A coupled assay measuring Mycobacterium tuberculosis antigen 85C enzymatic activity

Analytical Biochemistry

Volumn 385, Issue 1, 2009, Pages 120-127

  Boucau, Julie ^a   Sanki, Aditya K ^a   Voss, Bradley J ^b   Sucheck, Steven J ^a   Ronning, Donald R ^a  

^a UNIVERSITY OF TOLEDO   (United States)

^b 320 South Main St Olivet College   (United States)

Author keywords

Acyltransfer; Antigen 85C; Drug screening; Enzyme coupled assay; Mycolyltransferase; Glucosidase

Indexed keywords

ANTIGENS; CARBOHYDRATES; CHROMOPHORES; DRUG PRODUCTS; ENZYMES; FATTY ACIDS; SILVER COMPOUNDS; TARGETED DRUG DELIVERY;

ACYLTRANSFER; DRUG DISCOVERY PROCESS; DRUG SCREENING; DRUG-RESISTANT STRAINS; GLUCOSIDASE; HIGH THROUGHPUT SCREENING; MYCOBACTERIUM TUBERCULOSIS; MYCOLYLTRANSFERASE;

CONTROLLED DRUG DELIVERY;

ANTIGEN 85 ENZYME; BACTERIAL ENZYME; BETA GLUCOSIDASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM TUBERCULOSIS;

EID: 57749190807     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.10.018     Document Type: Article

References (27)

1. Gandhi N.R., Moll A., Sturm A.W., Pawinski R., Govender T., Lalloo U., Zeller K., Andrews J., and Friedland G. Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa. Lancet 368 (2006) 1575-1580
2. Draper P., and Daffe M. The cell envelope of Mycobacterium tuberculosis with special reference to the capsule and outer membrane permeability barrier. In: Cole S.T., Eisenach K.D., and McMurray D.N. (Eds). Tuberculosis and the Tubercle Bacillus (2005), ASM Press, Washington, DC 261-273
3. Zhang Y., Vilcheze C., and Jacobs W.R. Mechanisms of drug resistance in Mycobacterium tuberculosis. In: Cole S.T., Eisenach K.D., and McMurray D.N. (Eds). Tuberculosis and the Tubercle Bacillus (2005), ASM Press, Washington, DC 115-140
4. Yoneda M., and Fukui Y. Isolation, purification, and characterization of extracellular antigens of Mycobacterium tuberculosis. Am. Rev. Respir. Med. 92 (1965) 9-18
5. Harth G., Lee B.Y., Wang J., Clemens D.L., and Horwitz M.A. Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis,. Infect. Immun. 64 (1996) 3038-3047 (Erratum appears in Infect. Immun. (1997) 852)
6. Belisle J.T., Vissa V.D., Sievert T., Takayama K., Brennan P.J., and Besra G.S. Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis. Science 276 (1997) 1420-1422
7. Jackson M., Raynaud C., Laneelle M.A., Guilhot C., Laurent-Winter C., Ensergueix D., Gicquel B., and Daffe M. Inactivation of the antigen 85C gene profoundly affects the mycolate content and alters the permeability of the Mycobacterium tuberculosis cell envelope. Mol. Microbiol. 31 (1999) 1573-1587
8. Puech V., Guilhot C., Perez E., Tropis M., Armitige L.Y., Gicquel B., and Daffe M. Evidence for a partial redundancy of the fibronectin-binding proteins for the transfer of mycolyl residues onto the cell wall arabinogalactan termini of Mycobacterium tuberculosis. Mol. Microbiol. 44 (2002) 1109-1122
9. Harth G., Horwitz M.A., Tabatadze D., and Zamecnik P.C. Targeting the Mycobacterium tuberculosis 30/32-kDa mycolyl transferase complex as a therapeutic strategy against tuberculosis: Proof of principle by using antisense technology. Proc. Natl. Acad. Sci. USA 99 (2002) 15614-15619
10. Harth G., Zamecnik P.C., Tabatadze D., Pierson K., and Horwitz M.A. Hairpin extensions enhance the efficacy of mycolyl transferase-specific antisense oligonucleotides targeting Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 104 (2007) 7199-7204
11. Hanoulle X., Wieruszeski J.M., Rousselot-Pailley P., Landrieu I., Locht C., Lippens G., and Baulard A.R. Selective intracellular accumulation of the major metabolite issued from the activation of the prodrug ethionamide in mycobacteria. J. Antimicrob. Chemother. 58 (2006) 768-772
12. Alahari A., Trivelli X., Guerardel Y., Dover L.G., Besra G.S., Sacchettini J.C., Reynolds R.C., Coxon G.D., and Kremer L. Thiacetazone, An antitubercular drug that inhibits cyclopropanation of cell wall mycolic acids in mycobacteria. PLoS ONE 2 (2007) e1343
13. Goddard J.P., and Reymond J.L. Recent advances in enzyme assays. Trends Biotechnol. 22 (2004) 363-370
14. Ronning D.R., Vissa V., Besra G.S., Belisle J.T., and Sacchettini J.C. Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity. J. Biol. Chem. 279 (2004) 36771-36777
15. Ronning D.R., Klabunde T., Besra G.S., Vissa V.D., Belisle J.T., and Sacchettini J.C. Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines. Nat. Struct. Biol. 7 (2000) 141-146
16. Anderson D.H., Harth G., Horwitz M., and Eisenberg D. An interfacial mechanism and a class of inhibitors inferred from two crystal structures of the Mycobacterium tuberculosis 30 kDa major secretory protein (antigen 85B), a mycolyl transferase. J. Mol. Biol. 307 (2001) 671-681
17. Wu C., Chang C., Chen J., Wong C., and Lin C. Rapid diversity-oriented synthesis in microtiter plates for in situ screening: Discovery of potent and selective α-fucosidase inhibitors. Angew. Chem. Intl. Ed. 42 (2003) 4661-4664
18. Weadge J.T., and Clarke A.J. Neisseria gonorrheae O-acetylpeptidoglycan esterase. A serine esterase with a Ser-His-Asp catalytic triad, Biochemistry 46 (2007) 4932-4941
19. Armarego W.L.F., and Chai C.L.L. Purification of Organic Chemicals. Purification of Laboratory Chemicals (2003), Butterworth-Heinemann, New York pp. 80-388
20. Plusquellec D., and Baczko K. Sugar chemistry without protecting groups: a novel regioselective synthesis of 6-O-acyl-D-glucopyranoses and methyl-6-O-acyl-α-D-glucopyranosides. Tetrahedron Lett. 28 (1987) 3809-3812
21. Plusquellec D., Roulleau F., Bertho F., Lefeuvre M., and Brown E. Chemistry of sugars without protective groups: I. Regioselective esterification of the anomeric hydroxyl of lactose, maltose, and glucose. Tetrahedron 42 (1986) 2457-2467
22. Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., and Bairoch A. Protein identification and analysis tools on the ExPASy server. In: Walker J.M. (Ed). The Proteomics Protocols Handbook (2005), Humana Press, Totowa, NJ 571-607
23. Zhang J.-H., Chung T.D.Y., and Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4 (1999) 67-73
24. Rosenberry T.L., and Bernhard S.A. Catalysis by acetylcholinesterase: I. Fluorescent titration with a carbamoylating agent. Biochemistry 10 (1971) 4114-4120
25. Trauger J.W., Kohli R.M., Mootz H.D., Marahiel M.A., and Walsh C.T. Peptide cyclization catalysed by the thioesterase domain of tyrocidine synthetase. Nature 407 (2000) 215-218
26. Park Y.J., Choi S.Y., and Lee H.B. A carboxylesterase from the thermoacidophilic archaeon Sulfolobus solfataricus P1: Purification, characterization, and expression. Biochim. Biophys. Acta 1760 (2006) 820-828
27. A.K. Sanki, J. Boucau, D.R. Ronning, S.J. Sucheck, Antigen 85C-mediated acyl-transfer between synthetic acyl donors and fragments of the arabinan, Glycoconj. J. (in press), doi:10.1007/s10719-008-9211-z.