The RNA-induced silencing complex is a Mg2+-dependent endonuclease

Current Biology

Volumn 14, Issue 9, 2004, Pages 787-791

  Schwarz, Dianne S ^a   Tomari, Yukihide ^a   Zamore, Phillip D ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; DROSOPHILA PROTEIN; ENDONUCLEASE; LUCIFERASE; MAGNESIUM; PHOSPHATE; PRIMER DNA; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA; TUDOR PROTEIN, DROSOPHILA;

ANIMAL; ARTICLE; CATALYSIS; COMPARATIVE STUDY; DROSOPHILA; ENZYMOLOGY; METABOLISM; PHYSIOLOGY; RNA INTERFERENCE;

ANIMALS; CATALYSIS; DNA PRIMERS; DROSOPHILA; DROSOPHILA PROTEINS; ENDONUCLEASES; LUCIFERASES; MAGNESIUM; MEMBRANE TRANSPORT PROTEINS; PHOSPHATES; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNA-INDUCED SILENCING COMPLEX;

ANIMALIA; MAMMALIA;

EID: 2342626647     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cub.2004.03.008     Document Type: Article

References (23)

1. Tuschl T., Zamore P.D., Lehmann R., Bartel D.P., Sharp P.A. Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev. 13:1999;3191-3197
2. Zamore P.D., Tuschl T., Sharp P.A., Bartel D.P. Rnai. double-stranded rna directs the atp-dependent cleavage of mRNA at 21 to 23 nucleotide intervals Cell. 101:2000;25-33
3. Nykänen A., Haley B., Zamore P.D. ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell. 107:2001;309-321
4. Hutvágner G., Zamore P.D. A MicroRNA in a multiple-turnover RNAi enzyme complex. Science. 297:2002;2056-2060
5. Elbashir S.M., Lendeckel W., Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15:2001;188-200
6. Elbashir S.M., Martinez J., Patkaniowska A., Lendeckel W., Tuschl T. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J. 20:2001;6877-6888
7. Martinez J., Patkaniowska A., Urlaub H., Lührmann R., Tuschl T. Single-stranded antisense siRNA guide target RNA cleavage in RNAi. Cell. 110:2002;563-574
8. Schwarz D.S., Hutvágner G., Haley B., Zamore P.D. Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways. Mol. Cell. 10:2002;537-548
9. Chiu Y.L., Rana T.M. siRNA function in RNAi. a chemical modification analysis RNA. 9:2003;1034-1048
10. Tang G., Reinhart B.J., Bartel D.P., Zamore P.D. A biochemical framework for RNA silencing in plants. Genes Dev. 17:2003;49-63
11. Xie Z., Kasschau K.D., Carrington J.C. Negative feedback regulation of Dicer-Like1 in Arabidopsis by microRNA-guided mRNA degradation. Curr. Biol. 13:2003;784-789
12. Llave C., Xie Z., Kasschau K.D., Carrington J.C. Cleavage of Scarecrow-Like mRNA targets directed by a class of Arabidopsis miRNA. Science. 297:2002;2053-2056
13. Palatnik J.F., Allen E., Wu X., Schommer C., Schwab R., Carrington J.C., Weigel D. Control of leaf morphogenesis by microRNAs. Nature. 425:2003;257-263
14. Holen T., Amarzguioui M., Wiiger M.T., Babaie E., Prydz H. Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. Nucleic Acids Res. 30:2002;1757-1766
15. Caudy A.A., Ketting R.F., Hammond S.M., Denli A.M., Bathoorn A.M., Tops B.B., Silva J.M., Myers M.M., Hannon G.J., Plasterk R.H. A micrococcal nuclease homologue in RNAi effector complexes. Nature. 425:2003;411-414
16. Tomari Y., Du T., Haley B., Schwarz D.S., Bennett R., Cook H.A., Koppetsch B.S., Theurkauf W.E., Zamore P.D. RISC assembly defects in the Drosophila RNAi mutant armitage. Cell. 116:2004;831-841
17. Eckstein F. Nucleoside phosphorothioates. Annu. Rev. Biochem. 54:1985;367-402
18. Pecoraro V.L., Hermes J.D., Cleland W.W. Stability constants of Mg2+ and Cd2+ complexes of adenine nucleotides and thionucleotides and rate constants for formation and dissociation of MgATP and MgADP. Biochemistry. 23:1984;5262-5267
19. Cunningham L., Catlin B.W., Privat de Garilhe M. A deoxyribonuclease of Micrococcus pyogenes. J. Am. Chem. Soc. 78:1956;4642-4645
20. Reddi K.K. Action of micrococcal phosphodiesterase on tobacco mosaic virus nucleic acid. Nature. 182:1958;1308
21. Reddi K.K. Mode of action of micrococcal phosphodiesterase. Nature. 187:1960;74-75
22. Cuatrecasas P., Fuchs S., Anfinsen C.B. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus. J. Biol. Chem. 242:1967;1541-1547
23. Cuatrecasas P., Fuchs S., Anfinsen C.B. The interaction of nucleotides with the active site of staphylococcal nuclease. Spectrophotometric studies. J. Biol. Chem. 242:1967;4759-4767