The inhibition of aggregation of recombinant human consensus interferon-α mutant during Pichia pastoris fermentation

Applied Microbiology and Biotechnology

Volumn 74, Issue 3, 2007, Pages 578-584

  Hao, Yuyou ^a   Chu, Ju ^a   Wang, Yonghong ^a   Zhuang, Yingping ^a   Zhang, Siliang ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Aggregation; Consensus interferon ; Pichia pastoris; Temperature; Tween 20

Indexed keywords

BIOCHEMISTRY; CELL CULTURE; GENETIC ENGINEERING; MONOMERS; MUTAGENESIS; TEMPERATURE; YEAST;

CONSENSUS INTERFERON-Α; PICHIA PASTORIS; SORBITAN MONOLAURATE; TWEEN-20;

FERMENTATION;

MONOMER; MUTANT PROTEIN; POLYSORBATE 20; RECOMBINANT ALPHA INTERFERON;

AGGREGATION; FERMENTATION; LOW TEMPERATURE; PROTEIN; RECOMBINATION; SURFACTANT; YEAST;

ARTICLE; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; FERMENTATION TECHNIQUE; NONHUMAN; PICHIA PASTORIS; PROTEIN AGGREGATION; PROTEIN SECRETION; TEMPERATURE DEPENDENCE;

DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; INTERFERON TYPE I, RECOMBINANT; MUTATION; PICHIA; POLYSORBATES; TEMPERATURE;

PICHIA PASTORIS;

EID: 33847146241     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0715-9     Document Type: Article

References (22)

1. Alton K, Stabinsky Y, Richards R, Ferguson B, Goldstein L, Altrock B, Miller L, Stebbing N (1983) Production, characterization and biological effects of recombinant DNA derived human IFN-α and IFNγ analogs. In: De Maeyer E, Schellekens H (eds) Biology of the interferon system. Elservier, Amsterdam, pp 119-128
2. Bitter GA, Chen KK, Banks AR, Lai P-H (1984) Secretion of foreign proteins from Saccharomyces cerevisiae directed by α-factor gene fusions. Proc Natl Acad Sci U S A 81:5330-5334
3. Curless C, Pope J, Tsai L (1990) Effect of preinduction specific growth rate on recombinant alpha consensus interferon synthesis in Escherichia coli. Biotechnol Prog 6:149-152
4. Daly R, Heart MTW (2005) Expression of heterologous proteins in Pichia pastoris, a useful experimental tool in protein engineering and production. J Mol Recognit 18:119-138
5. Fieschko J, Ritch T (1986) Production of human alpha consensus interferon in recombinant Escherichia coli. Chem Eng Commun 45:229-240
6. Hao YY, Chu J, Wang YH, Zhang SL, Zhuang YP (2006) Expression and aggregation of recombinant human consensus interferon-α mutant by Pichia pastoris. Biotechnol Lett 28(12):905-909
7. Hong F, Meinander NQ, Jönsson LJ (2002) Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol Bioeng 79:438-449
8. Hou YD (1985) Principle and methods of viral genetic engineering. People's Health Press, Beijing (in Chinese)
9. Huang SL, Lin FY, Yang CP (2005) Microcalorimetric studies of the effects on the interactions of human recombinant interferon-alpha 2a. Eur J Pharm Sci 24(5):545-552
10. Jahic M, Gustavsson M, Jansen A-K, Martinelle M, Enfors S-O (2003) Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J Biotechnol 102:45-53
11. Kreilgaard L, Jones LS, Randolph TW, Frokhaer R, Flink JM, Manning MC, Carpenter JF (1998) Effect of Tween 20 on freeze- thawing- and agitation-induced aggregation of recombinant human factor XIII. J Pharm Sci 87:1597-1603
12. Liu ZM, Zhao HL, Xue C, Yang SZ, Wu KH (2002) Expression and production of human consensus interferon-α mutant. Chinese Patent CN1435486A, pp 33 (in Chinese)
13. Klein ML, Bartley TD, Lai PH, Lu HS (1988) Structural characterization of recombinant consensus interferon-α. J Chromatogr 454:205-216
14. Macauley-Patrick S, Fazenda ML, Mcneil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22:249-270
15. Ozes ON, Reiter Z, Kelin S, Blatt LM, Taylor MW (1992) A comparison of interferon-Con1 with natural recombinant interferon-α: antiviral, antiproliferative and natural killer inducing activities. J Interf Res 12:55-59
16. Sinha J, Plantz B, Inan M, Meagher MM (2005) Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: case study with recombinant ovine interferon-τ. Biotechnol Bioeng 89:102-112
17. Stratton J, Chiruvolu V, Meagher M (1998) High cell-density fermentation. In: Higgins DR, Cregg JM (eds) Methods in molecular biology, vol. 103. Humana, Totowa, NJ, pp 107-120
18. Villaverde A, Carrió MM (2003) Protein aggregation in recombinant bacteria: biological role of inclusion bodies. Biotechnol Lett 25:1385-1395
19. Wang W (1999) Instability, stabilization, and formulation of liquid protein pharmaceuticals. Int J Pharm 185:129-188
20. Wang JZ, Fan M (2000) Manual of protein technique. Science Press, Beijing (in Chinese)
21. Wang HH, Gao BY, Chen PL, Dong L, Li YY (1995) Analysis of heterogeneity of gene products (interferon) expressed in yeast. Sci China Ser B Chem Life Sci Earth Sci 38:954-962
22. Yu CM, Fu L, Qi LQ, Zhang XP, Yu T, Lai DZ, Wang HT, Chen W (2003) Expression, purification and activity analysis of consensus interferon mutant produced in Pichia pastoris. Chin J Biochem Mol Biol 19:317-320