Exploiting sequence space: Shuffling in vivo formed complementarity determining regions into a master framework

Gene

Volumn 215, Issue 2, 1998, Pages 471-476

  Jirholt, P ^a   Ohlin, M ^a   Borrebaeck, C A K ^a   Soderlind E ^a,b  

^a LUND UNIVERSITY   (Sweden)

^b BioInvent International AB   (Sweden)

Author keywords

CDR shuffling; Gene assembly; Immunoglobulin germline gene; Primer design; Synthetic gene library

Indexed keywords

ARTICLE; COMPLEMENTARITY DETERMINING REGION; GENE LIBRARY; GENE SEQUENCE; GERM LINE; HUMAN; HUMAN CELL; IMMUNOGLOBULIN GENE; PRIORITY JOURNAL; TECHNIQUE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ESCHERICHIA COLI; GENE LIBRARY; GENES, IMMUNOGLOBULIN; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; OLIGODEOXYRIBONUCLEOTIDES; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032581610     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(98)00317-5     Document Type: Article

References (22)

1. Barbas III, C.F., Bain, J.D., Hoekstra, D.M., Lerner, R.A., 1992. Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem. Proc. Natl. Acad. Sci. USA 89, 4457-4461.
2. + B cells. 1997. J. Clin. Invest. 99, 2488-2501.
3. Cook, G.P., Tomlinson, I.M., 1995. The human immunoglobulin VH repertoire. Immunol. Today 16, 237-242.
4. Crameri, A., Stemmer, W.P., 1995. Combinatorial multiple cassette mutagenesis creates all the permutations of mutant and wild-type sequences. Biotechniques 18, 194-196.
5. Davies, J., Riechmann, L., 1995. Antibody VH domains as small recognition units. Bio/Technology 13, 475-479.
6. Griffiths, A.D., Williams, S.C., Hartley, O., Tomlinson, I.M., Waterhouse, P., Crosby, W.L., Kontermann, R.E., Jones, P.T., Low, N.M., Allison, T.J., Hoogenboom, H.R., Nissim, A., Cox, J.P.L., Harrison, J.L., Zaccolo, M., Gherardi, E., Winter, G., 1994. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13, 3245-3260.
7. Hayashi, N., Welschof, M., Zewe, M., Braunagel, M., Dübel, S., Breitling, F., Little, M., 1994. Simultaneous mutagenesis of antibody CDR regions by overlap extension and PCR. Biotechniques 17, 310-315.
8. Hayden, M.S., Gilliland, L.K., Ledbetter, J.A., 1997. Antibody engineering. Curr. Opin. Immunol. 9, 201-212.
9. Hoogenboom, H.R., Winter, G., 1992. By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro. J. Mol. Biol. 20, 381-388.
10. Huang, S.C., Jiang, R., Glas, A.M., Milner, E.C.B., 1996. Non-stochastic utilization of Ig V-region genes in unselected human peripheral B cells. Mol. Immunol. 33, 553-560.
11. Kabat, E.A., Wu, T.T., Perry, H.M., Gottesman, K.S., Foeller, C., 1991. Sequences of Immunological Interest, 5th ed. NIH Publication No. 91-3242. National Institutes of Public Health, Bethesda, MD.
12. Klein, U., Küppers, R., Rajewsky, K., 1997. Evidence for a large compartment of IgM-expressing memory B cells in humans. Blood 89, 1288-1293.
13. Kobayashi, N., Söderlind, E., Borrebaeck, C.A.K., 1997. Analysis of assembly of synthetic antibody fragments: expression of functional scFv with predefined specificity. Biotechniques 23, 500-503.
14. Ohlin, M., Borrebaeck, C.A.K., 1996. Characteristics of human antibody repertoires following active immune responses in vivo. Mol. Immunol. 33, 583-592.
15. Ohlin, M., Owman, H., Mach, M., Borrebaeck, C.A.K., 1996. Light chain shuffling of a high affinity antibody results in a drift in epitope recognition. Mol. Immunol. 33, 47-56.
16. Plückthun, A., Pack, P., 1997. New protein engineering approaches to multivalent and bispecific antibody fragments. Immunotechnology 3, 83-105.
17. Stemmer, W.P., 1994. Rapid evolution of a protein in vitro by DNA shuffling. Nature 370, 389-391.
18. Söderlind, E., Vergeles, M., Borrebaeck, C.A.K., 1995. Domain libraries: synthetic diversity for de novo design of antibody V-regions. Gene 160, 269-272.
19. Tindall, K.R., Kunkel, T.A., 1988. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Biochemistry 27, 6008-6013.
20. Virnekas, B., Ge, L., Plückthun, A., Schneider, K.C., Wellnhofer, G., Moroney, S.E., 1994. Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. Nucleic Acids Res. 22, 5600-5607.
21. Wu, T.T., Johnsson, G., Kabat, E.A., 1993. Length distribution of CDRH3 in antibodies. Proteins 16, 1-7.
22. Zhao, H., Giver, L., Shao, Z., Affholter, J.A., Arnold, F.H., 1998. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat. Biotechnol. 16, 258-261.