Selection and target-site mapping of peptides inhibiting HCV NS5B polymerase using phage display

Journal of Microbiology and Biotechnology

Volumn 18, Issue 2, 2008, Pages 328-333

  Kim, Min Soo ^a   Park, Chanhee ^a   Lee, Jong Ho ^a   Myung, Heejoon ^a  

^a Hankuk University of Foreign Studies   (South Korea)

Author keywords

Binding pocket; Hepatitis C virus; NS5B polymerase; Peptide inhibitor; Phage display

Indexed keywords

ALANINE; ANTIVIRUS AGENT; ASPARAGYLTYROSYLASPARAGYLLEUCYLSERYLARGINYLASPARAGYLLEUCYLTHREONYLTRYPTOPHANYLPHENYLALANYLTYROSINE; CYSTEINYLALANYLTHREONYLARGINYLLEUCYLGLYCYLARGINYLSERYLSERYLARGINYLCYSTEINE; CYSTEINYLMETHIONYLALANYLPHENYLALANYLPHENYLALANYLISOLEUCYLSERYLARGINYLTRYPTOPHANYLARGINYLCYSTEINE; GLUTATHIONE TRANSFERASE; NONSTRUCTURAL PROTEIN 5B; PEPTIDE DERIVATIVE; PROLINE; THREONYLGLYCYLPROLYLLEUCYLPROLYLLYSYLGLUTAMIC ACID; TRYPTOPHANYLSERYLARGINYLPROLYLARGINYLSERYLLEUCINE; PEPTIDE; PEPTIDE LIBRARY; RNA DIRECTED RNA POLYMERASE; VIRUS PROTEIN;

ANIMAL CELL; ANTIVIRAL ACTIVITY; ARTICLE; BINDING SITE; BIOPANNING; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DRUG STRUCTURE; DRUG SYNTHESIS; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PEPTIDE MAPPING; PHAGE DISPLAY; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; REPLICON; TWO HYBRID SYSTEM; VIRUS INHIBITION; VIRUS MUTANT; VIRUS REPLICATION; YEAST; AMINO ACID SEQUENCE; ANIMAL; CELL LINE; CHEMISTRY; DRUG EFFECT; ENZYMOLOGY; GENETICS; HAMSTER; METABOLISM; METHODOLOGY; PROTEIN BINDING; SYRIAN HAMSTER;

HEPATITIS C VIRUS;

AMINO ACID SEQUENCE; ANIMALS; ANTIVIRAL AGENTS; BINDING SITES; CELL LINE; CRICETINAE; HEPACIVIRUS; HUMANS; MESOCRICETUS; PEPTIDE LIBRARY; PEPTIDE MAPPING; PEPTIDES; PROTEIN BINDING; RNA REPLICASE; TWO-HYBRID SYSTEM TECHNIQUES; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 46449119850     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. Adachi, T., H. Ago, N. Habuka, K. Okuda, M. Komatsu, S. Ikeda, and K. Yatsunami. 2002. The essential role of C-terminal residues in regulating the activity of hepatitis C virus RNA-dependent RNA polymerase. Biochim. Biophys. Acta 1601: 38-48.
2. Adda, C. G., R. F. Anders, L. Tilley, and M. Foley, 2002. Random sequence libraries displayed on phage: Identification of biologically important molecules. Comb. Chem. High Throughput Screen. 5: 1-14.
3. Amin, A., J. Zaccardi, S. Mullen, S. Olland, M. Orlowski, B. Fold, P. Labonte. and P. Mak. 2003. Identification of constrained peptides that bind to and preferentially inhibit the activity of the hepatitis C viral RNA-dependent RNA polymerase. Virology 15: 158-169.
4. Beaulieu, P. L. and Y. S. Tsantrizos. 2004. Inhibitors of the HCV NS5B polymerase: New hope for the treatment of hepatitis C infections. Curr. Opin. Investig. Drugs 5: 838-850.
5. Bressanelli, S., L. Tomei, A. Roussel, I. Incitti, R. L. Vitale, M. Mathieu, R. De Francesco, and F. A. Rey. 1999. Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus. Proc. Natl. Acad. Sci. USA 96: 13034-13039.
6. Bressanelli, S., L. Tomei, F. A. Rey, and R. De Francesco. 2002. Structural analysis of the hepatitis C virus RNA polymerase in complex with ribonucleotides. J. Virol. 76: 3482-3492.
7. Chan, L., O. Pereira, T. J. Reddy, S. K. Das, C. Poisson, M. Courchesne, M. Proulx, A. Siddiqui, C. G. Yannopoulos, et al. 2004. Discovery of thiophene-2-carboxylic acids as potent inhibitors of HCV NS5B polymerase and HCV subgenomic RNA replication. Part 2: Tertiary amides. Bioorg. Med. Chem. Lett. 14: 797-800.
8. Cohen, J. 1999. The scientific challenge of hepatitis C. Science 258: 26-30.
9. De Francesco, R. and G. Migliaccio. 2005. Challenges and successes in developing new therapies for hepatitis C. Nature 436: 953-960.
10. Gowans, L. J., K. L. Jones, M. Bharadwaj, and C. C. Jackson. 2004. Prospects for dendritic cell vaccination in persistent infection with hepatitis C virus. J. Clin. Virol. 30: 283-290.
11. Hantusch. B., S. Krieger, E. Untersmayr, I. Scho, R. Knittelfelder, S. Flicker, S. Spitzauer, R. Valenta. G. Boltz-Nitulescu, et al. 2004. Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library. J. Allergy Clin. Immunol. 114: 1294-1300.
12. Hwang, B. and S.-W. Lee. 2005. Analysis of in vivo interaction of HCV NS3 protein and specific RNA aptamer with yeast three-hybrid system. J. Microbiol. Biotechnol. 15: 660-664.
13. Ichii, K., Y. Tanaka, C.-C. Yap, H. Aizaki, Y. Matsumura, and T. Miyamura. 1999. Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding. Hepatology 29: 1227-1235.
14. Kim, J., H. Park, K. Choi, J. Lee, J. Park, and M. Yoon. 2006. Screening of peptides bound to anthrax protective antigen by phage display. J. Microbiol. Biotechnol. 16: 1784-1790.
15. Kofler, M., K. Motzny, M. Beyermann, and C. Freund. 2005. Novel interaction partners of the CD2BP2-GYF domain. J. Biol. Chem. 280: 33397-33402.
16. Krieger, N., V. Lohmann, and R. Bartenschlager. 2001. Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations. J. Virol. 75: 4614-4624.
17. Lecut, C., V. Arocas, H. Ulrichts, A. Elbaz, J. Villeval, J. Lacapene, H. Deckmyn, and M. Jandrot-Perrus. 2004. Identification of residues within human glycoprotein VI involved in the binding to collagen. J. Biol. Chem. 279: 52293-52299.
18. Lee, J.-H., I. Y. Nam, and H. Myung. 2006. Nonstructural protein 5B of hepatitis C virus. Mol. Cells 21: 330-336.
19. Leveque, V. J.-P. and Q. M. Wang. 2002. RNA-dependent RNA polymerase encoded by hepatitis C virus: Biomedical applications. Cell. Mol. Life Sci. 59: 909-919.
20. Lohmann, V., A. Roos, F. Komer, J.-O. Koch, and R. Bartenschlager. 2000. Biochemical and structural analysis of the NS5B RNA-dependent RNA polymerase of the hepatitis C virus. J. Viral Hepat. 7: 167-174.
21. McHutchison, J. G. and T. Poynard. 1999. Combination therapy with interferon plus ribavirin for the initial treatment of chronic hepatitis C. Semin. Liver Dis. 19: 57-65.
22. Moradpour, D. and H. E. Blum. 2004. A primer on the molecular virology of hepatitis C. Liver Int. 24: 519-525.
23. Nguyen, T. T., A. T. Gates, L. L. Gutshall, V. K. Johnston, B. Gu, K. J. Duffy, and R. T. Sarisky. 2003. Resistance profile of a hepatitis C virus RNA-dependent RNA polymerase benzothiadiazine inhibitor. Antimicrob. Agents Chemother. 47: 3525-3530.
24. Pogam, S. L., H. Kang, S. F. Harris, V. Leveque, A. M. Giannetti, S. Ali, W.-R. Jiang, S. Rajyaguru, G, Tavares, et al. 2006. Selection and characterization of replicon variants dually resistant to thumb- and palm-binding nonnucleoside polymerase inhibitors of the hepatitis C virus. J. Virol. 80: 6146-6154.
25. Purcell, R. 1994. Hepatitis viruses: Changing patterns of human disease. Proc. Natl. Acad. Sci. USA 91: 2401-2406.
26. Rasmusen, U. B., V. Schreiber, H. Schultz, F. Mischler, and K. Schughart. 2002. Tumor cell-targeting by phage-displayed peptides. Cancer Gene Ther. 9: 606-612.
27. Rowley, M. J., M. Scealy, J. C. Whisstock, J. A. Jois, L. C. Wijeyewickrema, and I. R. Mackay. 2000. Prediction of the immunodominant epitope of the pyruvate dehydrogenase complex E12 in primary biliary cirrhosis using phage display. J. Immunol. 164: 3413-3419.
28. 2 nanoparticles. J. Microbiol. Biotechnol. 16: 303-307.
29. Shin. K., J. Lim, J. Kim, H. Myung, and S.-W. Lee. 2006. Inhibition of replication of hepatitis C virus replicon with nuclease-resistant RNA aptamers. J. Microbiol. Biotechnol. 16: 1634-1639.
30. Sidhu, S., W. Fairbrother, and K. Deshayes. 2003. Exploring protein-protein interactions with phage display. ChemBioChen 4: 14-25.
31. Smith, G. P. 1985. Filamentous Fusion phage: Novel expression vectors that display cloned antigens on the vision surface. Science 228: 1315-1317.
32. Suzuki, R., T. Suzuki, K. Ishii, Y. Matsuura, and T. Miyamura. 1999. Processing and functions of hepatitis C virus proteins. Intervirology 42: 145-152.
33. Tomei, L., S. Altamura, L. Bartholomew, A. Biroccio, A. Ceccacci, L. Pacini, F. Naijes, N. Gennari, M. Bisbocci, et al. 2003. Mechanism of action and antiviral activity of benzimidazole-based allosteric inhibitors of the hepatitis C virus RNA-dependent RNA polymerase. J. Virol. 77: 13225-13231.
34. Tomei, L., S. Altamura, L. Bartholomew, M. Bisbocci, C. Bailey, M. Bosserman, A. Celluccι, E. Forte, I. Incιttι, et al. 2004. Characterization of the inhibition of hepatitis C virus RNA replication by nonnucleosides. J. Virol. 78: 938-946.
35. Wang, C., P. Sarnow, and A. Siddiqui. 1993. Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism. J. Virol. 67: 3338-3344.
36. Wang, M., K. K. Ng, M. M. Cherney, L. Chan, C. Q Yannopoulos, J. Bedard, N. Morin, N. Nguyen-Ba, M. H. Alaoui-ismaili, et al. 2003. Non-nucleoside analogue inhibitors bind to an allosteric site on HCv NS5B polymerase. Crystal structures and mechanism of inhibition. J. Biol. Chem. 278: 9489-9495.
37. Wasley, A. and M. J. Alter. 2000, Epidemiology of hepatitis C: Geographic differences and temporal trends. Semin. Liver Dis. 20: 1-16.
38. Yang, Z., W. Shm, M. Kim, K. Lee, K. Kim, K. Kim, C. Kim, S. Ha, and D. Chung. 2007. Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine. J. Microbiol. Biotechnol. 17: 571-578.