The effect of reciprocal active site mutations in human cytochromes P450 1A1 and 1A2 on alkoxyresorufin metabolism

Archives of Biochemistry and Biophysics

Volumn 424, Issue 1, 2004, Pages 33-43

  Liu, Jianguo ^a   Ericksen, Spencer S ^a   Sivaneri, Meena ^a   Besspiata, Dan ^a   Fisher, Charles W ^b,c   Szklarz, Grazyna D ^a  

^a WEST VIRGINIA UNIVERSITY   (United States)

^b TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^c CEDRA Corporation   (United States)

Author keywords

Alkoxyresorufin O dealkylation; Cytochrome P450; Molecular dynamics; Molecular modeling; Site directed mutagenesis; Structure function relationships; Substrate specificity

Indexed keywords

ALKOXYRESORUFIN; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; MUTANT PROTEIN; NICKEL; NITRILOTRIACETIC ACID; RESORUFIN DERIVATIVE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; ESCHERICHIA COLI; METABOLISM; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; WILD TYPE;

AMINO ACID SUBSTITUTION; BINDING SITES; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP1A2; DEALKYLATION; ESCHERICHIA COLI; HUMANS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXAZINES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI;

EID: 1542328873     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.abb.2003.12.040     Document Type: Article

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