The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles

Journal of Cell Biology

Volumn 167, Issue 5, 2004, Pages 813-818

  Miyamoto, David T ^a   Perlman, Zachary E ^a   Burbank, Kendra S ^a,b   Groen, Aaron C ^a   Mitchison, Timothy J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KINESIN; KINESIN EG5; MONASTROL; PROTEIN INHIBITOR; QUINAZOLINE; QUINAZOLINONE DERIVATIVE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; AUTOMATION; DOSE RESPONSE; FLUORESCENCE MICROSCOPY; MEASUREMENT; MICROTUBULE; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; XENOPUS LAEVIS;

ANIMALS; CELL DIVISION; CELL EXTRACTS; CELL POLARITY; CHROMOSOME SEGREGATION; CYTOKINESIS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; FEMALE; IMAGE CYTOMETRY; KINESIN; METAPHASE; MICROSCOPY, FLUORESCENCE; MICROTUBULES; MITOTIC SPINDLE APPARATUS; MOLECULAR MOTOR PROTEINS; OOCYTES; PYRIMIDINES; SUBCELLULAR FRACTIONS; THIONES; XENOPUS LAEVIS; XENOPUS PROTEINS;

ANIMALIA; VERTEBRATA; XENOPUS LAEVIS;

EID: 9444245327     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200407126     Document Type: Article

References (30)

1. Brust-Mascher, I., and J.M. Scholey. 2002. Microtubule flux and sliding in mitotic spindles of Drosophila embryos. Mol. Biol. Cell. 13:3967-3975.
2. Chen, W., and D. Zhang. 2004. Kinetochore fibre dynamics outside the context of the spindle during anaphase. Nat. Cell Biol. 6:227-231.
3. Crevel, I.M., A. Lockhart, and R.A. Cross. 1997. Kinetic evidence for low chemical processivity in ncd and Eg5. J. Mol. Biol. 273:160-170.
4. Crevel, I.M., M.C. Alonso. and R.A. Cross. 2004. Monastrol stabilises an attached low-friction mode of Eg5. Curr. Biol. 14:R411-R412.
5. Desai, A., P.S. Maddox, T.J. Mitchison, and E.D. Salmon. 1998. Anaphase A chromosome movement and poleward spindle microtubule flux occur at similar rates in Xenopus extract spindles. J. Cell Biol. 141:703-713.
6. Desai, A., A. Murray, T.J. Mitchison, and C.E. Walczak. 1999. The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro. Methods Cell Biol. 61:385-412.
7. Enos, A.P., and N.R. Morris. 1990. Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans. Cell 60:1019-1027.
8. Finer, J.T., G. Bergnes, B. Feng, W.W. Smith, and J.C. Chabala. 2001. Methods and compositions using quinazolinones. In World Intellectual Property Organization. Vol. WO 01/30768 A. http://www.wipo.int/pct/en/ (accessed July 19, 2004.
9. Hagan, I., and M. Yanagida. 1992. Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast. Nature. 356:74-76.
10. Hotha, S., J.C. Yarrow, J.G. Yang, S. Garrett, K.V. Renduchintala, T.U. Mayer, and T.M. Kapoor. 2003. HR22C16: a potent small-molecule probe for the dynamics of cell division. Angew. Chem. Int. Ed. Engl. 42:2379-2382.
11. Howard, J. 2001. Speeds of motors. In Mechanics of Motor Proteins and the Cytoskeleton. Sinauer Associates, Inc., Sunderland, MA. 213-227.
12. Kapoor, T.M., and T.J. Mitchison. 2001. Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix. J. Cell Biol. 154:1125-1133.
13. Kapoor, T.M., T.U. Mayer, M.L. Coughlin, and T.J. Mitchison. 2000. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J. Cell Biol. 150:975-988.
14. Kashina, A.S., R.J. Baskin, D.O. Cole, K.P. Wedaman, W.M. Saxton, and J.M. Scholey. 1996. A bipolar kinesin. Nature. 379:270-272.
15. Maddox, P., A. Desai, K. Oegema, T.J. Mitchison, and E.D. Salmon. 2002. Poleward microtubule flux is a major component of spindle dynamics and anaphase a in mitotic Drosophila embryos. Curr. Biol. 12:1670-1674.
16. Maddox, P., A. Straight, P. Coughlin, T.J. Mitchison, and E.D. Salmon. 2003. Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics. J. Cell Biol 162:377-382.
17. Margolis, R.L., L. Wilson, and B.I. Keifer. 1978. Mitotic mechanism based on intrinsic microtubule behaviour. Nature. 272:450-452.
18. Mayer, T.U., T.M. Kapoor, S.J. Haggarty, R.W. King, S.L. Schreiber, and T.J. Mitchison. 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science. 286:971-974.
19. Mitchison, T.J. 1989. Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J. Cell Biol. 109:637-652.
20. Mitchison, T.J., and E.D. Salmon. 1992. Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis. J. Cell Biol. 119:569-582.
21. Mitchison, T.J., P. Maddox, A. Groen, L. Cameron, Z. Perlman, R. Ohi, A. Desai, E.D. Salmon, and T.M. Kapoor. 2004. Bipolarization and poleward flux correlate during Xenopus extract spindle assembly. Mol. Biol. Cell. doi:10.1091/mbc.E04-05-0440.
22. Rogers, G.C., S.L. Rogers, T.A. Schwimmer, S.C. Ems-McClung, C.E. Walczak, R.D. Vale, J.M. Scholey, and D.J. Sharp. 2004. Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature. 427:364-370.
23. Sawin, K.E., and T.J. Mitchison. 1991. Poleward microtubule flux mitotic spindles assembled in vitro. J. Cell Biol. 112:941-954.
24. Sawin, K.E., and T.J. Mitchison. 1994. Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules. Mol. Biol. Cell. 5:217-226.
25. Sawin, K.E., K. LeGuellec, M. Philippe, and T.J. Mitchison. 1992. Mitotic spindle organization by a plus-end-directed microtubule motor. Nature. 359:540-543.
26. Shirasu-Hiza, M., Z.E. Perlman, T. Wittmann, E. Karsenti, and T.J. Mitchison. 2004. Eg5 causes elongation of meiotic spindles when flux-associated microtubule depolymerization is blocked. Curr. Biol. 14:1941-1945.
27. Vallotton, P., A. Pond, C.M. Waterman-Storer, E.D. Salmon, and G. Danuser. 2003. Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study. Biophys. J. 85:1289-1306.
28. Waterman-Storer, C.M., A. Desai, J.C. Bulinski, and E.D. Salmon. 1998. Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Curr. Biol. 8:1227-1230.
29. Westerweel, J. 1997. Fundamentals of digital particle image velocimetry. Meas. Sci. Technol. 8:1379-1392.
30. Wittmann, T., and T. Hyman. 1999. Recombinant p50/dynamitin as a tool to examine the role of dynactin in intracellular processes. Methods Cell Biol. 61:137-143.