The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK

Journal of Cell Biology

Volumn 166, Issue 4, 2004, Pages 473-478

  Ganem, Neil J ^a   Compton, Duane A ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

Centrosome; Kinetochore; Microtubule; Mitotic spindle; Poleward flux

Indexed keywords

KINESIN; KINESIN EG5; PROTEIN KIF2A; PROTEIN KIN1; PROTEIN MCAK; RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELLULAR DISTRIBUTION; CENTROMERE; CENTROSOME; DEPOLYMERIZATION; HUMAN; HUMAN CELL; MICROTUBULE ORGANIZING CENTER; MITOSIS; MITOSIS SPINDLE; NERVE CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN FUNCTION; REGULATORY MECHANISM;

ANIMALS; CELL CYCLE; CELL LINE, TUMOR; CHO CELLS; CRICETINAE; DISEASE PROGRESSION; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HELA CELLS; HUMANS; KINESIN; KINETOCHORES; MICROTUBULES; MITOSIS; MITOTIC SPINDLE APPARATUS; NERVE TISSUE PROTEINS; NEURONS; NOCODAZOLE; RANIDAE; REPRESSOR PROTEINS; RNA INTERFERENCE; TRANSFECTION;

ANIMALIA;

EID: 4143075910     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200404012     Document Type: Article

References (42)

1. Cassimeris, L., and J. Morabito. 2004. TOGp, the human homolog of XMAP215/ Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly. Mol. Biol. Cell. 15:1580-1590.
2. Compton, D.A. 2000. Spindle assembly in animal cells. Annu. Rev. Biochem. 69: 95-114.
3. Cottingham, F.R., L. Gheber, D.L. Miller, and M.A. Hoyt. 1999. Novel roles for Saccharomyces cerevisiae mitotic spindle motors. J. Cell Biol. 147:335-350.
4. Debernardi, S., E. Fontanella, L. De Gregorio, M.A. Pierotti, and D. Delia. 1997. Identification of a novel human kinesin-related gene (HK2) by the cDNA differential display technique. Genomics. 42:67-73.
5. DeLuca, J.G., B.J. Howell, J.C. Canman, J.M. Hickey, G. Fang, and E.D. Salmon. 2003. Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Curr. Biol. 13:2103-2109.
6. Desai, A., S. Verma, T.J. Mitchison, and C.E. Walczak. 1999. Kin I kinesins are microtubule-destabilizing enzymes. Cell. 96:69-78.
7. Elbashir, S.M., J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:494-498.
8. Endow, S.A., R. Chandra, D.J. Domma, A.H. Yamamoto, and E.D. Salmon. 1994. Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle defects in mitosis. J. Cell Sci. 107:859-867.
9. Gaetz, J., and M. Kapoor. 2004. Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles. J. Cell Biol. 166:465-471.
10. Goshima, G., and R.D. Vale. 2003. The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line. J. Cell Biol. 162:1003-1016.
11. Holmfeldt, P., S. Stenmark, and M. Gullberg. 2004. Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis. EMBO J. 23:627-637.
12. Homma, N., Y. Takei, Y. Tanaka, T. Nakata, S. Terada, M. Kikkawa, Y. Noda, and N. Hirokawa. 2003. Kinesin superfamily protein 2A (KIF2A) functions in suppression of collateral branch extension. Cell. 114:229-239.
13. Hunter, A.W., M. Caplow, D.L. Coy, W.O. Hancock, S. Diez, L. Wordeman, and J. Howard. 2003. The kinesin-related protein MCAK is a microtubule depolymerase that forms an ATP-hydrolyzing complex at microtubule ends. Mol. Cell. 11:445-457.
14. Hyman, A.A., and E. Karsenti. 1996. Morphogenetic properties of microtubules and mitotic spindle assembly. Cell. 84:401-410.
15. Inoue, S., and E.D. Salmon. 1995. Force generation by microtubule assembly/disassembly in mitosis and related movements. Mol. Biol. Cell. 6:1619-1640.
16. Jordan, M.A., D. Thrower, and L. Wilson. 1992. Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis. J. Cell Sci. 102:401-416.
17. Kinoshita, K., I. Arnal, A. Desai, D.M. Drechsel, and A.A. Hyman. 2001. Reconstitution of physiological microtubule dynamics using purified components. Science. 294:1340-1342.
18. Kline-Smith, S.L., and C.E. Walczak. 2002. The microtubule-destabilizing kinesin XKCM1 regulates microtubule dynamic instability in cells. Mol. Biol. Cell. 13:2718-2731.
19. Kline-Smith, S.L., A. Khodjakov, P. Hergert, and C.E. Walczak. 2004. Depletion of centromeric MCAK leads to chromosome congression and segregation defects due to improper kinetochore attachments. Mol. Biol. Cell. 15:1146-1159.
20. Levesque, A.A., and D.A. Compton. 2001. The chromokinesin Kid is necessary for chromosome arm orientation and oscillation, but not congression, on mitotic spindles. J. Cell Biol. 154:1135-1146.
21. Mack, G.J., and D.A. Compton. 2001. Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein. Proc. Natl. Acad. Sci. USA. 98:14434-14439.
22. Maddox, P., A. Straight, P. Coughlin, T.J. Mitchison, and E.D. Salmon. 2003. Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics. J. Cell Biol. 162:377-382.
23. Maney, T., A.W. Hunter, M. Wagenbach, and L. Wordeman. 1998. Mitotic centromere-associated kinesin is important for anaphase chromosome segregation. J. Cell Biol. 142:787-801.
24. Maney, T., M. Wagenbach, and L. Wordeman. 2001. Molecular dissection of the microtubule depolymerizing activity of mitotic centromere-associated kinesin. J. Biol. Chem. 276:34753-34758.
25. Mayer, T.U., T.M. Kapoor, S.J. Haggarty, R.W. King, S.L. Schreiber, and T.J. Mitchison. 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science. 286:971-974.
26. McIntosh, J.R., and M.P. Koonce. 1989. Mitosis. Science. 246:622-628.
27. Moores, C.A., M. Yu, J. Guo, C. Beraud, R. Sakowicz, and R.A. Milligan. 2002. A mechanism for microtubule depolymerization by KinI kinesins. Mol. Cell. 9:903-909.
28. Morfini, G., S. Quiroga, A. Rosa, K. Kosik, and A. Caceres. 1997. Suppression of KIF2 in PC12 cells alters the distribution of a growth cone nonsynaptic membrane receptor and inhibits neurite extension. J. Cell Biol. 138:657-669.
29. Mountain, V., C. Simerly, L. Howard, A. Ando, G. Schatten, and D.A. Compton. 1999. The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle. J. Cell Biol. 147: 351-366.
30. Noda, Y., R. Sato-Yoshitake, S. Kondo, M. Nangaku, and N. Hirokawa. 1995. KIF2 is a new microtubule-based anterograde motor that transports membranous organelles distinct from those carried by kinesin heavy chain or KIF3A/B. J. Cell Biol. 129:157-167.
31. Ogawa, T., R. Nitta, Y. Okada, and N. Hirokawa. 2004. A common mechanism for microtubule destabilizers-M type kinesins stabilize curling of the protofilament using the class-specific neck and loops. Cell. 116:591-602.
32. Ohi, R., M.L. Coughlin, W.S. Lane, and T.J. Mitchison. 2003. An inner centromere protein that stimulates the microtubule depolymerizing activity of a KinI kinesin. Dev. Cell. 5:309-321.
33. Rogers, G.G., S.L. Rogers, T.A. Schwimmer, S.C. Ems-McClung, C.E. Walczak, R.D. Vale, J.M. Scholey, and D.J. Sharp. 2003. Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature. 427:364-370.
34. Sawin, K.E., K. LeFuellec, M. Philippe, and T.J. Mitchison. 1992. Mitotic spindle organization by a plus-end-directed microtubule motor. Nature. 359:540-543.
35. Sharp, D.J., G.C. Rogers, and J.M. Scholey. 2000. Microtubule motors in mitosis. Nature. 407:41-47.
36. Tournebize, R., A. Popov, K. Kinoshita, A.J. Ashford, S. Rybina, A. Pozniakovsky, T.U. Mayer, C.E. Walczak, E. Karsenti, and A.A. Hyman. 2000. Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. Nat. Cell Biol. 2:13-19.
37. Vale, R.D., and R.J. Fletterick. 1997. The design plan of kinesin motors. Annu. Rev. Cell Dev. Biol. 13:745-777.
38. Vasquez, R.J., B. Howell, A.M. Yvon, P. Wadsworth, and L. Cassimeris. 1997. Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol. Biol. Cell. 8:973-985.
39. Walczak, C.E., T.J. Mitchison, and A. Desai. 1996. XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell. 84:37-47.
40. Walczak, C.E., E.G. Gan, A. Desai, T.J. Mitchison, and S.L. Kline-Smith. 2002. The microtubule-destabilizing kinesin XKCM1 is required for proper chromosome positioning during spindle assembly. Curr. Biol. 12:1885-1889.
41. Wordeman, L., and T.J. Mitchison. 1995. Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis. J. Cell Biol. 128:95-104.
42. Yao, X., K.L. Anderson, and D.W. Cleveland. 1997. The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules. J. Cell Biol. 139:435-447.