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Volumn 276, Issue 5317, 1997, Pages 1420-1422

Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; AMINO ACID; ANTIGEN; CARBOXYLESTERASE; FIBRONECTIN; GENE PRODUCT; MONOCLONAL ANTIBODY; MYCOLIC ACID; PROTEIN; PYRANOSIDE; SILVER NITRATE; TRANSFERASE; TREHALOSE; TRIACYLGLYCEROL LIPASE;

EID: 0031007903     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5317.1420     Document Type: Article
Times cited : (639)

References (37)
  • 4
    • 0028064838 scopus 로고
    • B. A. Wolucka, M. R. McNeil, E. de Hoffmann, T. Chojnacki, P. J. Brennan, J. Biol. Chem. 269, 23328 (1994); K. Mikusova, M. Mikus, G. S. Besra, I. Hancock, P. J. Brennan, ibid. 271, 7820 (1996); R. E. Lee, K. Mikušová, P. J. Brennan, G. S. Besra, J. Am. Chem. Soc. 117, 11829 (1995).
    • (1994) J. Biol. Chem. , vol.269 , pp. 23328
    • Wolucka, B.A.1    McNeil, M.R.2    De Hoffmann, E.3    Chojnacki, T.4    Brennan, P.J.5
  • 5
    • 0029869839 scopus 로고    scopus 로고
    • B. A. Wolucka, M. R. McNeil, E. de Hoffmann, T. Chojnacki, P. J. Brennan, J. Biol. Chem. 269, 23328 (1994); K. Mikusova, M. Mikus, G. S. Besra, I. Hancock, P. J. Brennan, ibid. 271, 7820 (1996); R. E. Lee, K. Mikušová, P. J. Brennan, G. S. Besra, J. Am. Chem. Soc. 117, 11829 (1995).
    • (1996) J. Biol. Chem. , vol.271 , pp. 7820
    • Mikusova, K.1    Mikus, M.2    Besra, G.S.3    Hancock, I.4    Brennan, P.J.5
  • 6
    • 0029589845 scopus 로고
    • B. A. Wolucka, M. R. McNeil, E. de Hoffmann, T. Chojnacki, P. J. Brennan, J. Biol. Chem. 269, 23328 (1994); K. Mikusova, M. Mikus, G. S. Besra, I. Hancock, P. J. Brennan, ibid. 271, 7820 (1996); R. E. Lee, K. Mikušová, P. J. Brennan, G. S. Besra, J. Am. Chem. Soc. 117, 11829 (1995).
    • (1995) J. Am. Chem. Soc. , vol.117 , pp. 11829
    • Lee, R.E.1    Mikušová, K.2    Brennan, P.J.3    Besra, G.S.4
  • 8
    • 0029000868 scopus 로고
    • A. Dessen, A. Quemard, J. S. Blanchard, W. R. Jacobs Jr., J. C. Sacchettini, Science 267, 1638 (1995); A. Quemard et al., Biochemistry 34, 8235 (1995).
    • (1995) Biochemistry , vol.34 , pp. 8235
    • Quemard, A.1
  • 12
    • 1842316057 scopus 로고    scopus 로고
    • note
    • 3OH (2:1), and 50 μl was added to 5 ml of scintillation fluid and counted.
  • 14
    • 0011822726 scopus 로고    scopus 로고
    • Academic Press, San Diego, CA
    • N. C. Price, Ed., Protein Labfax (Academic Press, San Diego, CA, 1996), p. 270.
    • (1996) Protein Labfax , pp. 270
    • Price, N.C.1
  • 17
    • 1842308482 scopus 로고
    • H. G. Wiker and M. Harboe, Microbiol. Rev. 56, 66 (1992); M. A. Horwitz, B. W. Lee, B. J. Dillon, G. Harth, Proc. Natl. Acad. Sci. U.S.A. 92, 1530 (1995).
    • (1992) Microbiol. Rev. , vol.56 , pp. 66
    • Wiker, H.G.1    Harboe, M.2
  • 23
    • 1842386514 scopus 로고    scopus 로고
    • note
    • 4 (pH 7.5), 1 mM EDTA, 1 mM DTT], and 38 mg was applied to a column (1 cm by 10 cm) of Phenyl Sepharose (Pharmacia Biotech, Uppsala, Sweden). The column was washed with three volumes of storage buffer at a flow rate of 1 ml/min, which eluted most of the proteins while leaving the Ag85 complex bound to the Phenyl Sepharose matrix. The individual proteins of the Ag85 complex were eluted with 30 ml of buffer A [10 mM tris-HCl (pH 8.6), 1 mM DTT, 1 mM EDTA] followed by a linear gradient composed of 100% buffer A to 100% buffer B [10 mM tris-HCl (pH 8.6), 1 mM DTT, 1 mM EDTA, 50% ethylene glycol] over a 40-ml volume followed by 10 ml of 100% buffer B.
  • 28
    • 1842275849 scopus 로고    scopus 로고
    • note
    • 4 primers. This product was ligated into pET23b, as described for the nonmutated fbpC fragment, to yield the recombinant plasmid pCSB9sa. Fidelity of the pCSB9 and pCSB9sa constructs was determined by sequence analysis.
  • 32
    • 1842401448 scopus 로고    scopus 로고
    • note
    • 14C-labeled, delipidated cells, rich in the bound mycolate esters of arabinogalactan, were subjected to alkaline hydrolysis, methylation, TLC, autoradiography, and counting (29).
  • 37
    • 1842323767 scopus 로고    scopus 로고
    • note
    • We thank M. Sonnenberg for technical support, M. Hein for preparation of the manuscript, and C. Marander for graphics. Supported by grants AI-18357, AI-35220, and AI-38087 from the National Institute of Allergy and Infectious Diseases (NIAID), NIH, and SmithKline, Biochem., and contract AI-25147 from NIAID.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.