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note
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4 primers. This product was ligated into pET23b, as described for the nonmutated fbpC fragment, to yield the recombinant plasmid pCSB9sa. Fidelity of the pCSB9 and pCSB9sa constructs was determined by sequence analysis.
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1842401448
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14C-labeled, delipidated cells, rich in the bound mycolate esters of arabinogalactan, were subjected to alkaline hydrolysis, methylation, TLC, autoradiography, and counting (29).
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37
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1842323767
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note
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We thank M. Sonnenberg for technical support, M. Hein for preparation of the manuscript, and C. Marander for graphics. Supported by grants AI-18357, AI-35220, and AI-38087 from the National Institute of Allergy and Infectious Diseases (NIAID), NIH, and SmithKline, Biochem., and contract AI-25147 from NIAID.
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