A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae

Protein Expression and Purification

Volumn 25, Issue 3, 2002, Pages 372-378

  Holz, Caterina ^a   Hesse, Oliver ^a   Bolotina, Natalia ^a   Stahl, Ulf ^a   Lang, Christine ^a  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BASE SEQUENCE; DNA, COMPLEMENTARY; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

ESCHERICHIA COLI; EUKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036415243     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00029-3     Document Type: Article

References (14)

1. M. Schena, D. Shalon, R.W. Davis, P.R. Brown, Quantitative monitoring of gene expression patterns with a complementary DNA microarray, Science 270 (1995) 467-470
2. D.J. Duggan, M. Bittner, Y. Chen, P. Meltzer, J.M. Trent, Expressing profiling using cDNA microarrays, Nature Genet. 21 (1999) 10-14
3. K. Buessow, D. Cahill, V. Nietfeld, D. Bancroft, E. Scherzinger, H. Lehrach, G. Walter, A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library, Nucleic Acids Res. 26 (1998) 5007-5008
4. S. Hua, Y. Luo, M. Qui, E. Chan, H. Zhou, L. Zhu, Construction of a modular yeast two-hybrid cDNA library from human EST clones of the human genome protein linkage map, Gene 215 (1998) 143-152
5. M.A. Romanos, C.A. Scorer, J.J. Clare, Foreign gene expression in yeast: A review, Yeast 8 (1992) 423-488
6. C. Holz, A. Lueking, L. Bovekamp, C. Gutjahr, N. Bolotina, H. Lehrach, D.J. Cahill, A human cDNA expression library in yeast enriched for open reading frames, Genome Res. 11 (2001) 1730-1735
7. B. Loddenkötter, B. Kammerer, K. Fischer, U.-I. Flugge, Expression of the functional mature chloroplast triose phospate translocator in yeast internal membranes and the purification of the His-tagged protein by a single metal affinity chromatography step, Proc. Natl. Acad. Sci. USA 90 (1998) 2155
8. T.G.M. Schmidt, J. Koepke, R. Frank, A. Skerra, Molecular interaction between the Strep-tag affinity peptide and its cognate target streptavidin, J. Mol. Biol. 255 (1996) 753-766
9. T. Polakowski, U. Stahl, C. Lang, Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast, Appl. Microbiol. Biotechnol. 49 (1998) 66-71
10. D. Gietz, A. St. Jean, R.A. Woods, R.H. Schiestl, Improved method for high efficiency transformation of intact yeast cells, Nucleic Acids Res. 20 (1992) 1425
11. C. Lang, A.C. Looman, Efficient expression and secretion of Aspergillus niger RH5344 polygalacturonase in Saccharomyces cerevisiae, Appl. Microbiol. Biotechnol. 44 (1995) 147-156
12. S. Voss, A. Skerra, Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification, Protein Eng. 10 (1997) 975-982
13. T. Etcheverry, Induced expressing using yeast copper metallothionein promoter, Meth. Enzymol. 185 (1990) 319-329
14. A. Torreggiani, G. Fini, The binding of biotin analogues by streptavidin: A Raman spectroscopic study, Biospectroscopy 4 (1998) 197-208