binary mixture. Cryobiology 40, 277 282. (Pubitemid 30354571)
13. Chua, K.J. Chou, S.K. (2003) Low-cost drying methods for developing countries. Trends Food Sci Technol 14, 519 528. (Pubitemid 37399476)
14. Conrad, P.B., Miller, D.P., Cielenski, P.R. de Pablo, J.J. (2000) Stabilization and preservation of Lactobacillus acidophilus in saccharide matrices. Cryobiology 41, 17 24.
15. Corcoran, B.M., Ross, R.P., Fitzgerald, G.F. Stanton, C. (2004) Comparative survival of probiotic Lactobacilli spray-dried in the presence of prebiotic substances. J Appl Microbiol 96, 1024 1039. (Pubitemid 38582149)
16. Corcoran, B.M., Ross, R.P., Fitzgerald, G.F., Dockery, P. Stanton, C. (2006) Enhanced survival of GroESL-overproducing Lactobacillus paracasei NFBC 338 under stressful conditions induced by drying. Appl Environ Microbiol 72, 5104 5107. (Pubitemid 44078849)
17. Crowe, J.H., Hoekstra, F.A. Crowe, L.M. (1992) Anhydrobiosis Annu Rev Physiol 54, 579 599.
18. De Giulio, B., Orlando, P., Barba, G., Coppola, R., Rosa, M.D., Sada, A., Prisco, P.P.D. Nazzaro, F. (2005) Use of alginate and cryo-protective sugars to improve the viability of lactic acid bacteria after freezing and freeze-drying. World J Microb Biot 21, 739 746. (Pubitemid 41073917)
19. Duong, T., Barrangou, R., Russell, W.M. Klaenhammer, T.R. (2006) Characterization of the tre locus and analysis of trehalose cryoprotection in Lactobacillus acidophilus NCFM. Appl Environ Microbiol 72, 1218 1225. (Pubitemid 43271229)
20. Efiuvwevwere, B.J.O., Gorris, L.G.M., Smid, E.J. Kets, E.P.W. (1999) Mannitol-enhanced survival of Lactococcus lactis subjected to drying. Appl Microbiol Biotechnol 51, 100 104. (Pubitemid 29066653)
21. Ferreira, V., Soares, V., Santos, C., Silva, J., Gibbs, P.A. Teixeira, P. (2005) Survival of Lactobacillus sakei during heating, drying and storage in the dried state when growth has occurred in the presence of sucrose or monosodium glutamate. Biotechnol Lett 27, 249 252. (Pubitemid 40374626)
22. Fonseca, F., Beal, C. Corrieu, G. (2000) Method of quantifying the loss of acidification activity of lactic acid starters during freezing and frozen storage. J Dairy Res 67, 83 90. (Pubitemid 30104849)
23. Fonseca, F., Passot, S., Lieben, P. Marin, M. (2004) Collapse temperature of bacterial suspensions: the effect of cell type and concentration. Cryoletters 25, 425 434. (Pubitemid 40059602)
24. Gardiner, G.E., O'Sullivan, E., Kelly, J., Auty, M.A.E., Fitzgerald, G.F., Collins, J.K., Ross, R.P. Stanton, C. (2000) Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L. salivarius strains during heat treatment and spray drying. Appl Environ Microbiol 66, 2605 2612. (Pubitemid 30353971)
25. Gomes, A.M.P. Malcata, F.X. (1999) Bifidobacterium spp. and Lactobacillus acidophilus: biological, biochemical, technological and therapeutical properties relevant for use as probiotics. Trends Food Sci Technol 10, 139 157. (Pubitemid 29524023)
26. Hoekstra, F.A., Wolkers, W.F., Buitink, J., Golovina, E.A., Crowe, J.H. Crowe, L.M. (1997) Membrane stabilization in the dry state. Comp Biochem Phys A 117, 335 341. (Pubitemid 27236696)
27. Johnson, J.A.C. Etzel, M.R. (1995) Properties of Lactobacillus helveticus CNRZ-32 attenuated by spray-drying, freeze-drying, or freezing. J Dairy Sci 78, 761 768.
28. Kim, W.S., Perl, L., Park, J.H., Tandianus, J.E. Dunn, N.W. (2001) Assessment of stress response of the probiotic Lactobacillus acidophilus. Curr Microbiol 43, 346 350. (Pubitemid 32973286)
29. Knorr, D. (1998) Technology aspects related to microorganisms in functional foods. Trends Food Sci Technol 9, 295 306. (Pubitemid 29032224)
30. Leslie, S.B., Israeli, E., Lighthart, B., Crowe, J.H. Crowe, L.M. (1995) Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying. Appl Environ Microbiol 61, 3592 3597.
31. Linders, L.J.M., de Jong, G.I.W., Meerdink, G. van't Riet, K. (1997a) Carbohydrates and the dehydration inactivation of Lactobacillus plantarum: the role of moisture distribution and water activity. J Food Eng 31, 237 250. (Pubitemid 127418062)
32. Linders, L.J.M., Wolkers, W.F., Hoekstra, F.A. van't Riet, K. (1997b) Effect of added carbohydrates on membrane phase behavior and survival of dried Lactobacillus plantarum. Cryobiology 35, 31 40. (Pubitemid 127434058)
33. Linders, L.J.M., Meerdink, G. van't Riet, K. (1997c) Effect of growth parameters on the residual activity of Lactobacillus plantarum after drying. J Appl Microbiol 82, 683 688. (Pubitemid 27283409)
34. Mille, Y., Obert, J.P., Beney, L. Gervais, P. (2004) New drying process for lactic bacteria based on their dehydration behavior in liquid medium. Biotechnol Bioeng 88, 71 76. (Pubitemid 39388695)
35. Morgan, C.A., Herman, N., White, P.A. Vesey, G. (2006) Preservation of micro-organisms by drying; A review. J Microbiol Meth 66, 183 193. (Pubitemid 43903039)
36. Palmfeldt, J. Hahn-Hagerdal, B. (2000) Influence of culture pH on survival of Lactobacillus reuteri subjected to freeze-drying. Int J Food Microbiol 55, 235 238. (Pubitemid 30160836)
37. Potts, M. (2001) Desiccation tolerance: a simple process? Trends Microbiol 9, 553 559. (Pubitemid 34121942)
38. Ratti, C. (2001) Hot air and freeze-drying of high-value foods: a review. J Food Eng 49, 311 319. (Pubitemid 32518200)
39. Saarela, M., Rantala, M., Hallamaa, K., Nohynek, L., Virkajarvi, I. Matto, J. (2004) Stationary-phase acid and heat treatments for improvement of the viability of probiotic lactobacilli and bifidobacteria. J Appl Microbiol 96, 1205 1214. (Pubitemid 38703599)
40. Santivarangkna, C., Kulozik, U. Foerst, P. (2007) Alternative drying processes for the industrial preservation of lactic acid starter cultures. Biotechnol Prog 23, 302 315. (Pubitemid 46626302)
41. Saxelin, M., Grenov, B., Svensson, U., Fonden, R., Reniero, R. Mattila-Sandholm, T. (1999) The technology of probiotics. Trends Food Sci Technol 10, 387 392.
42. Schiraldi, C., Di Lernia, I. De Rosa, M. (2002) Trehalose production: exploiting novel approaches. Trends Biotechnol 20, 420 425. (Pubitemid 35239934)
43. Schoug, A., Olsson, J., Carlfors, J., Schnürer, J. Håkansson, S. (2006) Freeze-drying of Lactobacillus coryniformis Si3-effects of sucrose concentration, cell density, and freezing rate on cell survival and thermophysical properties. Cryobiology 53, 119 127. (Pubitemid 44037558)
44. Selmer-Olsen, E., Birkeland, S.E. Sørhaug, T. (1999a) Effect of protective solutes on leakage from and survival of immobilized Lactobacillus subjected to drying, storage and rehydration. J Appl Microbiol 87, 429 437. (Pubitemid 29479833)
45. Selmer-Olsen, E., Sørhaug, T., Birkeland, S.E. Pehrson, R. (1999b) Survival of Lactobacillus helveticus entrapped in Ca-alginate in relation to water content, storage and rehydration. J Ind Microbiol Biot 23, 79 85. (Pubitemid 29462143)
46. Smirnoff, N. Cumbes, Q.J. (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28, 1057 1060.
47. To, B.C.S. Etzel, M.R. (1997) Spray drying, freeze drying, or freezing of three different lactic acid bacteria species. J Food Sci 62, 576 578.
48. Wang, Y.C., Yu, R.C. Chou, C.C. (2004) Viability of lactic acid bacteria and bifidobacteria in fermented soymilk after drying, subsequent rehydration and storage. Int J Food Microbiol 93, 209 217.
49. Zamora, L.M., Carretero, C. Pares, D. (2006) Comparative survival rates of lactic acid bacteria isolated from blood, following spray-drying and freeze-drying. Food Sci Technol Int 12, 77 84.
50. Zarate, G. Nader-Macias, M.E. (2006) Viability and biological properties of probiotic vaginal Lactobacilli after lyophilization and refrigerated storage into gelatin capsules. Process Biochem 41, 1779 1785. (Pubitemid 43767161)
51. Zayed, G. Roos, Y.H. (2004) Influence of trehalose and moisture content on survival of Lactobacillus salivarius subjected to freeze-drying and storage. Process Biochem 39, 1081 1086. (Pubitemid 38527179)