Chain migration of neuronal precursors

Science

Volumn 271, Issue 5251, 1996, Pages 978-981

  Lois, Carlos ^a   García Verdugo, Jose Manuel ^b   Alvarez Buylla, Arturo ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

GLIAL FIBRILLARY ACIDIC PROTEIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL MIGRATION; CELL TYPE; ELECTRON MICROSCOPY; MOUSE; NERVE CELL; NERVE CELL DIFFERENTIATION; NEUROBLAST; NONHUMAN; OLFACTORY BULB; PRIORITY JOURNAL; STEM CELL;

EID: 0030059989     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5251.978     Document Type: Article

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51. 4, dehydrated and embedded in LRwhite resin (London Resin Company, London), and cut into 60-nm sections Ultrathin sections were collected onto Forrnvar-coated nickel grids and processed for immunocytochemistry with antibodies to GFAP (Sigma, dilution 1:2500). Secondary antibodies (Amersham, dilution 1:20) were coupled to 10-nm colloidal gold particles. Sections were then stained with 2% uranyl acetate and examined with a Jeol 100CX electron microscope.
52. C. Lois, J.-M. García-Verdugo, A. Alvarez-Buylla, data not shown
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57. We thank F. Nottebohm, E. Font, and F. Doetsch for critically reading the manuscript, G. Rougon for antibodies to PSA-N-CAM, and E. Sphicas for help with postembedding immunocytochemistry. Supported by NIH grants NS28478 and HD32116 to A.A.B, and DGICYT PB91-0643 (Spain) to J.-M.G.-V. C L is a recipient of a La Caixa Foundation graduate program fellowship.