High level production and purification of human interferon α2b in high cell density culture of Pichia pastoris

Enzyme and Microbial Technology

Volumn 42, Issue 2, 2008, Pages 173-180

  Ayed, Atef ^a   Rabhi, Imen ^a   Dellagi, Koussay ^a   Kallel, Héla ^a  

^a PASTEUR INSTITUTE OF TUNIS   (Tunisia)

Author keywords

Antiviral activity; High cell density culture; Human interferon 2b; Pichia pastoris; Purification process

Indexed keywords

BIOACTIVITY; CELL CULTURE; CLONING; ION EXCHANGE; METHANOL; MICROFILTRATION; PROTEOLYSIS;

ANTIVIRAL ACTIVITY; HIGH CELL DENSITY CULTURE; PICHIA PASTORIS;

INTERFERONS;

ALCOHOL OXIDASE; CASAMINO ACID; EDETIC ACID; GLYCEROL; METHANOL; PROTEINASE; SEPHAROSE; SODIUM CHLORIDE; TRITON X 100;

ARTICLE; BIOREACTOR; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; FUNGUS CULTURE; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION; HUMAN; HUMAN INTERFERON ALPHA2B GENE; MICROFILTRATION; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PROTEIN TARGETING; RECOMBINANT GENE;

PICHIA PASTORIS;

EID: 36849034496     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2007.09.006     Document Type: Article

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