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Volumn 182, Issue 4, 2008, Pages 715-726

Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles

Author keywords

[No Author keywords available]

Indexed keywords

DYNACTIN; DYNEIN ADENOSINE TRIPHOSPHATASE; MOLECULAR MOTOR; PROTEIN EG5;

EID: 50249103819     PISSN: 00219525     EISSN: 00219525     Source Type: Journal    
DOI: 10.1083/jcb.200801125     Document Type: Article
Times cited : (77)

References (58)
  • 2
    • 0029417238 scopus 로고
    • Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo
    • Blangy, A., H.A. Lane, P. d'Herin, M. Harper, M. Kress, and E.A. Nigg. 1995. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell. 83:1159-1169.
    • (1995) Cell , vol.83 , pp. 1159-1169
    • Blangy, A.1    Lane, H.A.2    d'Herin, P.3    Harper, M.4    Kress, M.5    Nigg, E.A.6
  • 3
    • 0030612145 scopus 로고    scopus 로고
    • Phosphorylation by p34cdc2 protein kinase regulates binding of the kinesin-related motor HsEg5 to the dynactin subunit p150
    • Blangy, A., L. Arnaud, and E.A. Nigg. 1997. Phosphorylation by p34cdc2 protein kinase regulates binding of the kinesin-related motor HsEg5 to the dynactin subunit p150. J. Biol. Chem. 272:19418-19424.
    • (1997) J. Biol. Chem , vol.272 , pp. 19418-19424
    • Blangy, A.1    Arnaud, L.2    Nigg, E.A.3
  • 4
    • 33750693006 scopus 로고    scopus 로고
    • A new method reveals microtubule minus ends throughout the meiotic spindle
    • Burbank, K.S., A.C. Groen, Z.E. Perlman, D.S. Fisher, and T.J. Mitchison. 2006. A new method reveals microtubule minus ends throughout the meiotic spindle. J. Cell Biol. 175:369-375.
    • (2006) J. Cell Biol , vol.175 , pp. 369-375
    • Burbank, K.S.1    Groen, A.C.2    Perlman, Z.E.3    Fisher, D.S.4    Mitchison, T.J.5
  • 5
    • 34547858506 scopus 로고    scopus 로고
    • Slide-and-cluster models for spindle assembly
    • Burbank, K.S., T.J. Mitchison, and D.S. Fisher. 2007. Slide-and-cluster models for spindle assembly. Curr. Biol. 17:1373-1383.
    • (2007) Curr. Biol , vol.17 , pp. 1373-1383
    • Burbank, K.S.1    Mitchison, T.J.2    Fisher, D.S.3
  • 7
    • 0345393105 scopus 로고    scopus 로고
    • Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer
    • Castoldi, M., and A.V. Popov. 2003. Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer. Protein Expr. Purif. 32:83-88.
    • (2003) Protein Expr. Purif , vol.32 , pp. 83-88
    • Castoldi, M.1    Popov, A.V.2
  • 8
    • 0028168413 scopus 로고
    • A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos
    • Cole, D.G., W.M. Saxton, K.B. Sheehan, and J.M. Scholey. 1994. A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos. J. Biol. Chem. 269:22913-22916.
    • (1994) J. Biol. Chem , vol.269 , pp. 22913-22916
    • Cole, D.G.1    Saxton, W.M.2    Sheehan, K.B.3    Scholey, J.M.4
  • 9
    • 3242776257 scopus 로고    scopus 로고
    • The kinetic mechanism of kinesin
    • Cross, R.A. 2004. The kinetic mechanism of kinesin. Trends Biochem. Sci. 29:301-309.
    • (2004) Trends Biochem. Sci , vol.29 , pp. 301-309
    • Cross, R.A.1
  • 10
    • 0032482236 scopus 로고    scopus 로고
    • Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles
    • Desai, A., P.S. Maddox, T.J. Mitchison, and E.D. Salmon. 1998. Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles. J. Cell Biol. 141:703-713.
    • (1998) J. Cell Biol , vol.141 , pp. 703-713
    • Desai, A.1    Maddox, P.S.2    Mitchison, T.J.3    Salmon, E.D.4
  • 11
    • 0027391206 scopus 로고
    • Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe
    • Ding, R., K.L. McDonald, and J.R. McIntosh. 1993. Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe. J. Cell Biol. 120: 141-151.
    • (1993) J. Cell Biol , vol.120 , pp. 141-151
    • Ding, R.1    McDonald, K.L.2    McIntosh, J.R.3
  • 12
    • 4544315452 scopus 로고    scopus 로고
    • Mechanisms and molecules of the mitotic spindle
    • Gadde, S., and R. Heald. 2004. Mechanisms and molecules of the mitotic spindle. Curr. Biol. 14:R797-R805.
    • (2004) Curr. Biol , vol.14
    • Gadde, S.1    Heald, R.2
  • 13
    • 4143127831 scopus 로고    scopus 로고
    • Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles
    • Gaetz, J., and T.M. Kapoor. 2004. Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles. J. Cell Biol. 166:465-471.
    • (2004) J. Cell Biol , vol.166 , pp. 465-471
    • Gaetz, J.1    Kapoor, T.M.2
  • 14
  • 15
    • 0141768258 scopus 로고    scopus 로고
    • The roles of microtubule-based motor proteins in mitosis: Comprehensive RNAi analysis in the Drosophila S2 cell line
    • Goshima, G., and R.D. Vale. 2003. The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line. J. Cell Biol. 162:1003-1016
    • (2003) J. Cell Biol , vol.162 , pp. 1003-1016
    • Goshima, G.1    Vale, R.D.2
  • 16
    • 0026577748 scopus 로고
    • Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast
    • Hagan, I., and M. Yanagida. 1992. Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast. Nature. 356:74-76.
    • (1992) Nature , vol.356 , pp. 74-76
    • Hagan, I.1    Yanagida, M.2
  • 17
    • 0032528290 scopus 로고    scopus 로고
    • MPF localization is controlled by nuclear export
    • Hagting, A., C. Karlsson, P. Clute, M. Jackman, and J. Pines. 1998. MPF localization is controlled by nuclear export. EMBO J. 17:4127-4138.
    • (1998) EMBO J , vol.17 , pp. 4127-4138
    • Hagting, A.1    Karlsson, C.2    Clute, P.3    Jackman, M.4    Pines, J.5
  • 18
    • 34548835462 scopus 로고    scopus 로고
    • Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts
    • Hannak, E., and R. Heald. 2006. Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts. Nat. Protoc. 1:2305-2314.
    • (2006) Nat. Protoc , vol.1 , pp. 2305-2314
    • Hannak, E.1    Heald, R.2
  • 19
    • 0030751640 scopus 로고    scopus 로고
    • Spindle assembly in Xenopus egg extracts: Respective roles of centrosomes and microtubule self-organization
    • Heald, R., R. Tournebize, A. Habermann, E. Karsenti, and A. Hyman. 1997. Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization. J. Cell Biol. 138:615-628.
    • (1997) J. Cell Biol , vol.138 , pp. 615-628
    • Heald, R.1    Tournebize, R.2    Habermann, A.3    Karsenti, E.4    Hyman, A.5
  • 20
    • 0034677736 scopus 로고    scopus 로고
    • Mitotic motors in Saccharomyces cerevisiae
    • Hildebrandt, E.R., and M.A. Hoyt. 2000. Mitotic motors in Saccharomyces cerevisiae. Biochim. Biophys. Acta. 1496:99-116.
    • (2000) Biochim. Biophys. Acta , vol.1496 , pp. 99-116
    • Hildebrandt, E.R.1    Hoyt, M.A.2
  • 22
    • 18344371892 scopus 로고    scopus 로고
    • The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks
    • Kapitein, L.C., E.J. Peterman, B.H. Kwok, J.H. Kim, T.M. Kapoor, and C.F. Schmidt. 2005. The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks. Nature. 435:114-118.
    • (2005) Nature , vol.435 , pp. 114-118
    • Kapitein, L.C.1    Peterman, E.J.2    Kwok, B.H.3    Kim, J.H.4    Kapoor, T.M.5    Schmidt, C.F.6
  • 23
    • 0035904233 scopus 로고    scopus 로고
    • Eg5 is static in bipolar spindles relative to tubulin: Evidence for a static spindle matrix
    • Kapoor, T.M., and T.J. Mitchison. 2001. Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix. J. Cell Biol. 154:1125-1133.
    • (2001) J. Cell Biol , vol.154 , pp. 1125-1133
    • Kapoor, T.M.1    Mitchison, T.J.2
  • 25
    • 0033789351 scopus 로고    scopus 로고
    • Dynactin increases the processivity of the cytoplasmic dynein motor
    • King, S.J., and T.A. Schroer. 2000. Dynactin increases the processivity of the cytoplasmic dynein motor. Nat. Cell Biol. 2:20-24.
    • (2000) Nat. Cell Biol , vol.2 , pp. 20-24
    • King, S.J.1    Schroer, T.A.2
  • 27
    • 13844263242 scopus 로고    scopus 로고
    • Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush
    • Lata, S., and J. Piehler. 2005. Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush. Anal. Chem. 77:1096-1105.
    • (2005) Anal. Chem , vol.77 , pp. 1096-1105
    • Lata, S.1    Piehler, J.2
  • 29
    • 0042733309 scopus 로고    scopus 로고
    • Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: Implications for spindle mechanics
    • Maddox, P., A. Straight, P. Coughlin, T.J. Mitchison, and E.D. Salmon. 2003. Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics. J. Cell Biol. 162:377-382.
    • (2003) J. Cell Biol , vol.162 , pp. 377-382
    • Maddox, P.1    Straight, A.2    Coughlin, P.3    Mitchison, T.J.4    Salmon, E.D.5
  • 31
    • 0033615357 scopus 로고    scopus 로고
    • Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen
    • Mayer, T.U., T.M. Kapoor, S.J. Haggarty, R.W. King, S.L. Schreiber, and T.J. Mitchison. 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science. 286:971-974.
    • (1999) Science , vol.286 , pp. 971-974
    • Mayer, T.U.1    Kapoor, T.M.2    Haggarty, S.J.3    King, R.W.4    Schreiber, S.L.5    Mitchison, T.J.6
  • 32
    • 0021368204 scopus 로고
    • Tubulin hooks as probes for microtubule polarity: An analysis of the method and an evaluation of data on microtubule polarity in the mitotic spindle
    • McIntosh, J.R., and U. Euteneuer. 1984. Tubulin hooks as probes for microtubule polarity: an analysis of the method and an evaluation of data on microtubule polarity in the mitotic spindle. J. Cell Biol. 98:525-533.
    • (1984) J. Cell Biol , vol.98 , pp. 525-533
    • McIntosh, J.R.1    Euteneuer, U.2
  • 34
    • 24344465919 scopus 로고    scopus 로고
    • Analysis of the kinesin superfamily: Insights into structure and function
    • Miki, H., Y. Okada, and N. Hirokawa. 2005. Analysis of the kinesin superfamily: insights into structure and function. Trends Cell Biol. 15:467-476.
    • (2005) Trends Cell Biol , vol.15 , pp. 467-476
    • Miki, H.1    Okada, Y.2    Hirokawa, N.3
  • 35
    • 0021686169 scopus 로고
    • Dynamic instability of microtubule growth
    • Mitchison, T., and M. Kirschner. 1984. Dynamic instability of microtubule growth. Nature. 312: 237-242.
    • (1984) Nature , vol.312 , pp. 237-242
    • Mitchison, T.1    Kirschner, M.2
  • 36
    • 19644392512 scopus 로고    scopus 로고
    • Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles
    • Mitchison, T.J., P. Maddox, J. Gaetz, A. Groen, M. Shirasu, A. Desai, E.D. Salmon, and T.M. Kapoor. 2005. Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles. Mol. Biol. Cell. 16:3064-3076.
    • (2005) Mol. Biol. Cell , vol.16 , pp. 3064-3076
    • Mitchison, T.J.1    Maddox, P.2    Gaetz, J.3    Groen, A.4    Shirasu, M.5    Desai, A.6    Salmon, E.D.7    Kapoor, T.M.8
  • 37
    • 9444245327 scopus 로고    scopus 로고
    • The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles
    • Miyamoto, D.T., Z.E. Perlman, K.S. Burbank, A.C. Groen, and T.J. Mitchison. 2004. The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles. J. Cell Biol. 167:813-818.
    • (2004) J. Cell Biol , vol.167 , pp. 813-818
    • Miyamoto, D.T.1    Perlman, Z.E.2    Burbank, K.S.3    Groen, A.C.4    Mitchison, T.J.5
  • 38
    • 12344266732 scopus 로고    scopus 로고
    • Recent progress in dynein structure and mechanism
    • Oiwa, K., and H. Sakakibara. 2005. Recent progress in dynein structure and mechanism. Curr. Opin. Cell Biol. 17:98-103.
    • (2005) Curr. Opin. Cell Biol , vol.17 , pp. 98-103
    • Oiwa, K.1    Sakakibara, H.2
  • 39
    • 0037072602 scopus 로고    scopus 로고
    • A photoactivatable GFP for selective photolabeling of proteins and cells
    • Patterson, G.H., and J. Lippincott-Schwartz. 2002. A photoactivatable GFP for selective photolabeling of proteins and cells. Science. 297:1873-1877.
    • (2002) Science , vol.297 , pp. 1873-1877
    • Patterson, G.H.1    Lippincott-Schwartz, J.2
  • 41
    • 0026015997 scopus 로고
    • Poleward microtubule flux mitotic spindles assembled in vitro
    • Sawin, K.E., and T.J. Mitchison. 1991. Poleward microtubule flux mitotic spindles assembled in vitro. J. Cell Biol. 112:941-954.
    • (1991) J. Cell Biol , vol.112 , pp. 941-954
    • Sawin, K.E.1    Mitchison, T.J.2
  • 42
    • 0026739078 scopus 로고
    • Mitotic spindle organization by a plus-end-directed microtubule motor
    • Sawin, K.E., K. LeGuellec, M. Philippe, and T.J. Mitchison. 1992. Mitotic spindle organization by a plus-end-directed microtubule motor. Nature. 359:540-543.
    • (1992) Nature , vol.359 , pp. 540-543
    • Sawin, K.E.1    LeGuellec, K.2    Philippe, M.3    Mitchison, T.J.4
  • 44
    • 31444437864 scopus 로고    scopus 로고
    • Processive movement of single kinesins on crowded microtubules visualized using quantum dots
    • Seitz, A., and T. Surrey. 2006. Processive movement of single kinesins on crowded microtubules visualized using quantum dots. EMBO J. 25:267-277.
    • (2006) EMBO J , vol.25 , pp. 267-277
    • Seitz, A.1    Surrey, T.2
  • 45
    • 0033545186 scopus 로고    scopus 로고
    • The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles
    • Sharp, D.J., K.L. McDonald, H.M. Brown, H.J. Matthies, C. Walczak, R.D. Vale, T.J. Mitchison, and J.M. Scholey. 1999a. The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles. J. Cell Biol. 144:125-138.
    • (1999) J. Cell Biol , vol.144 , pp. 125-138
    • Sharp, D.J.1    McDonald, K.L.2    Brown, H.M.3    Matthies, H.J.4    Walczak, C.5    Vale, R.D.6    Mitchison, T.J.7    Scholey, J.M.8
  • 46
    • 0033126118 scopus 로고    scopus 로고
    • Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos
    • Sharp, D.J., K.R. Yu, J.C. Sisson, W. Sullivan, and J.M. Scholey. 1999b. Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos. Nat. Cell Biol. 1:51-54.
    • (1999) Nat. Cell Biol , vol.1 , pp. 51-54
    • Sharp, D.J.1    Yu, K.R.2    Sisson, J.C.3    Sullivan, W.4    Scholey, J.M.5
  • 48
    • 13244291467 scopus 로고    scopus 로고
    • FRAP analysis of binding: Proper and fitting
    • Sprague, B.L., and J.G. McNally. 2005. FRAP analysis of binding: proper and fitting. Trends Cell Biol. 15:84-91.
    • (2005) Trends Cell Biol , vol.15 , pp. 84-91
    • Sprague, B.L.1    McNally, J.G.2
  • 49
    • 0019426542 scopus 로고
    • Decoration of spindle microtubules with Dynein: Evidence for uniform polarity
    • Telzer, B.R., and L.T. Haimo. 1981. Decoration of spindle microtubules with Dynein: evidence for uniform polarity. J. Cell Biol. 89:373-378.
    • (1981) J. Cell Biol , vol.89 , pp. 373-378
    • Telzer, B.R.1    Haimo, L.T.2
  • 50
    • 1642546280 scopus 로고    scopus 로고
    • Microtubule plus-end dynamics in Xenopus egg extract spindles
    • Tirnauer, J.S., E.D. Salmon, and T.J. Mitchison. 2004. Microtubule plus-end dynamics in Xenopus egg extract spindles. Mol. Biol. Cell. 15:1776-1784.
    • (2004) Mol. Biol. Cell , vol.15 , pp. 1776-1784
    • Tirnauer, J.S.1    Salmon, E.D.2    Mitchison, T.J.3
  • 51
    • 0343415156 scopus 로고    scopus 로고
    • The way things move: Looking under the hood of molecular motor proteins
    • Vale, R.D., and R.A. Milligan. 2000. The way things move: looking under the hood of molecular motor proteins. Science. 288:88-95.
    • (2000) Science , vol.288 , pp. 88-95
    • Vale, R.D.1    Milligan, R.A.2
  • 52
    • 33744987629 scopus 로고    scopus 로고
    • Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro
    • Valentine, M.T., P.M. Fordyce, T.C. Krzysiak, S.P. Gilbert, and S.M. Block. 2006. Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro. Nat. Cell Biol. 8:470-476.
    • (2006) Nat. Cell Biol , vol.8 , pp. 470-476
    • Valentine, M.T.1    Fordyce, P.M.2    Krzysiak, T.C.3    Gilbert, S.P.4    Block, S.M.5
  • 53
    • 13144251122 scopus 로고    scopus 로고
    • A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity
    • Walczak, C.E., I. Vernos, T.J. Mitchison, E. Karsenti, and R. Heald. 1998. A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity. Curr. Biol. 8:903-913.
    • (1998) Curr. Biol , vol.8 , pp. 903-913
    • Walczak, C.E.1    Vernos, I.2    Mitchison, T.J.3    Karsenti, E.4    Heald, R.5
  • 54
    • 0032607419 scopus 로고    scopus 로고
    • Fluorescent speckle microscopy of spindle microtubule assembly and motility in living cells
    • Waterman-Storer, C., A. Desai, and E.D. Salmon. 1999. Fluorescent speckle microscopy of spindle microtubule assembly and motility in living cells. Methods Cell Biol. 61:155-173.
    • (1999) Methods Cell Biol , vol.61 , pp. 155-173
    • Waterman-Storer, C.1    Desai, A.2    Salmon, E.D.3
  • 55
    • 0037125918 scopus 로고    scopus 로고
    • New directions for fluorescent speckle microscopy
    • Waterman-Storer, C.M., and G. Danuser. 2002. New directions for fluorescent speckle microscopy. Curr. Biol. 12:R633-R640.
    • (2002) Curr. Biol , vol.12
    • Waterman-Storer, C.M.1    Danuser, G.2
  • 56
    • 0032622374 scopus 로고    scopus 로고
    • Recombinant p50/dynamitin as a tool to examine the role of dynactin in intracellular processes
    • Wittmann, T., and T. H yman. 1999. Recombinant p50/dynamitin as a tool to examine the role of dynactin in intracellular processes. Methods Cell Biol. 61:137-143.
    • (1999) Methods Cell Biol , vol.61 , pp. 137-143
    • Wittmann, T.1    yman, T.H.2
  • 57
    • 0035146128 scopus 로고    scopus 로고
    • The spindle: A dynamic assembly of microtubules and motors
    • Wittmann, T., A. Hyman, and A. Desai. 2001. The spindle: a dynamic assembly of microtubules and motors. Nat. Cell Biol. 3:E28-E34.
    • (2001) Nat. Cell Biol , vol.3
    • Wittmann, T.1    Hyman, A.2    Desai, A.3
  • 58
    • 35748939386 scopus 로고    scopus 로고
    • Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging
    • Yang, G., B.R. Houghtaling, J. Gaetz, J.Z. Liu, G. Danuser, and T.M. Kapoor. 2007. Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging. Nat. Cell Biol. 9:1233-1242.
    • (2007) Nat. Cell Biol , vol.9 , pp. 1233-1242
    • Yang, G.1    Houghtaling, B.R.2    Gaetz, J.3    Liu, J.Z.4    Danuser, G.5    Kapoor, T.M.6


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