Dual-fluorophore quantitative high-throughput screen for inhibitors of BRCT-phosphoprotein interaction

Analytical Biochemistry

Volumn 375, Issue 1, 2008, Pages 60-70

  Simeonov, Anton ^a   Yasgar, Adam ^a   Jadhav, Ajit ^a   Lokesh, G L ^b   Klumpp, Carleen ^a   Michael, Sam ^a   Austin, Christopher P ^a   Natarajan, Amarnath ^b   Inglese, James ^a  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^b University of Texas Medical Branch School of Medicine   (United States)

Author keywords

1536 Well plate; BRCT; Fluorescence polarization assay; Hotspot interaction; pBACH1; Quantitative high throughput screening

Indexed keywords

DIAGNOSIS; FLUORESCENCE; FLUOROPHORES; MOLECULES; POLARIZATION; TUMORS;

BRCT; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; HOT SPOT; PBACH1;

PROTEINS;

AMINO ACID; BRCA1 PROTEIN; FLUORESCEIN; PHOSPHOPROTEIN; PROTEIN ABRAXAS; PROTEIN BACH1; PROTEIN BRCT; PROTEIN CTIP; RHODAMINE; UNCLASSIFIED DRUG;

ARTICLE; ARTIFACT; COMPLEX FORMATION; DNA DAMAGE; DNA REPAIR; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS;

EID: 39749162335     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.11.039     Document Type: Article

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