Modulating the binding properties of an anti-17β-estradiol antibody by systematic mutation combinations

Protein Science

Volumn 12, Issue 11, 2003, Pages 2549-2558

  Lamminmäki, Urpo ^a   Westerlund Karlsson, Annette ^a,b   Toivola, Maria ^a   Saviranta, Petri ^a,c  

^a UNIVERSITY OF TURKU   (Finland)

^b ÅBO AKADEMI UNIVERSITY   (Finland)

^c GENOMICS INSTITUTE OF THE NOVARTIS RESEARCH FOUNDATION   (United States)

Author keywords

Antibody; Estradiol; Mutation; Site directed mutagenesis; Steroid

Indexed keywords

ESTRADIOL ANTIBODY; ESTRONE; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; TESTOSTERONE;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BINDING KINETICS; CORRELATION ANALYSIS; CROSS REACTION; CRYSTAL STRUCTURE; DISSOCIATION CONSTANT; MODULATION; MOLECULAR INTERACTION; MOLECULAR MODEL; PHAGE DISPLAY; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

ANTIBODIES; ANTIBODY SPECIFICITY; BINDING SITES, ANTIBODY; CLONING, MOLECULAR; ESCHERICHIA COLI; ESTRADIOL; IMMUNOGLOBULIN VARIABLE REGION; KINETICS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTATION; PROTEIN CONFORMATION; STEROIDS;

INSERTION SEQUENCES;

EID: 0142179040     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0353903     Document Type: Article

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