Functional characterization of an anti-estradiol antibody by site-directed mutagenesis and molecular modelling: Modulation of binding properties and prominent role of the VL domain in estradiol recognition

Journal of Molecular Recognition

Volumn 15, Issue 1, 2002, Pages 6-18

  Coulon, Stéphane ^a   Pellequer, Jean Luc ^b   Blachère, Thierry ^c   Chartier, Martine ^a   Mappus, Elisabeth ^c   Chen, Shu Wen W ^b   Cuilleron, Claude Yves ^c   Baty, Daniel ^a,d  

^a CNRS and Aix Marseille University   (France)

^b DSV/DIEP/SBTN   (France)

^c HÔPITAL DEBROUSSE   (France)

^d CNRS   (France)

Author keywords

Anti steroid; Antibody engineering; Estradiol; Molecular modelling; Photolabelling; Site directed mutagenesis

Indexed keywords

ESCHERICHIA COLI;

ANTIBODY; ESTRADIOL;

AMINO ACID SEQUENCE; ANIMAL; ANTIBODY AFFINITY; ANTIBODY COMBINING SITE; ANTIBODY SPECIFICITY; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; IMMUNOLOGY; MOLECULAR GENETICS; MOUSE; PHOTOAFFINITY LABELING; PROTEIN ENGINEERING; RADIOASSAY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; BINDING SITES, ANTIBODY; ESTRADIOL; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOTOAFFINITY LABELS; PROTEIN ENGINEERING; RADIOLIGAND ASSAY;

EID: 0036006146     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmr.553     Document Type: Article

References (45)

1. Three-dimensional structure of an anti-steroid Fab' and progesterone-Fab' complex
2. Structural analysis of antibody specificity. Detailed comparison of five Fab'-steroid complexes
3. Small rearrangements in structures of Fv and Fab fragments of antibody D1.3 on antigen binding
4. Three-dimensional structures of the free and the antigen-complexed Fab from monoclonal anti-lysozyme antibody D44.1
5. CHARMM: A program for macromolecular energy, minimization, and molecular dynamics calculations
6. Improving the affinity and the fine specificity of an anti-cortisol antibody by parsimonious mutagenesis and phage display
7. Intracellular immunization of prokaryotic cells against a bacteriotoxin
8. Selection and analysis of an optimized anti-VEGF antibody: Crystal structure of an affinity-matured Fab in complex with antigen
9. Canonical structures for the hypervariable regions of immunoglobulins
10. Conformations of immunoglobulin hypervariable regions
11. Who checks the checkers? Four validation tools applied to eight atomic resolution structures
12. Monoclonal antibodies against progesterone: Effect of steroid-carrier coupling position on antibody specificity
13. A novel HPLC-RIA method for the simultaneous detection of estrone, estradiol and estrone sulphate levels in breast cancer tissue
14. Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains
15. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
16. 26-10 Fab-digoxin complex: Affinity and specificity due to surface complementarity
17. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
18. Structural analysis of an anti-estradiol antibody
19. Expanding the conformational diversity by random insertions to CDRH2 results in improved anti-estradiol antibodies
20. Crystal structure of a recombinant anti-estradiol Fab fragment in complex with 17 β-estradiol
21. PROCHECK: A program to check the stereochemical quality of protein structures
22. Paratope characterization by structural modelling of two anti-cortisol single-chain variable fragments produced in E. coli
23. Aromatic rings act as hydrogen bond acceptors
24. Antibody-antigen interactions: Contact analysis and binding site topography
25. By-passing immunization: Building high affinity human antibodies by chain shuffling
26. XtalView: A visual protein crystallographic software system for XII/Xview
27. Raster3D: Photorealistic molecular graphics
28. Anatomy of the antibody molecule
29. H interface upon antigen binding
30. Antibody engineering: Advances from the use of Escherichia coli expression systems
31. Catalytic antibody model and mutagenesis implicate arginine in transition-state stabilization
32. Specific photoaffinity labelling of Tyr-50 on the heavy chain and of Tyr-32 on the light chain in the steroid combining site of a mouse monoclonal anti-estradiol antibody using C3-, C6-, and C7-linked 5-azido-2-nitrobenzoylamidoestradiol photoreagents
33. Turbo-Frodo
34. Specific photoaffinity labelling of Tyr-49 on the light chain in the steroid-combining site of a mouse monoclonal anti-estradiol antibody using two epimeric 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amidoestradiol photoreagents
35. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli
36. Cleavage specificity of a proteolytic antibody light chain and effects of the heavy chain variable domain
37. Light chain determines the binding property of human: Anti-dsDNA lgG autoantibodies
38. Antibody fragment Fv4155 bound to two closely related steroid hormones: The structural basis of fine specificity
39. A new approach to the rapid determination of protein side chain conformations
40. Crystal structures of the free and liganded form of an esterolytic catalytic antibody
41. An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability
42. Influence of the conjugation site on the specificity of monoclonal antibodies to progesterone and on the performance of direct enzyme immunoassay