메뉴 건너뛰기




Volumn 15, Issue 2, 2009, Pages 449-456

Nano-conjugate fluorescence probe for the discrimination of phosphate and pyrophosphate

Author keywords

Cobalt; Fluorescence; Iron; Nanostructures; Phosphates; Pyrophosphate

Indexed keywords

BINDING ENERGY; CARBOXYLATION; COBALT; NANOSTRUCTURES; PHOSPHATES; PROBES; TRANSITION METALS;

EID: 58449117955     PISSN: 09476539     EISSN: 15213765     Source Type: Journal    
DOI: 10.1002/chem.200801403     Document Type: Article
Times cited : (48)

References (77)
  • 6
    • 0035185097 scopus 로고    scopus 로고
    • E. Chantelau, VASA 2001, 30(S58), 15.
    • (2001) VASA , vol.30 , Issue.S58 , pp. 15
    • Chantelau, E.1
  • 8
    • 0004293337 scopus 로고    scopus 로고
    • Eds, B. Ratner, A. S. Hoffman, F.J. Schoen, J. E. Lemons, Academic Press, London
    • Biomaterials Science (Eds.: B. Ratner, A. S. Hoffman, F.J. Schoen, J. E. Lemons), Academic Press, London, 1996.
    • (1996) Biomaterials Science
  • 10
    • 58449088257 scopus 로고    scopus 로고
    • S. L. Leiboff, J. Biol. Chem. 1928, 79, 611.
    • S. L. Leiboff, J. Biol. Chem. 1928, 79, 611.
  • 22
    • 0037427327 scopus 로고    scopus 로고
    • S. L. Tobey, B. D. Jones, E. V. Anslyn, J. Am. Chem. Soc. 2003, 125, 4026;
    • b) S. L. Tobey, B. D. Jones, E. V. Anslyn, J. Am. Chem. Soc. 2003, 125, 4026;
  • 24
    • 34848817363 scopus 로고    scopus 로고
    • E. Quinlan, S. E. Matthews, T. Gunnlaugsson, J. Org. Chem. 2007, 72, 7497.
    • d) E. Quinlan, S. E. Matthews, T. Gunnlaugsson, J. Org. Chem. 2007, 72, 7497.
  • 46
    • 33644946681 scopus 로고    scopus 로고
    • I.-.B. Kim, U. H. F. Bunz, J. Am. Chem. Soc. 2006, 128, 2818;
    • a) I.-.B. Kim, U. H. F. Bunz, J. Am. Chem. Soc. 2006, 128, 2818;
  • 57
    • 58449105199 scopus 로고    scopus 로고
    • Ph.D. Thesis, Georgia Institute of Technology, Atlanta
    • b) E. L.-H. Heintz, Ph.D. Thesis, Georgia Institute of Technology, Atlanta, 2004.
    • (2004)
    • Heintz, E.L.-H.1
  • 64
    • 58449109832 scopus 로고    scopus 로고
    • Since the quenching of the fluorescence of 1. by the NP could be due to internal filter effects as a consequence of the absorption of the NP, we measured the emission spectra of the solutions of the PPE-NP assemblies after precipitation using a magnet. Under a magnetic field, free NPs and PPE 1 bound NPs were removed. The supernatant was effectively nonfluorescent after addition of NPs [40 pM], indicating the formation of the PPE-NP assemblies. The PPE-NP assemblies are easily re-dispersed into the solution by shaking in the absence of a magnetic field. The model compound 2 was not quenched by the NPs. The electrostatic interaction between 2 and NPs is insufficient to form a stable complex in the presence of buffer.
    • Since the quenching of the fluorescence of 1. by the NP could be due to internal filter effects as a consequence of the absorption of the NP, we measured the emission spectra of the solutions of the PPE-NP assemblies after precipitation using a magnet. Under a magnetic field, free NPs and PPE 1 bound NPs were removed. The supernatant was effectively nonfluorescent after addition of NPs [40 pM], indicating the formation of the PPE-NP assemblies. The PPE-NP assemblies are easily re-dispersed into the solution by shaking in the absence of a magnetic field. The model compound 2 was not quenched by the NPs. The electrostatic interaction between 2 and NPs is insufficient to form a stable complex in the presence of buffer.
  • 68
    • 58449113969 scopus 로고    scopus 로고
    • the Sigma quality control test procedure for the enzymatic assay of inorganic pyrophosphatase was employed
    • b) the Sigma quality control test procedure for the enzymatic assay of inorganic pyrophosphatase was employed.
  • 69
    • 58449087698 scopus 로고    scopus 로고
    • A small aliquot of the assay was transferred to the solution of the assemblies at set time intervals. The fluorescence of the solution is measured after 20 min of incubation time. The initial concentration of sodium PPi in the assay was 1.6 mM [1.0 mM, One unit of pyrophosphatase will liberate 1.0 μmol of inorganic Pi per minute at pH 9.0 at 50°C The fluorescence recovery from the solution gradually decreased with increasing incubation time. Beyond a specific incubation time of the assay 3 min for EA200; 5 min for EA100; 1.8 min for EA70, 30 min EA 40, the fluorescence intensity of the solution remained constant, indicating that all of the PPi had been hydrolyzed by the enzyme. No further fluorescence recovery was observed
    • A small aliquot of the assay was transferred to the solution of the assemblies at set time intervals. The fluorescence of the solution is measured after 20 min of incubation time. The initial concentration of sodium PPi in the assay was 1.6 mM [1.0 mM]. One "unit" of pyrophosphatase will liberate 1.0 μmol of inorganic Pi per minute at pH 9.0 at 50°C The fluorescence recovery from the solution gradually decreased with increasing incubation time. Beyond a specific incubation time of the assay (3 min for EA200; 5 min for EA100; 1.8 min for EA70, 30 min EA 40), the fluorescence intensity of the solution remained constant, indicating that all of the PPi had been hydrolyzed by the enzyme. No further fluorescence recovery was observed.
  • 70
    • 58449117234 scopus 로고    scopus 로고
    • o/F was plotted as a function of incubation time in each experiment.
    • o/F was plotted as a function of incubation time in each experiment.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.