Site-directed mutagene sis: A two-step method using PCR and DpnI

BioTechniques

Volumn 23, Issue 4, 1997, Pages 588-590

  Li, Shulin ^a   Wilkinson, Miles F ^a  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

T LYMPHOCYTE RECEPTOR BETA CHAIN; ENDODEOXYRIBONUCLEASE DPNI; LYMPHOCYTE ANTIGEN RECEPTOR; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; EXON; EXPRESSION VECTOR; GENE TECHNOLOGY; MOLECULAR CLONING; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; ANIMAL; GENETICS; METABOLISM; METHODOLOGY;

ANIMALS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; MICE; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA;

EID: 0030608942     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97234bm05     Document Type: Article

References (3)

1. Carter, M.S., J. Doskow, P. Morris, S. Li, R.P. Nhim, S. Sandstedt and M.F. Wilkinson. 1995. A regulatory mechanism that detects premature nonsense codons in T-cell receptor transcripts in vivo is reversed by protein synthesis inhibitors in vitro. J. Biol. Chem. 270:28995-29003.
2. Chen, B. and A.E. Przybyla. 1994. An efficient site-directed mutagenesis method based on PCR. BioTechniques 17:657-659.
3. Good, L. and R.N. Nazar. 1992. An improved thermal cycle for two-step PCR-based targeted mutagenesis. Nucleic Acids Res. 20:4934.