Characterization of rat and mouse NAD+-dependent 3α/17β/20α-hydroxysteroid dehydrogenases and identification of substrate specificity determinants by site-directed mutagenesis

Archives of Biochemistry and Biophysics

Volumn 467, Issue 1, 2007, Pages 76-86

  Endo, Satoshi ^a   Sanai, Masaharu ^a   Horie, Kenji ^a   Matsunaga, Toshiyuki ^a   Ishikura, Shuhei ^a   Tajima, Kazuo ^b   El Kabbani, Ossama ^c   Hara, Akira ^a  

^a GIFU PHARMACEUTICAL UNIVERSITY   (Japan)

^b HOKURIKU UNIVERSITY   (Japan)

^c MONASH UNIVERSITY   (Australia)

Author keywords

3 Hydroxysteroid dehydrogenase; 3 17 20 Hydroxysteroid dehydrogenase; Aldo keto reductase superfamily; Site directed mutagenesis; Steroid specificity

Indexed keywords

20ALPHA HYDROXYSTEROID DEHYDROGENASE; 3ALPHA HYDROXYSTEROID DEHYDROGENASE; AMINO ACID; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; TESTOSTERONE 17BETA DEHYDROGENASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FEMALE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SITE DIRECTED MUTAGENESIS; TISSUE DISTRIBUTION;

ALCOHOLS; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; ESTRADIOL DEHYDROGENASES; HYDROXYSTEROID DEHYDROGENASES; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RATS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY; SUBSTRATE SPECIFICITY; TISSUE DISTRIBUTION; TYROSINE;

RATTUS;

EID: 35548966188     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2007.08.011     Document Type: Article

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