Eg5 is static in bipolar spindles relative to tubulin: Evidence for a static spindle matrix

Journal of Cell Biology

Volumn 154, Issue 6, 2001, Pages 1125-1133

  Kapoor, Tarun M ^a   Mitchison, Timothy J ^b  

^a ROCKEFELLER UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Eg5; Kinesin; Mitosis; Speckle; Tubulin

Indexed keywords

DYNACTIN; KINESIN; MATRIX PROTEIN; PROTEIN EG5; PROTEIN P50; PYROPHOSPHORIC ACID DERIVATIVE; TUBULIN; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CELL MOTILITY; CELL POLARITY; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; MICROTUBULE; MITOSIS SPINDLE; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; XENOPUS;

ADENOSINE TRIPHOSPHATASES; ADENYLYL IMIDODIPHOSPHATE; ANIMALS; CYTOPLASM; HUMANS; KINESIN; KINETICS; MICROSCOPY, FLUORESCENCE; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MITOTIC SPINDLE APPARATUS; MOLECULAR MOTOR PROTEINS; MOVEMENT; PYRIMIDINES; THIONES; XENOPUS; XENOPUS PROTEINS;

EID: 0035904233     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200106011     Document Type: Article

References (48)

1. Chromosome motion during attachment to the vertebrate spindle: Initial saltatory-like behavior of chromosomes and quantitative analysis of force production by nascent kinetochore fibers
2. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo
3. Phosphorylation by p34cdc2 protein kinase regulates binding of the kinesin-related motor HsEg5 to the dynactin subunit p150
4. The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro
5. NuMA is a component of an insoluble matrix at mitotic spindle poles
6. Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis
7. Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans
8. Ultraviolet microbeam irradiation of spindle fibres in crane-fly spermatocytes and newt epithelial cells: Resolution of previously conflicting observations
9. Motile properties of the kinesin-related Cin8p spindle motor extracted from Saccharomyces cerevisiae cells
10. Chromosome movement in mitosis requires microtubule anchorage at spindle poles
11. Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene
12. Spindle assembly in Xenopus egg extracts: Respective roles of centrosomes and microtubule self-organization
13. The kinesin-like protein KLP61F is essential for mitosis in Drosophila
14. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly
15. Preparation of modified tubulins
16. Force generation by microtubule assembly/disassembly in mitosis and related movements
17. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5
18. Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles
19. Cytoarchitecture and physical properties of cytoplasm: Volume, viscosity, diffusion, intracellular surface area
20. The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
21. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen
22. Mitosis and the physiology of cell division
23. Model for mitosis
24. Formation of spindle poles by dynein/dynactin-dependent transport of NuMA
25. Polewards microtubule flux in the mitotic spindle: Evidence from photoactivation of fluorescence
26. Dynamic instability of microtubule growth
27. The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle
28. The forces that move chromosomes in mitosis
29. Protein diffusion in living skeletal muscle fibers: Dependence on protein size, fiber type, and contraction
30. Rethinking mitosis
31. Chromosome motion and the spindle matrix
32. Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus)
33. Poleward microtubule flux mitotic spindles assembled in vitro
34. Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules
35. Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle
36. Mitotic spindle organization by a plus-end-directed microtubule motor
37. The disassembly and reassembly of functional centrosomes in vitro
38. Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus
39. The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles
40. Microtubule motors in mitosis
41. Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix
42. How microtubules get fluorescent speckles
43. Fluorescent speckle microscopy of microtubules: How low can you go?
44. Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells
45. Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities
46. Recombinant p50/dynamitin as a tool to examine the role of dynactin in intracellular processes
47. TPX2, a novel Xenopus MAP involved in spindle pole organization
48. The spindle: A dynamic assembly of microtubules and motors