Crystal structures of human Δ4-3-ketosteroid 5β-reductase (AKR1D1) reveal the presence of an alternative binding site responsible for substrate inhibition

Biochemistry

Volumn 47, Issue 51, 2008, Pages 13537-13546

  Faucher, Frédérick ^a,b   Cantin, Line ^a   Luu The, Van ^a   Labrie, Fernand ^a   Breton, Rock ^a  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BILE ACIDS; BINARY COMPLEXES; BINDING CAVITIES; BINDING MODES; BIO-CHEMICALS; CATALYTIC SITES; COFACTOR; DOUBLE BONDS; ENZYME INTERACTIONS; FLEXIBLE LOOPS; HYDROXYSTEROID DEHYDROGENASES; KINETIC CHARACTERIZATIONS; SAFETY BELTS; STEREOSPECIFIC MANNERS; STEROID HORMONES; STRUCTURAL COMPARISONS; STRUCTURAL INSIGHTS; STRUCTURAL STUDIES; SUBSTRATE INHIBITIONS; SUBSTRATE SPECIFICITIES; TERNARY COMPLEXES;

AMINO ACIDS; BINDING ENERGY; CATALYSTS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYMES; HORMONES; PROTEINS; SUBSTRATES;

BINDING SITES;

17 ALPHA HYDROXYSTEROID DEHYDROGENASE; 3ALPHA HYDROXYSTEROID DEHYDROGENASE; ANDROSTENEDIONE; OXIDOREDUCTASE; PHENYLALANINE; PROGESTERONE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; STEROID 5BETA REDUCTASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; HUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; HUMANS; KINETICS; MOLECULAR CONFORMATION; MUTAGENESIS, SITE-DIRECTED; NADP; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TESTOSTERONE 5-ALPHA-REDUCTASE;

EID: 58149171909     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801276h     Document Type: Article

References (45)

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