Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis

Infection and Immunity

Volumn 64, Issue 8, 1996, Pages 3038-3047

  Harth, Günter ^a   Lee, Bai Yu ^a   Wang, Jun ^a   Clemens, Daniel L ^a   Horwitz, Marcus A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BCG VACCINE; SECRETORY PROTEIN; SIGNAL PEPTIDE;

ARTICLE; BACTERIAL CELL WALL; BACTERIAL GENE; CONTROLLED STUDY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; MYCOBACTERIUM BOVIS; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; PROTEIN INDUCTION; PROTEIN LOCALIZATION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION; TUBERCULOSIS;

EID: 0030056239     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.8.3038-3047.1996     Document Type: Article

References (29)

1. Allison, A. C., and N. E. Byars. 1986. An adjuvant formulation that selectively elicits the formation of antibodies of protective isotypes and of cell-mediated immunity. J. Immunol. Methods 95:157-168.
2. Belisle, J. T., T. Sievert, K. Takayama, and G. S. Besra. 1995. Identification of a mycolyltransferase from Mycobacterium tuberculosis and the coincident definition of the physiological function of antigen 85B, p. 212-216. In Program of the 30th joint Conference on Tuberculosis and Leprosy. U.S.-Japan Cooperative Medical Science Program, NIAID, NIH, Ft. Collins, Colo., 19-21 July 1995. Department of Health and Human Services, Washington, D.C.
3. Borremans, M., L. De Wit, G. Volckaert, J. Ooms, J. De Bryun, K. Huygen, J.-P. Van Vooren, M. Stelandre, R. Verhofstadt, and J. Content. 1989. Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis. Infect. Immun. 57:3123-3130.
4. Chen, E. Y., and P. H. Seeburg. 1985. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165-170.
5. Clemens, D. L., and M. A. Horwitz. 1995. Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited. J. Exp. Med. 181:257-270.
6. Content, J., A. de la Cuvellerie, L. De Wit, V. Vincent-Levy-Frébault, J. Ooms, and J. De Bruyn. 1991. The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG arc members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of Mycobacterium tuberculosis. Infect. Immun. 59:3205-3212.
7. De Mendonça-Lima, L., J. Content, H. van Heuverswyn, and W. Degrave. 1991. Nucleotide sequence of the gene coding for the 85-B antigen of Mycobacterium leprae. Nucleic Acids Res. 19:5789.
8. De Wit, L., M. Palou, and J. Content. 1994. Nucleotide sequence of the 85B-protein gene of Mycobacterium bovis BCG and Mycobacterium tuberculosis. DNA Sequence 4:267-270.
9. Grunstein, M., and D. S. Hogness. 1975. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA 72:3961-3966.
10. Harth, G., D. L. Clemens, and M. A. Horwitz. 1994. Glutamine synthetase of Mycobacterium tuberculosis: extracellular release and characterization of its enzymatic activity. Proc. Natl. Acad. Sci. USA 91:9342-9346.
11. Hatfull, G. F., and G. J. Sarkis. 1993. DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics. Mol. Microbiol. 7:395-405.
12. Horwitz, M. A., B.-W. E. Lee, B. J. Dillon, and G. Harth. 1995. Protective immunity against tuberculosis induced by vaccination with major extracellular proteins of Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 92:1530-1534.
13. Kitaura, H., N. Ohara, T. Matsuo, H. Tasaka, K. Kobayashi, and T. Yamada. 1993. Cloning, sequencing and expression of the gene for alpha antigen from Mycobacterium intracellulare and use of PCR for the rapid identification of Mycobacterium intracellulare. Biochem. Biophys. Res. Commun. 196:1466-1473.
14. Kremer, L., A. Baulard, J. Estaquier, J. Content, A. Capron, and C. Locht. 1995. Analysis of the Mycobacterium tuberculosis 85A antigen promoter region. J. Bacteriol. 177:642-653.
15. Kyte, J., and R. F. Doolittle. 1982. Simple method for displaying the hydropathy character of a protein. J. Mol. Biol. 157:105-132.
16. Launois, P., R. DeLeys, M'. N'Diaye Niang, A. Drowart, M. Andrien, P. Dierckx, J.-L. Cartel, J.-L. Sarthou, J.-P. Van Vooren, and K. Huygen. 1994. T-cell-epitope mapping of the major secreted mycobacterial antigen Ag85A in tuberculosis and leprosy. Infect. Immun. 62:3679-3687.
17. Lee, B.-Y., and M. A. Horwitz. 1995. Identification of macrophage and stress-induced proteins of Mycobacterium tuberculosis. J. Clin. Invest. 96:245-249.
18. Lee, T. D., and S. Vemuri. 1990. MacProMass: a computer program to correlate mass spectral data to peptide and protein structures. Biomed. Environ. Mass. Spectrom. 19:639-645.
19. Matsuo, K., R. Yamaguchi, A. Yamazaki, H. Tasaka, K. Terasaka, and T. Yamada. 1990. Cloning and expression of the gene for the cross-reactive alpha antigen of Mycobacterium kansasii. Infect. Immun. 58:550-556.
20. Matsuo, K., R. Yamaguchi, A. Yamazaki, H. Tasaka, and T. Yamada. 1988. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular α-antigen. J. Bacteriol. 170:3847-3854.
21. Ohara, N., K. Matsuo, R. Yamaguchi, A. Yamazaki, H. Tasaka, and T. Yamada. 1993. Cloning and sequencing of the gene for alpha antigen from Mycobacterium avium and mapping of B-cell epitopes. Infect. Immun. 61:1173-1179.
22. Pal, P. G., and M. A. Horwitz. 1992. Immunization with extracellular protein of Mycobacterium tuberculosis induces cell-mediated immune response and substantial protective immunity in a guinea pig model of pulmonary tuberculosis. Infect. Immun. 60:4781-4792.
23. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
24. Shine, J., and L. Dalgarno. 1974. The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. Natl. Acad. Sci. USA 71:1342-1346.
25. Von Heijne, G. 1986. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 4:4683-4690.
26. Weimbs, T., and W. Stoffel. 1992. Proteolipid protein (PLP) of CNS myelin: positions of free, disulflde-bonded, and fatty acid thioester-linked cysteine residues and implications for the membrane topology of PLP. Biochemistry 31:12289-12296.
27. Wiker, H. G., and M. Harboe. 1992. The antigen 85 complex: a major secretion product of Mycobacterium tuberculosis. Microbiol. Rev. 56:648-661.
28. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
29. Young, D. B., S. H. E. Kaufmann, P. W. M. Hermans, and J. E. R. Thole. 1992. Mycobacterial protein antigens: a compilation. Mol. Microbiol. 6:133-145.