Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics

Journal of Structural and Functional Genomics

Volumn 5, Issue 1-2, 2004, Pages 29-44

  Prinz, Bianka ^a,b   Schultchen, Jeffrey ^b   Rydzewski, Ralf ^b   Holz, Caterina ^b   Boettner, Mewes ^b   Stahl, Ulf ^a   Lang, Christine ^a,b  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b Proteinstrukturfabrik   (Germany)

Author keywords

Fermentation; Recombinant protein purification; Tagged human proteins; Yeast

Indexed keywords

BUFFER; CARBON; COMPLEMENTARY DNA; GLYCEROL; HISTIDINE; HYBRID PROTEIN; METAL; METHANOL; PROTEIN; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; BATCH FERMENTATION; BIOMASS; BIOREACTOR; CARBOXY TERMINAL SEQUENCE; CHROMATOGRAPHY; CONTROLLED STUDY; COST BENEFIT ANALYSIS; CRYSTALLIZATION; EUKARYOTE; FED BATCH FERMENTATION; FERMENTATION; FUNGAL STRAIN; GEL FILTRATION; GENE EXPRESSION; GENE STRUCTURE; GENOME ANALYSIS; GENOMICS; HOST; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; SCREENING; STRUCTURE ANALYSIS; YEAST;

CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; DNA, COMPLEMENTARY; DNA, RECOMBINANT; FERMENTATION; GENE EXPRESSION; HUMANS; PICHIA; PROTEOMICS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 3543109683     PISSN: 1345711X     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:JSFG.0000029207.13959.90     Document Type: Article

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