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Note: indeed the resolution of our reconstructions is better than 5 nm (1 μm thick specimen) and 50 nm (4 μm thick specimen), respectively, and distances between gold beads smaller than their diameter can be identified, however it is difficult to prove the actual resolution in the thickness direction of a reconstruction. The bottom beads show less contrast compared with the top beads; however, sharpness and size still are similar.
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Note: indeed the volume resolution of our reconstructions is better than 5 nm (1 μm thick specimen) and 50 nm (4 μm thick specimen), respectively, and distances between gold beads smaller than their diameter can be identified, however it is difficult to prove the actual resolution in the thickness direction of a volume reconstruction. The bottom beads show less contrast compared with the top beads; however, sharpness and size still are similar.
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