Insights into transition state stabilization of the β-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants

Nature Structural Biology

Volumn 5, Issue 9, 1998, Pages 812-818

  Notenboom, Valerie ^a   Birsan, Camelia ^b   Nitz, Mark ^b   Rose, David R ^a   Warren, R Antony J ^b   Withers, Stephen G ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORINE; GLYCOSIDASE;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION; X RAY CRYSTALLOGRAPHY;

BETA-GLUCOSIDASE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; ELECTROSTATICS; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; GRAM-POSITIVE ASPOROGENOUS RODS, IRREGULAR; HISTIDINE; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; XYLOSIDASES;

EID: 0031715607     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/1852     Document Type: Article

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