Cancer stem cells: Markers or biomarkers?

Cancer and Metastasis Reviews

Volumn 27, Issue 3, 2008, Pages 459-470

  Woodward, Wendy A ^a   Sulman, Erik P ^a  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Biomarkers; Breast cancer; Cancer stem cells; Colorectal cancer; Flow cytometry; Pancreatic cancer; Progenitors; Prostate cancer; Radiation therapy; Review

Indexed keywords

ACTIVATED LEUKOCYTE CELL ADHESION MOLECULE; ALPHA6 INTEGRIN; BETA1 INTEGRIN; BIOLOGICAL MARKER; CD133 ANTIGEN; CD24 ANTIGEN; CD31 ANTIGEN; CD45 ANTIGEN; ENDOTHELIAL PROTEIN C RECEPTOR; EPITHELIAL CELL ADHESION MOLECULE; HERMES ANTIGEN; NESTIN; PLATELET DERIVED GROWTH FACTOR ALPHA RECEPTOR; PROSTATE SPECIFIC ANTIGEN; TUMOR MARKER;

ANTIGEN EXPRESSION; BREAST CANCER; CANCER STEM CELL; CELL DIFFERENTIATION; CELL LINEAGE; CELL PROLIFERATION; CELL RENEWAL; COLORECTAL CANCER; FLOW CYTOMETRY; GENE EXPRESSION; GLIOBLASTOMA; GLIOMA; HEAD AND NECK CARCINOMA; HUMAN; MAMMARY GLAND; NONHUMAN; PANCREAS CANCER; PHENOTYPE; PRIORITY JOURNAL; PROSTATE CANCER; REVIEW; SOLID TUMOR; SQUAMOUS CELL CARCINOMA; TREATMENT RESPONSE;

ANIMALS; HUMANS; NEOPLASTIC STEM CELLS; TUMOR MARKERS, BIOLOGICAL;

EID: 47949086256     PISSN: 01677659     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10555-008-9130-2     Document Type: Review

References (106)

1. Bast Jr., R. C., Ravdin, P., Hayes, D. F., et al. (2001). 2000 update of recommendations for the use of tumor markers in breast and colorectal cancer: clinical practice guidelines of the American Society of Clinical Oncology. Journal of Clinical Oncology, 19(6), 1865-1878.
2. Hayes, D. F., Bast, R. C., Desch, C. E., et al. (1996). Tumor marker utility grading system: a framework to evaluate clinical utility of tumor markers. Journal of the National Cancer Institute, 88(20), 1456-1466.
3. Schilsky, R. L., & Taube, S. E. (2002). Tumor markers as clinical cancer tests-are we there yet? Seminars in Oncology, 29(3), 211-212.
4. Liao, M. J., Zhang, C. C., Zhou, B., et al. (2007). Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity. Cancer Research, 67(17), 8131-8138.
5. Kaplan, R. N., Psaila, B., & Lyden, D. (2007). Niche-to-niche migration of bone-marrow-derived cells. Trends in Molecular Medicine, 13(2), 72-81.
6. Psaila, B., Kaplan, R. N., Port, E. R., et al. (2006). Priming the 'soil' for breast cancer metastasis: the pre-metastatic niche. Breast Disease, 26, 65-74.
7. Yang, Z. J., & Wechsler-Reya, R. J. (2007). Hit 'em where they live: targeting the cancer stem cell niche. Cancer Cell, 11(1), 3-5.
8. Smith, G. H. (2006). Mammary stem cells come of age, prospectively. Trends in Molecular Medicine, 12(7), 287-289.
9. Weissman, I. L., Anderson, D. J., & Gage, F. (2001). Stem and progenitor cells: origins, phenotypes, lineage commitments, and transdifferentiations. Annual Review of Cell and Developmental Biology, 17, 387-403.
10. Shackleton, M., Vaillant, F., Simpson, K. J., et al. (2006). Generation of a functional mammary gland from a single stem cell. Nature, 439(7072), 84-88.
11. Stingl, J., Eirew, P., Ricketson, I., et al. (2006). Purification and unique properties of mammary epithelial stem cells. Nature, 439(7079), 993-997.
12. Al-Hajj, M., Wicha, M. S., Benito-Hernandez, A., et al. (2003). Prospective identification of tumorigenic breast cancer cells. Proceedings of the National Academy of Sciences of the United States of America, 100(7), 3983-3988.
13. Alvi, A. J., Clayton, H., Joshi, C., et al. (2003). Functional and molecular characterisation of mammary side population cells. Breast Cancer Research, 5(1), R1-8.
14. Goodell, M. A., Brose, K., Paradis, G., et al. (1996). Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. The Journal of Experimental Medicine, 183(4), 1797-1806.
15. Welm, B. E., Tepera, S. B., Venezia, T., et al. (2002). Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population. Developmental Biology, 245(1), 42-56.
16. Woodward, W. A., Chen, M. S., Behbod, F., et al. (2007). WNT/beta-catenin mediates radiation resistance of mouse mammary progenitor cells. Proceedings of the National Academy of Sciences of the United States of America, 104(2), 618-623.
17. Bao, S., Wu, Q., McLendon, R. E., et al. (2006). Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature, 444(7120), 756-760.
18. Phillips, T. M., McBride, W. H., & Pajonk, F. (2006). The response of CD24(-/low)/CD44+breast cancer-initiating cells to radiation. Journal of the National Cancer Institute, 98(24), 1777-1785.
19. Kordon, E. C., & Smith, G. H. (1998). An entire functional mammary gland may comprise the progeny from a single cell. Development (Cambridge, England), 125(10), 1921-1930.
20. Welm, B., Behbod, F., Goodell, M. A., et al. (2003). Isolation and characterization of functional mammary gland stem cells. Cell Proliferation, 36(Suppl 1), 17-32.
21. Sleeman, K. E., Kendrick, H., Ashworth, A., et al. (2006). CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells. Breast Cancer Research, 8(1), R7.
22. Asselin-Labat, M. L., Shackleton, M., Stingl, J., et al. (2006). Steroid hormone receptor status of mouse mammary stem cells. Journal of the National Cancer Institute, 98(14), 1011-1014.
23. Sleeman, K. E., Kendrick, H., Robertson, D., et al. (2007). Dissociation of estrogen receptor expression and in vivo stem cell activity in the mammary gland. The Journal of Cell Biology, 176(1), 19-26.
24. Dontu, G., Abdallah, W. M., Foley, J. M., et al. (2003). In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells. Genes & Development, 17(10), 1253-1270.
25. Proia, D. A., & Kuperwasser, C. (2006). Reconstruction of human mammary tissues in a mouse model. Nature Protocols, 1(1), 206-214.
26. Ponti, D., Costa, A., Zaffaroni, N., et al. (2005). Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer Research, 65(13), 5506-5511.
27. Woodward, W. A., Lucci, A., & Cristofanilli, M. (2007). A gene signature in breast cancer. The New England Journal of Medicine, 356(18), 1887-1888 author reply, 8).
28. Fillmore, C., & Kuperwasser, C. (2007). Human breast cancer stem cell markers CD44 and CD24: enriching for cells with functional properties in mice or in man? Breast Cancer Research, 9(3), 303.
29. Liu, R., Wang, X., Chen, G. Y., et al. (2007). The prognostic role of a gene signature from tumorigenic breast-cancer cells. The New England Journal of Medicine, 356(3), 217-226.
30. Abraham, B. K., Fritz, P., McClellan, M., et al. (2005). Prevalence of CD44+/CD24-/low cells in breast cancer may not be associated with clinical outcome but may favor distant metastasis. Clinical Cancer Research, 11(3), 1154-1159.
31. Shipitsin, M., Campbell, L. L., Argani, P., et al. (2007). Molecular definition of breast tumor heterogeneity. Cancer Cell, 11(3), 259-273.
32. Balic, M., Lin, H., Young, L., et al. (2006). Most early disseminated cancer cells detected in bone marrow of breast cancer patients have a putative breast cancer stem cell phenotype. Clinical Cancer Research, 12(19), 5615-5621.
33. Cristofanilli, M., Budd, G. T., Ellis, M. J., et al. (2004). Circulating tumor cells, disease progression, and survival in metastatic breast cancer. The New England Journal of Medicine, 351(8), 781-791.
34. Langer, I., Guller, U., Koechli, O. R., et al. (2007). Association of the presence of bone marrow micrometastases with the sentinel lymph node status in 410 early stage breast cancer patients: results of the Swiss Multicenter Study. Annals of Surgical Oncology, 14(6), 1896-903.
35. Slade, M. J., & Coombes, R. C. (2007). The clinical significance of disseminated tumor cells in breast cancer. Nature Clinical Practice, 4(1), 30-41.
36. Thurm, H., Ebel, S., Kentenich, C., et al. (2003). Rare expression of epithelial cell adhesion molecule on residual micrometastatic breast cancer cells after adjuvant chemotherapy. Clinical Cancer Research, 9(7), 2598-2604.
37. Camara, O., Rengsberger, M., Egbe, A., et al. (2007). The relevance of circulating epithelial tumor cells (CETC) for therapy monitoring during neoadjuvant (primary systemic) chemotherapy in breast cancer. Annals of Oncology, 18(9), 1484-1492.
38. Li, C., Heidt, D. G., Dalerba, P., et al. (2007). Identification of pancreatic cancer stem cells. Cancer Research, 67(3), 1030-1037.
39. Rodriguez, J. A., Li, M., Yao, Q., et al. (2005). Gene overexpression in pancreatic adenocarcinoma: diagnostic and therapeutic implications. World Journal of Surgery, 29(3), 297-305.
40. Wente, M. N., Jain, A., Kono, E., et al. (2005). Prostate stem cell antigen is a putative target for immunotherapy in pancreatic cancer. Pancreas, 31(2), 119-125.
41. Gu, Z., Yamashiro, J., Kono, E., et al. (2005). Anti-prostate stem cell antigen monoclonal antibody 1G8 induces cell death in vitro and inhibits tumor growth in vivo via a Fc-independent mechanism. Cancer Research, 65(20), 9495-500.
42. Cao, D., Ji, H., & Ronnett, B. M. (2005). Expression of mesothelin, fascin, and prostate stem cell antigen in primary ovarian mucinous tumors and their utility in differentiating primary ovarian mucinous tumors from metastatic pancreatic mucinous carcinomas in the ovary. International Journal of Gynecological Pathology, 24(1), 67-72.
43. Olempska, M., Eisenach, P. A., Ammerpohl, O., et al. (2007). Detection of tumor stem cell markers in pancreatic carcinoma cell lines. Hepatobiliary and Pancreatic Diseases International, 6(1), 92-97.
44. Esposito, I., Kleeff, J., Bischoff, S. C., et al. (2002). The stem cell factor-c-kit system and mast cells in human pancreatic cancer. Laboratory Investigation 82(11), 1481-1492.
45. Cunha, G. R., Hayward, S. W., Wang, Y. Z. (2002). Role of stroma in carcinogenesis of the prostate. Differentiation, 70(9-10), 473-485.
46. Isaacs, J. T., & Coffey, D. S. (1989). Etiology and disease process of benign prostatic hyperplasia. The Prostate, 2, 33-50.
47. Azuma, M., Hirao, A., Takubo, K., et al. (2005). A quantitative matrigel assay for assessing repopulating capacity of prostate stem cells. Biochemical and Biophysical Research Communications, 338(2), 1164-1170.
48. Hayward, S. W., Haughney, P. C., Rosen, M. A., et al. (1998). Interactions between adult human prostatic epithelium and rat urogenital sinus mesenchyme in a tissue recombination model. Differentiation, 63(3), 131-140.
49. Tsujimura, A., Koikawa, Y., Salm, S., et al. (2002). Proximal location of mouse prostate epithelial stem cells: a model of prostatic homeostasis. The Journal of Cell Biology, 157(7), 1257-1265.
50. Bhatia, B., Tang, S., Yang, P., et al. (2005). Cell-autonomous induction of functional tumor suppressor 15-lipoxygenase 2 (15-LOX2) contributes to replicative senescence of human prostate progenitor cells. Oncogene, 24(22), 3583-3595.
51. Hudson, D. L., O'Hare, M., Watt, F. M., et al. (2000). Proliferative heterogeneity in the human prostate: evidence for epithelial stem cells. Laboratory Investigation, 80(8), 1243-1250.
52. Burger, P. E., Xiong, X., Coetzee, S., et al. (2005). Sca-1 expression identifies stem cells in the proximal region of prostatic ducts with high capacity to reconstitute prostatic tissue. Proceedings of the National Academy of Sciences of the United States of America, 102(20), 7180-7185.
53. Xin, L., Lawson, D. A., & Witte, O. N. (2005). The Sca-1 cell surface marker enriches for a prostate-regenerating cell subpopulation that can initiate prostate tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America, 102(19), 6942-6947.
54. Reiter, R. E., Gu, Z., Watabe, T., et al. (1998). Prostate stem cell antigen: a cell surface marker overexpressed in prostate cancer. Proceedings of the National Academy of Sciences of the United States of America, 95(4), 1735-1740.
55. Amara, N., Palapattu, G. S., Schrage, M., et al. (2001). Prostate stem cell antigen is overexpressed in human transitional cell carcinoma. Cancer Research, 61(12), 4660-4665.
56. Han, K. R., Seligson, D. B., Liu, X., et al. (2004). Prostate stem cell antigen expression is associated with Gleason score, seminal vesicle invasion and capsular invasion in prostate cancer. The Journal of Urology, 171(3), 1117-1121.
57. Saffran, D. C., Raitano, A. B., Hubert, R. S., et al. (2001). Anti-PSCA mAbs inhibit tumor growth and metastasis formation and prolong the survival of mice bearing human prostate cancer xenografts. Proceedings of the National Academy of Sciences of the United States of America, 98(5), 2658-2663.
58. Patrawala, L., Calhoun, T., Schneider-Broussard, R., et al. (2006). Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells. Oncogene, 25(12), 1696-1708.
59. Collins, A. T., Berry, P. A., Hyde, C., et al. (2005). Prospective identification of tumorigenic prostate cancer stem cells. Cancer Research, 65(23), 10946-10951.
60. Richardson, G. D., Robson, C. N., Lang, S. H., et al. (2004). CD133, a novel marker for human prostatic epithelial stem cells. Journal of Cell Science, 117(Pt 16), 3539-3545.
61. Collins, A. T., Habib, F. K., Maitland, N. J., et al. (2001). Identification and isolation of human prostate epithelial stem cells based on alpha(2)beta(1)-integrin expression. Journal of Cell Science, 114(Pt 21), 3865-3872.
62. Liu, A. Y., True, L. D., LaTray, L., et al. (1997). Cell-cell interaction in prostate gene regulation and cytodifferentiation. Proceedings of the National Academy of Sciences of the United States of America, 94(20), 10705-10.
63. Prince, M. E., Sivanandan, R., Kaczorowski, A., et al. (2007). Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proceedings of the National Academy of Sciences of the United States of America, 104(3), 973-978.
64. Kawano, T., Yanoma, S., Nakamura, Y., et al. (2005). Soluble CD44 standard, CD44 variant 5 and CD44 variant 6 and their relation to staging in head and neck cancer. Acta Oto-laryngologica, 125(4), 392-397.
65. Kawano, T., Yanoma, S., Nakamura, Y., et al. (2005). Evaluation of soluble adhesion molecules CD44 (CD44st, CD44v5, CD44v6), ICAM-1, and VCAM-1 as tumor markers in head and neck cancer. American Journal of Otolaryngology, 26(5), 308-313.
66. Franzmann, E. J., Reategui, E. P., Pedroso, F., et al. (2007). Soluble CD44 is a potential marker for the early detection of head and neck cancer. Cancer Epidemiology, Biomarkers & Prevention, 16(7), 1348-1355.
67. Reategui, E. P., de Mayolo, A. A., Das, P. M., et al. (2006). Characterization of CD44v3-containing isoforms in head and neck cancer. Cancer Biology & Therapy, 5(9), 1163-1168.
68. Tijink, B. M., Buter, J., de Bree, R., et al. (2006). A phase I dose escalation study with anti-CD44v6 bivatuzumab mertansine in patients with incurable squamous cell carcinoma of the head and neck or esophagus. Clinical Cancer Research, 12(20 Pt 1), 6064-6072.
69. Wang, S. J., & Bourguignon, L. Y. (2006). Hyaluronan and the interaction between CD44 and epidermal growth factor receptor in oncogenic signaling and chemotherapy resistance in head and neck cancer. Archives of Otolaryngology, Head & Neck Surgery, 132(7), 771-778.
70. His, W. (1889). Die neuroblasten und deren enstehung im embryonalen mark. Abh Kgl Sachs Ges Wissensch Math Phys Kl, 15, 311-372.
71. Doe, C. Q., Fuerstenberg, S., & Peng, C. Y. (1998). Neural stem cells: from fly to vertebrates. Journal of Neurobiology, 36(2), 111-127.
72. Burd, G. D., & Nottebohm, F. (1985). Ultrastructural characterization of synaptic terminals formed on newly generated neurons in a song control nucleus of the adult canary forebrain. Journal of Comparative Neurology, 240(2), 143-152.
73. Goldman, S. A., & Nottebohm, F. (1983). Neuronal production, migration, and differentiation in a vocal control nucleus of the adult female canary brain. Proceedings of the National Academy of Sciences of the United States of America, 80(8), 2390-2394.
74. Cameron, H. A., Woolley, C. S., McEwen, B. S., et al. (1993). Differentiation of newly born neurons and glia in the dentate gyrus of the adult rat. Neuroscience, 56(2), 337-344.
75. Gage, F. H., Kempermann, G., Palmer, T. D., et al. (1998). Multipotent progenitor cells in the adult dentate gyrus. Journal of Neurobiology, 36(2), 249-266.
76. Kaplan, M. S., & Bell, D. H. (1984). Mitotic neuroblasts in the 9-day-old and 11-month-old rodent hippocampus. Journal of Neuroscience, 4(6), 1429-1441.
77. Gritti, A., Parati, E. A., Cova, L., et al. (1996). Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor. Journal of Neuroscience, 16(3), 1091-1100.
78. Sanai, H., Tramontin, A. D., Quinones-Hinojosa, A., et al. (2004). Unique astrocyte ribbon in adult human brain contains neural stem cells but lacks chain migration. Nature, 427(6976), 740-744.
79. Lois, C., Garcia-Verdugo, J. M., & Alvarez-Buylla, A. (1996). Chain migration of neuronal precursors. Science, 271(5251), 978-981.
80. Doetsch, F., Garcia-Verdugo, J. M., & Alvarez-Buylla, A. (1997). Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain. Journal of Neuroscience, 17(13), 5046-5061.
81. Sanai, N., Alvarez-Buylla, A., & Berger, M. S. (2005). Mechanisms of disease: Neural stem cells and the origin of gliomas. The New England Journal of Medicine, 353(8), 811-822.
82. Holland, E. C., Hively, W. P., Gallo, V., et al. (1998). Modeling mutations in the G1 arrest pathway in human gliomas: Overexpression of CDK4 but not loss of INK4a-ARF induces hyperploidy in cultured mouse astrocytes. Genes and Development, 12(23), 3644-3649.
83. Zhu, Y., Guignard, F., Zhao, D., et al. (2005). Early inactivation of p53 tumor suppressor gene cooperating with NF1 loss induces malignant astrocytoma. Cancer Cell, 8(2), 119-130.
84. Holland, E. C., Celestino, J., Dai, C., et al. (2000). Combined activation of Ras and Akt in neural progenitors induces glioblastoma formation in mice. Nature Genetics, 25(1), 55-57.
85. Reynolds, B. A., & Rietze, R. L. (2005). Neural stem cells and neurospheres-re-evaluating the relationship. Nature Methods, 2(5), 333-336.
86. Reynolds, B. A., & Weiss, S. (1992). Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science, 255(5052), 1707-1710.
87. Singh, S. K., Hawkins, C., Clarke, I. D., et al. (2004). Identification of human brain tumour initiating cells. Nature, 432(7015), 396-401.
88. Galli, R., Binda, E., Orfanelli, U., et al. (2004). Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma. Cancer Research, 64(19), 7011-7021.
89. Singh, S. K., Clarke, I. D., Terasaki, M., et al. (2003). Identification of a cancer stem cell in human brain tumors. Cancer Research, 63(18), 5821-5828.
90. Lee, J., Kotliarova, S., Kotliarov, Y., et al. (2006). Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell, 9(5), 391-403.
91. Hemmati, H. D., Nakano, I., Lazareff, J. A., et al. (2003). Cancerous stem cells can arise from pediatric brain tumors. Proceedings of the National Academy of Sciences of the United States of America, 100(25), 15178-15183.
92. Taylor, M. D., Poppleton, H., Fuller, C., et al. (2005). Radial glia cells are candidate stem cells of ependymoma. Cancer Cell, 8(4), 323-335.
93. Freije, W. A., Castro-Vargas, F. E., Fang, Z., et al. (2004). Gene expression profiling of gliomas strongly predicts survival. Cancer Research, 64(18), 6503-6510.
94. Haas-Kogan, D. A., Prados, M. D., Lamborn, K. R., et al. (2005). Biomarkers to predict response to epidermal growth factor receptor inhibitors. Cell Cycle 4(10), 1369-1372.
95. Nigro, J. M., Misra, A., Zhang, L., et al. (2005). Integrated array-comparative genomic hybridization and expression array profiles identify clinically relevant molecular subtypes of glioblastoma. Cancer Research, 65(5), 1678-1686.
96. Phillips, H. S., Kharbanda, S., Chen, R., et al. (2006). Molecular subclasses of high-grade glioma predict prognosis, delineate a pattern of disease progression, and resemble stages in neurogenesis. Cancer Cell, 9(3), 157-173.
97. Bao, S., Wu, Q., Sathornsumetee, S., et al. (2006). Stem cell-like glioma cells promote tumor angiogenesis through vascular endothelial growth factor. Cancer Research, 66(16), 7843-7848.
98. O'Brien, C. A., Pollett, A., Gallinger, S., et al. (2007). A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature, 445(7123), 106-110.
99. Ricci-Vitiani, L., Lombardi, D. G., Pilozzi, E., et al. (2007). Identification and expansion of human colon-cancer-initiating cells. Nature, 445(7123), 111-115.
100. Dalerba, P., Dylla, S. J., Park, I. K., et al. (2007). Phenotypic characterization of human colorectal cancer stem cells. Proceedings of the National Academy of Sciences of the United States of America, 104(24), 10158-10163.
101. Bar, E. E., Chaudhry, A., Lin, A., et al. (2007). Cyclopamine-mediated hedgehog pathway inhibition depletes stem-like cancer cells in glioblastoma. Stem Cells, 25, 2524-2533.
102. Chen, M. S., Woodward, W. A., Behbod, F., et al. (2007). Wnt/beta-catenin mediates radiation resistance of Sca1+ progenitors in an immortalized mammary gland cell line. Journal of Cell Science, 120(Pt 3), 468-477.
103. Bonner, J. A., Harari, P. M., Giralt, J., et al. (2006). Radiotherapy plus cetuximab for squamous-cell carcinoma of the head and neck. The New England Journal of Medicine, 354(6), 567-578.
104. Group, B. W. D. (2001). Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework. Clinical Pharmacology and Therapeutics, 69(3), 89-94.
105. Schmitt, M., Harbeck, N., Daidone, M. G., et al. (2004). Identification, validation, and clinical implementation of tumor-associated biomarkers to improve therapy concepts, survival, and quality of life of cancer patients: Tasks of the Receptor and Biomarker Group of the European Organization for Research and Treatment of Cancer. International Journal of Oncology, 25(5), 1397-1406.
106. Schmitt, M., Mengele, K., Schueren, E., et al. (2007). European Organisation for Research and Treatment of Cancer (EORTC) Pathobiology Group standard operating procedure for the preparation of human tumour tissue extracts suited for the quantitative analysis of tissue-associated biomarkers. European Journal of Cancer, 43(5), 835-844.