Neutrase immobilization on alginate-glutaraldehyde beads by covalent attachment

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 1, 2009, Pages 109-115

  Ortega, Nattvidad ^a   Perez Mateos, Manuel ^a   Pilar, María C ^a   Busto, María D ^a  

^a UNIVERSITY OF BURGOS   (Spain)

Author keywords

Alginate glutaraldehyde beads; Covalent immobilization; Factorial design; Neutrase; Response surface methodology

Indexed keywords

BACILLUS SUBTILIS;

ALGINIC ACID; GLUCURONIC ACID; GLUTARALDEHYDE; HEXURONIC ACID; IMMOBILIZED ENZYME; METALLOPROTEINASE; MICROBIAL METALLOPROTEINASES; MICROSPHERE;

ARTICLE; ENZYME STABILITY; HEAT; KINETICS; METABOLISM; PH; TIME;

ALGINATES; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLUCURONIC ACID; GLUTARAL; HEXURONIC ACIDS; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; KINETICS; METALLOENDOPEPTIDASES; MICROSPHERES; TIME FACTORS;

EID: 59849124620     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf8015738     Document Type: Article

References (40)

1. Wiseman, A. Designer enzyme and cell applications in industry and environmental monitoring. J. Chem. Technol. Biotechnol. 1993, 56, 3-13.
2. Godfrey, T. Protein modification. In Industrial Enzymology; Godfrey, T., West, S., Eds.; MacMillan Press: London, U.K., 1996; pp 301-325.
3. Vioque, J.; Sanchez-Vioque, R.; Clemente, A.; Pedroche, J.; Millán, F. Partially hydrolyzed rapeseed protein isolates with improved functional properties. J. Am. Oil Chem. Soc. 2000, 77, 447-450.
4. Smyth, M.; FitzGerald, R. J. Relationship between some characteristics of WPC hydrolysates and the enzyme complement in commercially available proteinase preparations. Int. Dairy J. 1998, 8, 819-827.
5. Li, G. H.; Le, G. W.; Liu, H.; Shi, Y. H. Mung-bean protein hydrolysates obtained with alcalase exhibit angiotensin I-converting enzyme inhibitory activity. Food Sci. Technol. Int. 2005, 11, 281-287.
6. Apar, D. K.; Özbek, B. Hydrolysis and solubilisation of corn gluten by Neutrase. J. Chem. Technol. Biotechnol. 2007, 82, 1107-1114.
7. Shin, H. S.; Kim, S. B.; Kang, S. C.; Khan, M. A.; Kim, H. S.; Shin, H. J.; Chang, C. H. Production of low antigenic cheese whey protein hydrolysates using mixed proteolytic enzymes. J. Sci. Food Agric. 2007, 87, 2055-2060.
8. Raghavan, S.; Kristinsson, H. G. Antioxidative efficacy of alkali-treated tilapia protein hydrolysates: a comparative study of five enzymes. J. Agric. Food Chem. 2008, 56, 1434-1441.
9. Bao, X.-L.; Lv, Y.; Yang, B.-C.; Ren, G.-G.; Guo, S.-T. A study of the soluble complexed formed during calcium binding by soybean protein hydrolysates. J. Food Sci. 2008, 73, C117-C121.
10. Kumar, H. R. H.; Monteiro, P. V.; Bhat, G. S.; Rao, H. G. R. Effects of enzymatic modification of milk proteins on flavour and textural qualities of set yoghurt. J. Sci. Food Agric. 2000, 81, 42-45.
11. Clapés, P.; Pera, E.; Torres, J. L. Peptide bond formation by the industrial protease, Neutrase, in organic media. Biotechnol. Lett. 1997, 19, 1023-1026.
12. Le-Tien, C.; Millette, M.; Lacroix, M.; Mateescu, M.-A. Modified alginate matrices for the immobilization of bioactive agents. Biotechnol. Appl. Biochem. 2004, 39, 189-198.
13. Yeom, C. K.; Lee, K. H. Characterization of sodium alginate membrane crosslinked with glutaraldehyde in pervaporation separation. J. Appl. Polym. Sci. 1998, 67, 209-219.
14. Ates, S.; Mehmetoglu, U. A new method for immobilization of β-galactosidase and its utilization in a plug flow reactor. Process Biochem. 1997, 32, 433-436.
15. Naganagouda, K.; Mulimani, V. H. Gelatin blends with alginate: Gel fibers for α-galactosidase immobilization and its application in reduction of non-digestible oligosaccharides in soymilk. Process Biochem. 2006, 41, 1903-1907.
16. Nighojkar, S.; Phanse, Y.; Sinha, D.; Nighojkar, A.; Kumar, A. Production of polygalacturonase by immobilized cells of Aspergillus niger using orange peel. Process Biochem. 2006, 41, 1136-1140.
17. Lamas, E. M.; Barros, R. M.; Balcão, V. M.; Malcata, F. X. Hydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supports. Enzyme Microb. Technol. 2001, 28, 642-652.
18. Barros, R. M.; Extremina, C.; Conçalves, I. C.; Braga, B. O.; Balcão, V. M.; Malcata, F. X. Hydrolysis of α-lactoalbumin by cardosin A immobilize on highly activated supports. Enzyme Microb. Technol. 2003, 7, 908-916.
19. Rozie, H.; Somers, W.; Bonte, A.; Visser, J.; Van't Riet, K.; Rombouts, F. M. Adsorption characteristics of endo-polygalacturonase on alginate beads. Biotechnol. Appl. Biochem. 1988, 10, 346-358.
20. Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951, 193, 265-275.
21. Haaland, P. D. Experimental Design in Biotechnology; Dekker: New York, 1989.
22. Montgomery, D. C. Diseño de Análisis de Experimentos; Iberoamericana: Mexico City, Mexico, 1991.
23. Chandrika, G. P. Crosslinking of enzymes for improved stability and performance. Curr. Opin. Biotechnol. 1999, 10, 331-335.
24. Mateo, C.; Abian, O.; Bernedo, M.; Cuenca, E.; Fuentes, M.; Fernandez-Lorente, G.; Palomo, J. M.; Grazu, V.; Pessela, B. C. C.; Giacomini, C.; Irazoqui, G.; Villarino, A.; Ovsejevi, K.; Batista-Viera, F.; Fernandez-Lafuente, R.; Guisán, J. M. Some special features of glyoxyl supports to immobilize proteins. Enzyme Microb. Technol. 2005, 37, 456-462.
25. Betancor, L.; López-Gallego, F.; Hidalgo, A.; Alonso-Morales, N.; Dellamora-Ortiz, G.; Mateo, C.; Fernández-Lafuente, R.; Guisán, J. M. Different mechanisms of protein immobilization on glutaraldehyde activated supports: effect of support activation and immobilization conditions. Enzyme Microb. Technol. 2006, 39, 877-882.
26. Silva, C. J. S. M.; Zhang, Q.; Shen, J.; Cavaco-Paulo, A. Immobilization of proteases with a water soluble-insoluble reversible polymer for treatment of wool. Enzyme Microb. Technol. 2006, 39, 634-640.
27. Yust, M. M.; Pedroche, J.; Alaiz, M.; Girón-Calle, J.; Vioque, J.; Mateo, C.; Guisán, J. M.; Millán, F.; Fernández-Lafuente, R. Partial purification and immobilization/stabilization on highly activated glyoxyl-agarose supports of different proteases from flavourzyme. J. Agric. Food Chem. 2007, 55, 6503-6508.
28. Hayashi, T.; Hyon, S.-H.; Cha, W.-I.; Ikada, Y. Immobilization of thiol proteases onto porous poly(vinyl alchohol) beads. Polym. J. 1993, 25, 489-497.
29. Jeohn, T.-H.; Matsuzaki, H.; Takahashi, K. Purification and characterization of a detergent-requering membrane-bound metalloendopeptidase from porcine brain. Eur. J. Biochem. 1999, 260, 318-324.
30. Perrin, D. D.; Dempsey, B. Buffers for pH and Metal Ion Control; Chapman and Hall: London, U.K., 1974.
31. Mateo, C.; Palomo, J. M.; Fernandez-Lorente, G.; Guisan, J. M.; Fernandez-Lafuente, R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb. Technol. 2007, 40, 1451-1463.
32. Tanksale, A.; Chandra, P. M.; Rao, M.; Deshpande, V. Immobilization of alkaline protease from Conidiobolus macrosporus for reuse and improved thermal stability. Biotechnol. Lett. 2001, 23, 51-54.
33. Rao, R. S.; Borkar, P. S.; Khobragade, C. N.; Sagar, A. D. Enzymatic activities of proteases immobilized on tri(4-formyl phenoxy) cyanurate. Enzyme Microb. Technol. 2006, 39, 958-962.
34. Alton, G. D.; Cetinus, S. A. Immobilization of pepsin on chitosan beads. Food Chem. 2007, 100, 964-971.
35. Li, J.; Du, Y.; Sun, L.; Liang, H.; Feng, T.; Wein, Y.; Yao, P. Chitosaneous hydrogel beads for immobilizing neutral protease for application in the preparation of low molecular weight chitosan and chito-oligomers. J. Appl. Polym. Sci. 2006, 101, 3743-3750.
36. Ping, W.; Sheng, D.; Waezsada, S. D.; Tsao, A. Y.; Davinson, B. H. Enzyme stabilization by covalent binding in nanoporous sol-gel glass for nonaqueous biocatalysis. Biotechnol. Bioeng. 2001, 74, 249-255.
37. Mozhaev, V. V.; Martinek, K. Structure stability relationship in protein: a guide to approaches to stabilizing enzymes. Adv. Drug Delivery Rev. 1990, 4, 387-419.
38. Mozhaev, V. V. Mechanism based strategies for protein thermostabilization. Trends Biotechnol. 1993, 11, 88-95.
39. Iyer, P. V.; Ananthanarayan, L. Enzyme stability and stabilization - aqueous and non aqueous enrionment. Process Biochem. 2008, 43, 1019-1032.
40. Yong, Y.; Bai, Y.-X.; Li, Y.-F.; Lin, L.; Cui, Y.-J.; Xia, C.-G. Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity. Process Biochem. 2008, 43, 1179-1185.