Conformational dynamics of free and catalytically active thermolysin are indistinguishable by hydrogen/deuterium exchange mass spectrometry

Biochemistry

Volumn 47, Issue 24, 2008, Pages 6342-6351

  Liu, Yu Hong ^a   Konermann, Lars ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDES; AMINES; BIOCHEMICAL ENGINEERING; ELECTROSPRAY IONIZATION; ENZYMES; FOOD ADDITIVES; FUNCTIONS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; MASS SPECTROMETRY; MECHANICS; PROTEINS; SPECTROMETERS; SPECTROMETRY; SPECTRUM ANALYSIS; X RAY OPTICS; ZINC; ZINC SULFIDE;

ACTIVE SITE RESIDUES; ACTIVE SITES; AMERICAN CHEMICAL SOCIETY (ACS); BALANCE (WEIGHTING); CATALYTIC ACTIVITIES; CATALYTIC CYCLING; CONFORMATIONAL DYNAMICS; DETERMINISTIC MECHANISM; ELECTROSPRAY IONIZATION MASS SPECTROMETRY (ESI_MS); ENZYME FUNCTIONING; FLEXIBLE ELEMENTS; FREE ENZYMES; HYDROGEN-DEUTERIUM EXCHANGE (HDX); INTERNAL MOBILITY; PEPTIDE MAPPING; PERIPHERAL (SPI); PRODUCT RELEASE; REFOLDING; RIGID ENVIRONMENT; STEADY-STATE CATALYSIS; STOCHASTIC ELEMENTS; STRUCTURAL CHANGES; STRUCTURAL FLUCTUATIONS; X RAY DATA;

DYNAMICS;

DEUTERIUM; HYDROGEN; PROTEINASE; THERMOLYSIN;

ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STRUCTURE; STEADY STATE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; BACILLUS; BACTERIAL PROTEINS; CATALYSIS; DEUTERIUM EXCHANGE MEASUREMENT; ENKEPHALIN, LEUCINE; ENZYME ACTIVATION; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY; THERMODYNAMICS; THERMOLYSIN;

EID: 45749110950     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi800463q     Document Type: Article

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