Double-stranded siRNA targeted to the huntingtin gene does not induce DNA methylation

Biochemical and Biophysical Research Communications

Volumn 323, Issue 1, 2004, Pages 275-280

  Park, Chang Won ^a   Chen, Zongyu ^a   Kren, Betsy T ^a   Steer, Clifford J ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

Bisulfite sequencing; CpG dinucleotides; De novo DNA methylation; Epigenetic modification; Huntington's disease; Neuronal cell culture; Plant genetics; Post transcriptional gene silencing; RNA interference

Indexed keywords

DOUBLE STRANDED RNA; PLASMID VECTOR; SMALL INTERFERING RNA;

ARTICLE; CONTROLLED STUDY; CPG ISLAND; DNA METHYLATION; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; GENOME; GLIOBLASTOMA CELL; HUMAN; HUMAN CELL; HUNTINGTON CHOREA; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA INTERFERENCE; SEQUENCE HOMOLOGY; SOMATIC CELL;

CELL LINE, TUMOR; CPG ISLANDS; DNA METHYLATION; GENE SILENCING; GENETIC VECTORS; GENOME; HUMANS; MODELS, GENETIC; NERVE TISSUE PROTEINS; NEURONS; NUCLEAR PROTEINS; PLASMIDS; RNA; RNA INTERFERENCE; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 4444314502     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.08.096     Document Type: Article

References (26)

1. S. Guo, and K.J. Kemphues Par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed Cell 81 1995 611 620
2. A. Fire, S. Xu, M.K. Montgomery, S.A. Kostas, S.E. Driver, and C.C. Mello Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Nature 391 1998 806 811
3. S.M. Hammond, A.A. Caudy, and G.J. Hannon Post-transcriptional gene silencing by double-stranded RNA Nat. Rev. Genet. 2 2001 110 119
4. N.J. Caplen, S. Parrish, F. Imani, A. Fire, and R.A. Morgan Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems Proc. Natl. Acad. Sci. USA 98 2001 9742 9747
5. S.M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Nature 411 2001 494 498
6. P. Provost, D. Dishart, J. Doucet, D. Frendewey, B. Samuelsson, and O. Radmark Ribonuclease activity and RNA binding of recombinant human Dicer EMBO J. 21 2002 5864 5874
7. H. Kawasaki, E. Suyama, M. Iyo, and K. Taira siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells Nucleic Acids Res. 31 2003 981 987
8. S.M. Hammond, E. Bernstein, D. Beach, and G.J. Hannon An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells Nature 404 2000 293 296
9. M. Matzke, W. Aufsatz, T. Kanno, L. Daxinger, I. Papp, M.F. Mette, and A.J. Matzke Genetic analysis of RNA-mediated transcriptional gene silencing Biochim. Biophys. Acta. 1677 2004 129 141
10. M.F. Mette, W. Aufsatz, J. van der Winden, M. Matzke, and A. Matzke Transcriptional silencing and promoter methylation triggered by double stranded RNA EMBO J. 19 2000 5194 5201
11. T. Sijen, I. Vijn, A. Rebocho, R. van Blokland, D. Roelofs, J. Mol, and J. Kooter Transcriptional and posttranscriptional gene silencing are mechanistically related Curr. Biol. 11 2001 436 440
12. A. Hamilton, O. Voinnet, L. Chappell, and D. Baulcombe Two classes of short interfering RNA in RNA silencing EMBO J. 21 2002 4671 4679
13. S.W. Chan, D. Zilberman, Z. Xie, L.K. Johansen, J.C. Carrington, and S.E. Jacobsen RNA silencing genes control de novo DNA methylation Science 303 2004 1336
14. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl Current Protocols in Molecular Biology 1998 John Wiley and Sons
15. C. Grunau, S.J. Clark, and A. Rosenthal Bisulfite genomic sequencing: systematic investigation of critical experimental parameters Nucleic Acids Res. 29 2001 e65
16. R. Paulin, G.W. Grigg, M.W. Davey, and A.A. Piper Urea improves efficiency of bisulphite-mediated sequencing of 5′-methylcytosine in genomic DNA Nucleic Acids Res. 26 1998 5009 5010
17. M. Frommer, L.E. McDonald, D.S. Millar, C.M. Collis, F. Watt, G.W. Grigg, P.L. Molloy, and C.L. Paul A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands Proc. Natl. Acad. Sci. USA 89 1992 1827 1831
18. T.R. Brummelkamp, R. Bernards, and R. Agami A system for stable expression of short interfering RNAs in mammalian cells Science 296 2002 550 553
19. C. Tufarelli, J.A. Stanley, D. Garrick, J.A. Sharpe, H. Ayyub, W.G. Wood, and D.R. Higgs Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease Nat. Genet. 34 2003 157 165
20. R.I. Verona, M.R. Mann, and M.S. Bartolomei Genomic imprinting: intricacies of epigenetic regulation in clusters Annu. Rev. Cell. Dev. Biol. 19 2003 237 259
21. The Huntington's Disease Collaborative Research Group, A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes, Cell 72 (1993) 971-983
22. L.C. Li, and R. Dahiya MethPrimer: designing primers for methylation PCRs Bioinformatics 18 2002 1427 1431
23. M. Freitag, D.W. Lee, G.O. Kothe, R.J. Pratt, R. Aramayo, and E.U. Selker DNA methylation is independent of RNA interference in Neurospora Science 304 2004 1939
24. P. Svoboda, P. Stein, W. Filipowicz, and R.M. Schultz Lack of homologous sequence-specific DNA methylation in response to stable dsRNA expression in mouse oocytes Nucleic Acids Res. 32 2004 3601 3606
25. V. Schramke, and R. Allshire Hairpin RNAs and retrotransposon LTRs effect RNAi and chromatin-based gene silencing Science 301 2003 1069 1074
26. M. Pal-Bhadra, B.A. Leibovitch, S.G. Gandhi, M. Rao, U. Bhadra, J.A. Birchler, and S.C. Elgin Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery Science 303 2004 669 672