Using real-time quantitative TaqMan RT-PCR to evaluate the role of dexamethasone in gene regulation of rat P-glycoproteins mdr1a/1b and cytochrome P450 3A1/2

Journal of Pharmaceutical Sciences

Volumn 93, Issue 10, 2004, Pages 2488-2496

  Mei, Qin ^a   Richards, Karen ^a   Strong Basalyga, Kristie ^a   Fauty, Scott E ^a   Taylor, Anne ^a   Yamazaki, Masayo ^a   Prueksaritanont, Thomayant ^a   Lin, Jiunn H ^a   Hochman, Jerome ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Cytochrome P450; Dexamethasone; Multidrug resistant genes; Regulation of P glycoprotein; TaqMan RT PCR

Indexed keywords

CYTOCHROME P450 3A1; CYTOCHROME P450 3A2; DEXAMETHASONE; GLYCOPROTEIN P; MESSENGER RNA; MONOCLONAL ANTIBODY; MULTIDRUG RESISTANCE PROTEIN 1; POLYCLONAL ANTIBODY;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN BLOOD VESSEL; COLON; CONTROLLED STUDY; GENE; GENE EXPRESSION REGULATION; KIDNEY; LIVER; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SMALL INTESTINE; TISSUE DISTRIBUTION; WESTERN BLOTTING;

EID: 4844226846     PISSN: 00223549     EISSN: None     Source Type: Journal    
DOI: 10.1002/jps.20102     Document Type: Article

References (38)

1. Borst P, Schinkel AH, Smit JJ, Wagenaar E, Van Deemter L, Smith AJ, Eijdems EW, Baas F, Zaman GJ. 1993. Classical and novel forms of multidrug resistance and the physiological functions of P-glycoproteins in mammals. Pharmacol Ther 60: 289-299.
2. Schinkel AH. 1997. The physiological function of drug-transporting P-glycoproteins. Semin Cancer Biol 8:161-170.
3. Ueda K, Clark DP, Chen CJ, Roninson IB, Gottesman MM, Pastan I. 1987. The human multidrug resistance (mdr1a) gene. J Biol Chem 262: 505-508.
4. Cordon-Cardo C, O'Brien JP, Boccia J, Casals D, Bertino JR, Melamed MR. 1990. Expression of the multidrug resistance gene product (P-glycoprotein) in human normal and tumor tissues. J Histochem Cytochem 38:1277-1287.
5. Fardel O, Lecureur V, Guillouzo A. 1996. The P-glycoprotein multidrug transporter. Gen Pharmacol 27:1283-1291.
6. Gan L-S L, Moseley MA, Khosla B, Augustijns PF, Bradshaw TP, Hendren RW, Thakker DR. 1996. CYP3A-Like cytochrome P450-mediated metabolism and polarized efflux of cyclosporin A in Caco-2 cells: Interaction between the two biochemical barriers to intestinal transport. Drug Metab Dispos 24:344-349.
7. Wacher VJ, Silverman JA, Zhang Y, Benet LZ. 1998. Role of P-glycoprotein and cytochrome P450 3A in limiting oral absorption of peptides and peptidomimetics. J Pharm Sci 87:1322-1330.
8. Watkins PB. 1997. The barrier function of CYP3A4 and P-glycoprotein in the small bowel. Adv Drug Del Rev 27:161-170.
9. Ito K, Kusuhara H, Sugiyama Y. 1999. Effects of intestinal CYP3A4 and P-glycoprotein on oral drug absorption - Theoretical approach. Pharm Res 16: 225-231.
10. Hochman JH, Chiba M, Nishime J, Yamazaki M, Lin JH. 2000. Influence of P-glycoprotein on the transport and metabolism of indinavir in Caco-2 cells expressing cytochrome P-450 3A4. J Pharmacol Exp Ther 292:310-318.
11. Hochman JH, Chiba M, Yamazaki M, Tang C, Lin JH. 2001. P-glycoprotein-mediated efflux of indinavir metabolites in Caco-2 cells expressing cytochrome P450 3A4. J Pharmacol Exp Ther 298: 323-330.
12. Hamman MA, Bruce MA, Haehner-Daniels BD, Hall SD. 2001. The effect of rifampin administration on the disposition of fexofenadine. Clin Pharmocol Ther 69:114-121.
13. Westphal K, Weinbrenner A, Zschiesche M, Franke G, Knoke M, Oertel R, Fritz P, von Richter O, Warzok R, Hachenberg T, Kauffman H-M, Schrenk D, Terhaag B, Kroemer HK, Siegmund W. 2000. Induction of P-glycoprotein by rifampin increases intestinal secretion of talinolol in human beings: A new type of drug/drug interaction. Clin Pharmocol Ther 68:345-355.
14. Schuetz EG, Beck WT, Schuetz JD. 1996. Modulators and substrates of P-glycoprotein and cytochrome P4503A coordinately up-regulate these proteins in human colon carcinoma cells. Mol Pharmacol 49:311-318.
15. Huang L, Wring SA, Woolley JL, Brouwer KR, Serabjit-Singh C, Polli JW. 2001. Induction of P-glycoprotein and cytochrome P450 3A by HIV protease inhibitors. Drug Metab Dispos 29:754-760.
16. Geick A, Eichelbaum M, Burk O. 2001. Nuclear receptor response elements mediate induction of intestinal MDR1 by rifampin. J Biol Chem 276: 14581-14587.
17. Greiner B, Eichelbaum M, Fritz P, Kreichgauer HP, von Richter O, Zundler J, Kroemer HK. 1999. The role of intestinal P-glycoprotein in the interaction of digoxin and rifampin. J Clin Invest 104: 147-153.
18. Schmittgen TD, Zakrajsek BA, Mills AG, Gorn V, Singer MJ, Reed MW. 2000. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: Comparison of endpoint and real-time methods. Anal Biochem 285:194-204.
19. Walker NA. 2002. Technique whose time has come. Science 269:557-559.
20. Sanchez JL, Storch GA. 2002. Multiplex, quantitative, real-time PCR assay for cytomegalovirus and human DNA. J Clin Microbiol 40:2381-2386.
21. Lecharduer D, Schwartz B, Scherman D. 1997. Rat brain microvessel endothelial cells: Primary cultures and immortalized differentiated cell lines. In: Drug transport across the blood-brain barrier: In vitro and in vivo techniques. Amsterdam: Harwood Academic Publishers, pp 27-36.
22. Audus KL, Borchardt RT. 1986. Characterization of an in vitro blood-brain barrier model system for studying drug transport and metabolism. Pharm Res 3:81-87.
23. Kim RB, Wandel C, Leake B, Cvetkovic M, Fromm MF, Dempsey PJ, Roden MM, Belas F, Chaudhary AK, Roden DM, Wood AJJ, Wilkinson GR. 1999. Interrelationship between substrates and inhibitors of human CYP 3A and P-glycoprotein. Pharm Res 16:408-414.
24. Synold TW, Dussault I, Forman BM. 2001. The orphan nucleair receptor SXR coordinately regulates drug metabolism and efflux. Nat Med 7:584-590.
25. Salphati L, Benet LZ. 1998. Modulation of P-glycoprotein expression by cytochrome P450 3A inducers in male and female rat livers. Biochem Pharmacol 55:387-395.
26. Yumoto R, Murakami T, Sanemasa M, Nasu R, Nagai J, Takano M. 2001. Pharmacokinetic interaction of cytochrome P450 3A-related compounds with rhodamine 123, a P-glycoprotein substrate, in rats pretreated with dexamethasone. Drug Metab Dispos 29:145-151.
27. Fardel O, Lecureur V, Guillouzo A. 1993. Regulation by dexamethasone of P-glycoprotein expression in cultured rat hematocytes. FEBS Lett 327: 189-193.
28. Schuetz JD, Silverman JA, Thottassery JV, Furuya KN, Schuetz EG. 1995. Divergent regulation of the class II P-glycoprotein gene in primary cultures of hepatocytes versus H35 hepatoma by glucocorticoids. Cell Growth Differ 6:1321-1332.
29. Zhao JY, Ikeguchi M, Eckersberg T, Kuo MT. 1993. Modulation of multidrug resistance gene expression by dexamethasone in cultured hepatoma cells. Endocrinology 133:521-528.
30. Pascussi JM, Drocourt L, Fabre JM, Maurel P, Vilarem MJ. 2000. Dexamethasone induces pregnane X receptor and retinoid X receptor-alpha expression in human hepatocytes: Synergistic increase of CYP3A4 induction by pregnane X receptor activators. Mol Pharmacol 58:361-372.
31. Pascussi JM, Drocourt L, Gerbal-Chaloin S, Fabre JM, Maurel P, Vilarem MJ. 2001. Dual effect of dexamethasone on CYP3A4 gene expression in human hepatocytes. Sequential role of glucocorticoid receptor and pregnane X receptor. Eur J Biochem 268:6346-6358.
32. Sukhai M, Yong A, Kalitsky J, Piquette-Miller M. 2000. Inflammation and interleukin 6 mediate reductions in the hepatic expression and transcription of the mdr1a and mdr1b genes. Mol Cell Biol Res Commun 4:248-256.
33. Kuo MT, Liu Z, Wei Y, Lin-Lee YC, Tatebe S, Mills GB, Unate H. 2002. Induction of human MDR1 gene expression by 2-acetylaminofluorene is mediated by effectors of the phosphoinositide 3-kinase pathway that activate NF-kappaB signaling. Oncogene 21:1945-1954.
34. Gill PK, Gescher A, Gant TW. 2001. Regulation of MDR1 promoter activity in human breast carcinoma cells by protein kinase C isozymes alpha and theta. Eur J Biochem 268:4151-4157.
35. Chin KV, Ueda K, Pastan I, Gottesman MM. 1992. Modulation of activity of the promoter of the human MDR1 gene by Ras and p53. Science 255: 459-462.
36. Moore LB, Parks DJ, Jones SA, Bledsoe RK, Consler TG, Stimmel JB, Goodwin B, Liddle C, Blanchard SG, Willson TM, Collins JL, Kliewer SA. 2000. Orphan nuclear receptors constitutive androstane receptor and pregnane X receptor share xenobiotic and steroid ligands. J Biol Chem 275: 15122-15127.
37. Labialle S, Gayet L, Marthinet R, Rigal D, Baggetto LG. 2002. Transcriptional regulators of the human multidrug resistance 1 gene: Recent views. Biochem Pharm 64:943-948.
38. Jin JY, Almon RR, Dubois DC, Jusko WJ. 2003. Modeling of the corticosteroid pharmacogenomics in rat liver using gene microarrays. J Pharmacol Exp Ther 307:93-109.