Fluorescence sensing of a ribonucleoside 5'-triphosphate

Tetrahedron Letters

Volumn 38, Issue 36, 1997, Pages 6323-6326

  Patterson, Steven ^a   Smith, Bradley D ^a   Taylor, Richard E ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEOSIDE TRIPHOSPHATE;

ARTICLE; DRUG IDENTIFICATION; FLUORESCENCE; TECHNIQUE;

EID: 0030765944     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(97)01454-8     Document Type: Article

References (14)

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5. 2 and a photochromic diarylethene; Takeshita, M.; Uchida, K.; Irie, M. J. Chem. Soc., Chem. Commun. 1996, 1807-1808.
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10. +, 12), 366 (13), 191 (100); HRMS 557.2788 (calcd for C36H36BNO4: 557.2744).
11. Association constants were determined by the Benesi-Hildebrand method or nonlinear curve fitting of the equation for 1:1 binding. Tsukube, H.; Furata, H.; Odani, A.; Takeda, Y.; Kudo, Y.; Inoue, Y.; Liu, Y.; Sakamoto, H.; Kimura, K. in Comprehensive Supramolecular Chemistry, Atwood, J. L., Davies, J. E. D., MacNicol, D. D., Vögtle, F., Eds.; Pergamon, New York, 1996, vol. 8, Ch. 10.
12. -1) was redissolved in methanol/water to a make stock solution that was 20 μM in sensor unit. A 200 μL solution of this stock solution was diluted to 3.0 mL with phosphate buffer (15 mM, pH 7.3) to give a final sensor unit concentration of 1.3 μM.
13. Metzger, A.; Lynch, V. M.; Anslyn, E. V. Angew. Chem. Int. Ed. Engl. 1997, 36, 862-865.
14. Lewis, F. D., Zhang, Y.; Letsinger, R. L. J. Am. Chem. Soc., 1997, 119, 5451-5452.