Anti-estradiol-17β single-chain Fv fragments: Generation, characterization, gene randomization, and optimized phage display

Steroids

Volumn 73, Issue 14, 2008, Pages 1485-1499

  Kobayashi, Norihiro ^a   Kato, Yoshinori ^a   Oyama, Hiroyuki ^a   Taga, Shiori ^a   Niwa, Toshifumi ^b   Sun, Pi ^c   Ohtoyo, Mamoru ^c   Goto, Junichi ^c,d  

^a KOBE PHARMACEUTICAL UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c TOHOKU UNIVERSITY   (Japan)

^d TOHOKU UNIVERSITY HOSPITAL   (Japan)

Author keywords

Antibody engineering; Estradiol 17 ; Hapten; Phage display; Random mutagenesis; Single chain Fv fragment

Indexed keywords

17ALPHA ESTRADIOL; DNA FRAGMENT; ESTRADIOL; ESTRIOL; ESTRONE; ETHINYLESTRADIOL; HAPTEN; HYDROCORTISONE; N HYDROXYSUCCINIMIDE; PROGESTERONE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TESTOSTERONE; ESTROGEN; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN FV; MONOCLONAL ANTIBODY; PEPTIDE LIBRARY;

ANIMAL EXPERIMENT; ANTIBODY ENGINEERING; ARTICLE; BAGG ALBINO MOUSE; CELL FUSION; CHEMICAL STRUCTURE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE INSERTION; GENE LIBRARY; GENE MUTATION; HYBRIDOMA; MOUSE; MUTAGENESIS; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; RADIOIMMUNOASSAY; SPLEEN CELL; SURFACE PLASMON RESONANCE; AMINO ACID SEQUENCE; ANIMAL; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESTRADIOL; ESTROGENS; FEMALE; HYBRIDOMAS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 55549142494     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.steroids.2008.08.009     Document Type: Article

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