Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations

Journal of Virology

Volumn 75, Issue 10, 2001, Pages 4614-4624

  Krieger, N ^a   Lohmann, V ^a   Bartenschlager, R ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

KANAMYCIN KINASE; LUCIFERASE; VIRUS RNA;

AMINO ACID SUBSTITUTION; ARTICLE; CELL CULTURE; COLONY FORMATION; CONTROLLED STUDY; GENE REPLICATION; GENE SEQUENCE; GENETIC CODE; GENETIC TRANSFECTION; GENOME; HEPATITIS C VIRUS; MARKER GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REPLICON; SEQUENCE ANALYSIS; VIRAL GENETICS; VIRUS MUTATION; VIRUS REPLICATION;

ADAPTATION, PHYSIOLOGICAL; CELL CULTURE TECHNIQUES; GENES, REPORTER; HEPACIVIRUS; HUMANS; LUCIFERASES; MUTATION; REPLICON; RNA, VIRAL; TUMOR CELLS, CULTURED; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 0035032237     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.10.4614-4624.2001     Document Type: Article

References (48)

1. Noncytopathic Sindbis virus RNA vectors for heterologous gene expression
2. Replication of hepatitis C virus
3. Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions
4. Kinetic and structural analyses of hepatitis C virus polyprotein processing
5. Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus
6. Efficient initiation of HCV RNA replication in cell culture
7. Effective amplification of long targets from cloned inserts and human genomic DNA
8. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
9. Complete nucleotide sequence of an attenuated hepatitis A virus: Comparison with wild-type virus
10. Mutations within the 5′ nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells
11. 2B and 2C mutations are essential but mutations throughout the genome of HAV contribute to adaptation to cell culture
12. Comparison of full-length sequences of interferon-sensitive and resistant hepatitis C virus 1b. Sensitivity to interferon is conferred by amino acid substitutions in the NS5A region
13. Mutations in the nonstructural protein 5A gene and response to interferon in patients with chronic hepatitis C virus lb infection
14. Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins
15. Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells
16. Evidence that hepatitis C virus resistance to interferon is mediated through repression of the PKR protein kinase by the nonstructural 5A protein
17. Control of PKR protein kinase by hepatitis C virus nonstructural 5A protein: Molecular mechanism of kinase regulation
18. Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants
19. A second hepatitis C virus-encoded proteinase
20. Characterization of the hepatitis C virus-encoded serine proteinase: Determination of proteinase-dependent polyprotein cleavage sites
21. Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus
22. Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis
23. Hepatitis C virus NS5A protein is phosphorylated in vitro by a stably bound protein kinase from HeLa cells and by cAMP-dependent protein kinase A-alpha catalytic subunit
24. Complete nucleotide sequence of a cell culture-adapted variant of hepatitis A virus: Comparison with wild-type virus with restricted capacity for in vitro replication
25. Production of two phsosphoproteins from the NS5A region of the hepatitis C viral genome
26. C-terminal domain of the hepatitis C virus NS3 protein contains an RNA helicase activity
27. Processing in the hepatitis C virus E2-NS2 region: Identification of p7 and two distinct E2-specific products with different C termini
28. Hepatitis C virus NS3 serine proteinase: Trans-cleavage requirements and processing kinetics
29. Mutations in hepatitis C virus RNAs conferring cell culture adaptation
30. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line
31. Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity
32. Two hepatitis C virus glycoprotein E2 products with different C termini
33. Growth of human hepatoma cells lines with differentiated functions in chemically defined medium
34. A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initiation of hepatitis C and classical swine fever virus RNAs
35. Characterization of cell lines carrying self-replicating hepatitis C virus RNAs
36. Natural history of HCV infection
37. Identification of the major phosphorylation site of the hepatitis C virus H strain NS5A protein as serine 2321
38. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties
39. Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo: Properties of the NS5A-associated kinase
40. Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes
41. Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing
42. Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A
43. NS3 is a serine protease required for processing of hepatitis C virus polyprorein
44. Internal ribosome entry site within hepatitis C virus RNA
45. Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form
46. Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism
47. RNA-dependent RNA polymerase activity of the soluble recombinant hepatitis C virus NS5B protein truncated at the C-terminal region