Identification of a novel human Rad51 variant that promotes DNA strand exchange

Nucleic Acids Research

Volumn 36, Issue 10, 2008, Pages 3226-3234

  Park, Jung Young ^a,b   Yoo, Han Wook ^c   Kim, Bok Ryang ^a   Park, Raekil ^a   Choi, Sang Yun ^b   Kim, Youngho ^a  

^a Wonkwang University   (South Korea)

^b Korea University   (South Korea)

^c Asan Medical Center   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; RAD51 PROTEIN; SINGLE STRANDED DNA; RAD51 PROTEIN, HUMAN;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CODON; CONTROLLED STUDY; DNA RECOMBINATION; DNA REPAIR; DNA STRAND BREAKAGE; EXON; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; TESTIS; TISSUE SPECIFICITY; WESTERN BLOTTING; CHEMISTRY; ENZYMOLOGY; GENETICS; MALE; METABOLISM; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; RNA SPLICING; SEQUENCE HOMOLOGY; TISSUE DISTRIBUTION;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CELL NUCLEUS; HUMANS; MALE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RAD51 RECOMBINASE; RNA SPLICING; SEQUENCE HOMOLOGY, AMINO ACID; TESTIS; TISSUE DISTRIBUTION;

EID: 45549084115     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkn171     Document Type: Article

References (51)

1. Haber,J.E. (1999) DNA recombination: The replication connection. Trends Biochem. Sci., 24, 271-275.
2. West,S.C. (2003) Molecular views of recombination proteins and their control. Nat. Rev. Mol. Cell Biol., 4, 435-445.
3. Bertrand,P., Saintigny,Y. and Lopez,B.S. (2004) p53's double life: transactivation-independent repression of homologous recombination. Trends Genet., 20, 235-243.
4. Baumann,P. and West,S.C. (1998) Role of the human RAD51 protein in homologous recombination and doublestranded-break repair. Trends Biochem. Sci., 23, 247-251.
5. Game,J.C. (1993) DNA double-strand breaks and the RAD50-RAD57 genes in Saccharomyces. Semin. Cancer Biol., 4, 73-83.
6. Shinohara,A., Ogawa,H., Matsuda,Y., Ushio,N., Ikeo,K. and Ogawa,T. (1993) Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat. Genet., 4, 239-243.
7. Sturzbecher,H.W., Donzelmann,B., Henning,W., Knippschild,U. and Buchhop,S. (1996) p53 is linked directly to homologous recombination processes via RAD51/RecA protein interaction. EMBO J., 15, 1992-2002.
8. Buchhop,S., Gibson,M.K., Wang,X.W., Wagner,P., Sturzbecher,H.W. and Harris,C.C. (1997) Interaction of p53 with the human Rad51 protein. Nucleic Acids Res., 25, 3868-3874.
9. Sharan,S.K., Morimatsu,M., Albrecht,U., Lim,D.S., Regel,E., Dinh,C., Sands,A., Eichele,G., Hasty,P. and Bradley,A. (1997) Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2. Nature, 386, 804-810.
10. Scully,R., Chen,J., Ochs,R.L., Keegan,K., Hoekstra,M., Feunteun,J. and Livingston,D.M. (1997) Dynamic changes of BRCA1 subnuclear location and phosphorylation state are initiated by DNA damage. Cell, 90, 425-435.
11. Yuan,S.S., Lee,S.Y., Chen,G., Song,M., Tomlinson,G.E. and Lee,E.Y. (1999) BRCA2 is required for ionizing radiation-induced assembly of Rad51 complex in vivo. Cancer Res., 59, 3547-3551.
12. Han,H., Bearss,D.J., Browne,L.W., Calaluce,R., Nagle,R.B. and Von Hoff,D.D. (2002) Identification of differentially expressed genes in pancreatic cancer cells using cDNA microarray. Cancer Res., 62 2890-2896.
13. Raderschall,E., Stout,K., Freier,S., Suckow,V., Schweiger,S. and Haaf,T.E. (2002) Elevated levels of Rad51 recombination protein in tumor cells. Cancer Res., 62, 219-225.
14. Xia,S.J., Shammas,M.A. and Shmookler Reis,R.J. (1997) Elevated recombination in immortal human cells is mediated by HsRAD51 recombinase. Mol. Cell. Biol., 17, 7151-7158.
15. Albala,J.S., Thelen,M.P., Prange,C., Fan,W., Christensen,M., Thompson,L.H. and Lennon,G.G. (1997) Identification of a novel human RAD51 homolog, RAD51B. Genomics, 46, 476-479.
16. Dosanjh,M.K., Collins,D.W., Fan,W., Lennon,G.G., Albala,J.S., Shen,Z. and, Schild,D. (1998) Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes. Nucleic Acids Res. 26, 1179-1184.
17. Liu,N., Lamerdin,J.E., Tebbs,R.S., Schild,D., Tucker,J.D., Shen,M.R., Brookman,K.W., Siciliano,M.J., Walter,C.A., Fan,W. et al. (1998) XRCC2 and XRCC3, new hurtian Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages. Mol. Cell, 1, 783-793.
18. Pittman,D.L., Weinberg,L.R. and Schimenti,J.C. (1998) identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene. Genomics, 49, 103-111.
19. Lin,Z., Kong,H., Nei,M. and Ma,H. (2006) Origins and evolution of the recA/RAD51 gene family: Evidence for ancient gene duplication and endosymbiotic gene transfer. Proc. Natl Acad. Sci. USA, 103, 10328-10333.
20. Miller, K.A., Sawicka,D., Barsky,D. and Albala,J.S. (2004) Domain mapping of the Rad51 paralog protein complexes. Nucleic Acids Res. 32, 169-178.
21. Griffin,C.S., Simpson,P.J., Wilson,C.R. and Thacker,J. (2000) Mammalian recombination-repair genes XRCC2 and XRCC3 promote correct chromosome segregation. Nat. Cell Biol., 2, 757-761.
22. Takata,M., Sasaki,M.S., Tachiiri,S., Fukushima,T., Sonoda,E., Schild,D., Thompson,L.H. and Takeda,S. (2001) Chromosome instability and defective recombinational repair in knockout mutants of the five Rad51 paralogs. Mol. Cell. Biol., 21, 2858-2566.
23. Drexler,G.A., Rogge,S., Beisker,W., Wckardt-Schupp,F., Zdzienicka,M.Z. and Fritz,E. (2004) Spontaneous homologous recombination is decreased in Rad51C-deficient hamster cells. DNA Repair, 3, 1335-1343.
24. Kurumizaka,H., Aihara,H., Kagawa,W., Shibata,T. and Yokoyama,S. (1999) Human Rad51 amino acid residues required for Rad52 binding. J. Mol. Biol., 291, 537-548.
25. Kim,M.S., Kim,S.S., Jung,S.T., Park,J.Y., Yoo,H.W., Ko,J., Csiszar,K., Choi,S.Y., and Kim,Y. (2003) Expression and purification of enzymatically active forms of the human lysyl oxidase-like protein 4. J. Biol. Chem., 278, 52071-52074.
26. Sung,P. and Robberson,D.L. (1995) DNA strand exchange mediated by a Rad51-ssDNA nucleoprotein filament with polarity opposite to that of RecA. Cell, 82, 453-461.
27. Shinohara,A. and Ogawa,T. (1998) Stimulation by Rad52 of yeast Rad51 mediated recombination. Nature, 391, 404-407.
28. Li,S. and Wilkinson,M.F. (1997) Site-directed mutagenesis: A two-step method using PCR and DpnI. Biotechniques, 23, 588-590.
29. Benedict,R.C. and Kowalczykowski,S.C. (1988) Increase of the DNA strand assimilation activity of recA protein by removal of the C terminus and structure-function studies of the resulting protein fragment. J. Biol. Chem., 263, 15513-15520.
30. Tateishi,S., Horii,T., Ogawa,T. and Ogawa,H., (1992) C-terminal truncated Escherichia coli RecA protein RecA5327 has enhanced binding affinities to single- and double-stranded DNAs. J. Mol. Biol. 223, 115-129.
31. Lusetti,S.L., Wood,E.A., Fleming,C.D., Modica,M.J., Korth,J., Abbott,L., Dwyer,D.W., Roca,A.I., Inman,R.B. and Cox,M.M. (2003) C-terminal deletions of the Escherichia coli RecA protein. Characterization of in vivo and in vitro effects. J. Biol. Chem., 278, 16372-16380.
32. Subramaniam,P.S., Mujtaba,M.G., Paddy,M.R. and Johnson,H.M. (1999) The carboxyl terminus of interferon-gamma contains a functional polybasic nuclear localization sequence. J. Biol. Chem., 274, 403-407.
33. Xiao,Z., Latek,R. and Lodish,H.F. (2003) An extended bipartite nuclear localization signal in Smad4 is required for its nuclear import and transcriptional activity. Oncogene, 20, 1057-1069.
34. Habu,T., Taki,T., West,A., Nishimune,Y. and Morita,T. (1996) The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis. Nucleic Acids Res., 24, 470-477.
35. Kito,K., Wada,H., Yeh,E.T. and Kamitani,T. (1999) Identification of novel isoforms of human RAD52. Biochim. Biophys. Acta, 1489, 303-314.
36. Kawabata,M. and Saeki,K. (1999) Multiple alternative transcripts of the human homologue of the mouse TRAD/R51H3/RAD51D gene, a member of the rec A/RAD51 gene family. Biochem. Biophys. Res. Commun., 257, 156-162.
37. Kawabata,M., Akiyama,K. and Kawabata,T. (2004) Genomic structure and multiple alternative transcripts of the mouse TRAD/RAD51L3/RAD51D gene, a member of the recA/RAD51 gene family. Biochim. Biophys. Acta, 1679, 107-116.
38. Thorpe,P.H., Marrero,V.A., Savitzky,M.H., Sunjevaric,I., Freeman,T.C. and Rothstein,R. (2006) Cells expressing murine RAD52 splice variants favor sister chromatid repair. Mol. Cell. Biol., 26, 3752-3763.
39. New,J.H., Sugiyama,T., Zaitseva,E. and Kowalczykowski,S.C. (1998) Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature, 391, 407-410.
40. Kumar,J.K. and Gupta,R.C. (2004) Strand exchange activity of human recombination protein Rad52. Proc. Natl Acad. Sci. USA, 101, 9562-9567.
41. Tarsounas,M., Davies,D. and West,S.C. (2003) BRCA2-dependent and independent formation of RAD51 nuclear foci. Oncogene, 27, 1115-1123.
42. French,K.A., Tambini,C.E. and Thacker,J. (2003) Identification of functional domains in the RAD51L2 (RAD51C) protein and its requirement for gene conversion. J. Biol. Chem., 278, 45445-45450.
43. Miller,K.A., Hinz,J.M., Yamada,N.A., Thompson,L.H. and Albala,J.S. (2005) Nuclear localization of Rad51B is independent of Rad51C and BRCA2. Mutagenesis, 20, 57-63.
44. Saintigny,Y., Rouillard,D., Chaput,B., Soussi,T. and Lopez,B.S. (1999) Mutant p53 proteins stimulate spontaneous and radiation-induced intrachromosomal homologous recombination independently of the alteration of the transactivation activity and of the G1 checkpoint. Oncogene, 18, 3553-3563.
45. Linke,S.P., Sengupta,S., Khabie,N., Jeffries,B.A., Buchhop,S., Miska,S., Henning,W., Pedeux,R., Wang,X.W., Hofseth,L.J. et al. (2003) p53 interacts with hRAD51 and hRAD54, and directly modulates homologous recombination. Cancer Res., 63, 2596-2605.
46. Benson,F.E., Stasiak,A. and West,S.C. (1994) Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA. EMBO J., 13, 5764-5771.
47. Wong,A.K., Pero,R., Ormonde,P.A., Tavtigian,S.V. and Bartel,P.L. (1997) RAD51 interacts with the evolutionarily conserved BRC motifs in the human breast cancer susceptibility gene brca2. J. Biol. Chem., 272, 31941-31944.
48. Chen,J., Silver,D.P., Walpita,D., Cantor,S.B., Gazdar,A.F., Tomlinson,G., Couch,F.J., Weber,B.L., Ashley,T., Livingston,D.M. et al. (1998) Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells. Mol. Cell 2, 317-328.
49. Wooster,R., Bignell,G., Lancaster,J., Swift,S., Seal,S., Mangion,J., Collins,N., Gregory,S., Gumbs,C., Micklem,G. et al. (1995) Identification of the breast cancer susceptibility gene BRCA2. Nature 378, 789-792.
50. Tavtigian,S.V., Simard,J., Rommens,J., Couch,F., Shattuckeidens,D., Neuhausen,S., Merajver,S., Thorlacius,S., Offit,K., Stoppalyonnet,D. et al. (1996) The complete BRCA2 gene and mutations in chromosome 13q-linked kindreds. Nat. Genet., 12, 333-337.
51. Galkin,V.E., Esashi,F., Yu,X., Yang,S., West,S.C. and Egelman,E.H. (2005) BRCA2 BRC motifs bind RAD51-DNA filaments. Proc. Natl Acad. Sci. USA., 102, 8537-8542.