Purification and characterization of a detergent-requiring membrane- bound metalloendopeptidase from porcine brain

European Journal of Biochemistry

Volumn 260, Issue 2, 1999, Pages 318-324

  Jeohn, Gwang Ho ^b   Matsuzaki, Hidemi ^b   Takahashi, Kenji ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b NONE

Author keywords

Detergent requiring protease; Membrane bound protease; Metalloendopeptidase; Porcine brain; Progastrin peptide

Indexed keywords

1,10 PHENANTHROLINE; BAM 12 P; BRADYKININ; DETERGENT; DYNORPHIN A; EDETIC ACID; EGTAZIC ACID; LUBROL; MEMBRANE ENZYME; METALLOPROTEINASE; NEUROLYSIN; NEUROPEPTIDE; OLIGOPEPTIDE; PROGASTRIN; TRITON X 100;

ANIMAL TISSUE; ARTICLE; BRAIN; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; HYDROLYSIS; MEMBRANE VESICLE; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; SWINE;

AMINO ACID SEQUENCE; ANIMALS; BRAIN; CENTRIFUGATION, DENSITY GRADIENT; COBALT; COUMARINS; DETERGENTS; DYNORPHINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; INTRACELLULAR MEMBRANES; MANGANESE; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OLIGOPEPTIDES; PROTEASE INHIBITORS; SOLUBILITY; SUBSTRATE SPECIFICITY; SWINE; ZINC;

ANIMALIA; SUIDAE; SUS SCROFA;

EID: 0033104962     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00151.x     Document Type: Article

References (18)

1. Steiner, D.F., Smeekens, S.P., Ohagi, S. & Chan, S.J. (1992) The new enzymology of precursor processing endopeptidases. J. Biol. Chem. 267, 23435-23438.
2. Barr, P.J. (1991) Mammalian subtilisins: The long-sought dibasic processing endoproteases. Cell 66, 1-3.
3. Dickinson, C.J., Daugherty, D., Guo, Y.-J., Hughes, P. & Yamada, T. (1992) Molecular analysis of dibasic endoproteolytic cleavage signals. J. Biol. Chem. 267, 21795-21801.
4. Mizuno, K., Kojima, M. & Matsuo, H. (1985) A putative prohormone-processing protease in bovine adrenal medulla specifically cleaving in between Lys-Arg sequences. Biochem. Biophys. Res. Commun. 128, 884-891.
5. Parish, D.C., Tuteja, R., Altstein, M., Gainer, H. & Loh, Y.P. (1986) Purification and characterization of a paired basic residue-specific prohormone-converting enzyme from bovine pituitary neural lobe secretory vesicles. J. Biol. Chem. 261, 14392-14397.
6. Estivariz, F.E., Friedman, T.C., Chikuma, T. & Loh, Y.P. (1992) Processing of adrenocorticotropin by two proteases in bovine intermediate lobe secretory vesicle membranes. A distinct acidic, tetrabasic residue-specific calcium-activated serine protease and a PC2-like enzyme. J. Biol. Chem. 267, 7456-7463.
7. Yoo, O.J., Powell, C.T. & Agarwal, K.L. (1982) Molecular cloning and nucleotide sequence of full-length of cDNA coding for porcine gastrin. Proc. Natl. Acad. Sci. USA 79, 1049-1053.
8. Boel, E., Vuust, J., Norris, F., Norris, K., Wind, A., Rehfeld, J.F. & Marker, K.A. (1983) Molecular cloning of human gastrin cDNA: evidence for evolution of gastrin by gene duplication. Proc. Natl Acad. Sci. USA 80, 2866-2869.
9. Jensen, S., Borch, K., Hilsted, L. & Rehfeld, J.F. (1989) Progastrin processing during antral G-cell hypersecretion in humans. Gastroenterology 96, 1063-1070.
10. Perrin, D.D. & Dempsey, B. (1974) Buffers for pH and Metal Ion Control, p. 48. Chapman & Hall Ltd., London.
11. Smith, P.K., Krohn, R.I., Hermanson, G.T. Mallia, A.K., Gartner, H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olsen, B.J. & Klenk, D.C. (1985) Measurement of protein using biscinchoninic acid. Anal. Biochem. 150, 76-85.
12. Jeohn, G.-H., Serizawa, S., Iwamatsu, A. & Takahashi, K. (1995) Isolation and characterization of gastric trypsin from the microsomal fraction of porcine gastric antral mucosa. J. Biol. Chem. 270, 14748-14755.
13. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
14. Barelli, H., Vincent, J.-P. & Checler, F. (1988) Peripheral inactivation of neurotensin. Isolation and characterization of a metallopeptidase from rat ileum. Eur. J. Biochem. 175, 481-489.
15. Rodd, D. & Hersh, L.B. (1995) Endopeptidase 24.16B. A new variant of endopeptidase 24.16. J. Biol. Chem. 270, 10056-10061.
16. Barrett, A.J. (1995) Proteolytic enzymes: aspartic and metallo peptidases. Methods Enzymol. 248, Academic Press, San Diego, USA.
17. Jeohn, G.-H. & Takahashi, K. (1995) Purification and characterization of a vasoactive intestinal polypeptide-degrading endoprotease from porcine antral mucosal membranes. J. Biol. Chem. 270, 7809-7815.
18. 16 bond in β-amyloid peptides by porcine vasoactive intestinal polypeptide-degrading endopeptidase. Prot. Peptides Lett. 3, 101-106.