Interfacing Pichia pastoris cultivation with expanded bed adsorption

Biotechnology and Bioengineering

Volumn 93, Issue 6, 2006, Pages 1040-1049

  Jahic, Mehmedalija ^a   Knoblechner, Josef ^a   Charoenrat, Theppanya ^a   Enfors, Sven Olof ^a   Veide, Andres ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Expanded bed adsorption; Fed batch processes; Pichia pastoris

Indexed keywords

ADSORPTION; CELLULOSE; CULTIVATION; PROTEINS; VISCOSITY;

EXPANDED BED ADSORPTION; FED-BATCH PROCESSES; PICHIA PASTORIS;

CROPS;

CELLULASE; LIPASE B; SODIUM CHLORIDE;

ARTICLE; CANDIDA ANTARCTICA; CELL DEATH; CELL DENSITY; CONDUCTANCE; CONTROLLED STUDY; ENZYME ACTIVITY; EXPANDED BED ADSORPTION; FLOW CYTOMETRY; FLOW RATE; FUNGUS CULTURE; NEOCALLIMASTIX; NONHUMAN; PICHIA PASTORIS; PROTEIN DEGRADATION; SUPERNATANT; VISCOSITY;

BIOMASS; BIOREACTORS; CELL DIVISION; CELLULASE; CHROMATOGRAPHY, GEL; CULTURE MEDIA; HYDROGEN-ION CONCENTRATION; LIPASE; MYCOLOGY; NEOCALLIMASTIX; OSMOLAR CONCENTRATION; PICHIA; RECOMBINANT FUSION PROTEINS;

ADSORPTION; CELLULOSE; CULTIVATION; FARM CROPS; PROTEINS; VISCOSITY;

CANDIDA ANTARCTICA; FUNGI; NEOCALLIMASTIX; NEOCALLIMASTIX PATRICIARUM; PICHIA PASTORIS;

EID: 33646045883     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20811     Document Type: Article

References (29)

1. Anspach FB, Curbelo D, Hartmann R, Garke G, Deckwer W-D. 1999. Expanded-bed chromatography in primary protein purification. J Chrom 865(1+2):129-144.
2. Axelsson H. 1999. In: Flickinger MC, Drew SW, editors. Encyclopedia of bioprocess technology: Fermentation, biocatalysis, and bioseparation. New York: John Wiley & Sons, Inc. 513 p.
3. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochem 72:248-254.
4. Brady CP, Shimp RL, Miles AP, Whitmore M, Stowers AW. 2001. High-level production and purification of P30P2MSP119, an important vaccine antigen for malaria, expressed in the methylotropic yeast Pichia pastoris. Protein Expr Purif 23(3):468-475.
5. Cereghino LN, Geoffrey P, Sunga AJ, Lin Cereghino J, Cregg JM. 2001. Expression of foreign genes in the yeast Pichia pastoris. Genet Eng 23:157-169.
6. Cereghino GPL, Cereghino JL, Ilgen C, Cregg JM. 2002. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13(4):329-332.
7. Chang YK, Chase HA. 1996. Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance. Biotechnol Bioeng 49(5):512-526.
8. Charoenrat T, Ketudat-Cairns M, Jahic M, Enfors SO, Veide A. Recovery of recombinant-glucosidase by expanded bed adsorption from Pichia pastoris high-cell-density culture broth. J Biotechnol In press.
9. Digan EM, Tschoop J, Grinna L, Lair VS, Craig SW, Velicelebi G, Siegel R, Davis RG, Thill PG. 1988. Secrition of heterologous proteins from the methylotrophic yeast, Pichia pastoris. Ind Microbiol 29:59-65.
10. Fernandez-Lahore HM, Geilenkirchen S, Boldt K, Nagel A, Kula M-R, Thommes J. 2000. The influence of cell adsorbent interactions on protein adsorption in expanded beds. J Chrom 837(2):195-208.
11. Feuser J, Walter J, Kula MR, Thommes J. 1999. Cell/adsorbent interactions in expanded bed adsorption of proteins. Biosep 8(1-5):99-109.
12. Gustavsson M, Lehtio J, Denman S, Teeri TT, Hult K, Martinelle M. 2001. Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris. Protein Eng 14(9):711-715.
13. Hjorth R. 1997. Expanded-bed adsorption in industrial bioprocessing: Recent developments. Trends Biotechnol 15(6):230-235.
14. Hong F, Meinander NQ, Jonsson LJ. 2002. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol Bioeng 79(4):438-449.
15. Hubbuch JJ, Heebøll-Nielsen A, Hobley TJ, Thomas ORT. 2002. A new fluid distribution system for scale-flexible expanded bed adsorption. Biotechnol Bioeng 78(1):35-43.
16. Inan M, Chiruvolu V, Eskridge KM, Vlasuk GP, Dickerson K, Brown S, Meagher MM. 1999. Optimization of temperature-glycerol-pH conditions for a fed-batch fermentation process for recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (AcAP-5) production by Pichia pastoris. Enzyme Microb Technol 24(7):438-445.
17. Jahic M, Gustavsson M, Jansen AK, Martinelle M, Enfors S-O. 2003a. Analysis and control of proteoly sis of a fusion protein in Pichia pastoris fed-batch process. J Biotechnol 102:45-53.
18. Jahic M, Wallberg F, Bollok M, Garcia P, Enfors S-O. 2003b. Temperature limited fed-batch technique for control of proteoly sis in Pichia pastoris bioreactor cultures. Microb Cell Fact 2(1):1-11. http://www.microbial-cellfactories.com/content/2/1/6.
19. Jones EW. 1990. Vacuolar proteases in yeast Saccharomyces cerevisiae. Methods Enzymol 185:372-386.
20. Jones EW. 2002. Vacuolar proteases and proteolytic artifacts in Saccharomyces cerevisiae. Methods Enzymol 351:127-150.
21. Li Z, Xiong F, Lin Q, d'Anjou M, Daugulis AJ, Yang DSC, Hew CL. 2001. Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris. Protein Expr Purif 21(3):438-445.
22. Lin D-Q, Thoemmes J, Kula M-R, Hubbuch JJ. 2004. The influence of biomass on the hydrodynamic behavior and stability of expanded beds. Biotechnol Bioeng 87(3):337-346.
23. Rotticci-Mulder JC, Gustavsson M, Holmquist M, Hult K, Martinelle M. 2001. Expression in Pichia pastoris of Candida antarctica lipase B and lipase B fused to a cellulose-binding domain. Protein Expr Purif 21:386-392.
24. Shepard SR, Boucher R, Johnston J, Boerner R, Koch G, Madsen JW, Grella D, Sim BKL, Schrimsher JL. 2000. Large-scale purification of recombinant human angiostatin. Protein Expr Purif 20(2):216-227.
25. Shepard SR, Boyd GA, Schrimsher JL. 2001. Routine manufacture of recombinant proteins using expanded bed adsorption chromatography. Biosep 10(1/3):51-56.
26. Thoemmes J, Halfar M, Gieren H, Curvers S, Takors R, Brunschier R, Kula M-R. 2001. Human chymotrypsinogen B production from Pichia pastoris by integrated development of fermentation and downstream processing. Part 2. Protein recovery. Biotechnol Prog 17(3):503-512.
27. Trinh L, Noronha SB, Fannon M, Shiloach J. 2000. Recovery of mouse endostatin produced by Pichia pastoris using expanded bed adsorption. Biosep 9(4):223-230.
28. Wegner EH. 1983. Biochemal conversions by yeast fermentation at high cell densities. US patent 316164.
29. Zhou X-S, Zhang Y-X. 2002. Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnol Lett 24(17):1449-1453.