Purification of histidine-tagged mitochondrial ADP/ATP carrier: Influence of the conformational states of the C-terminal region

Protein Expression and Purification

Volumn 19, Issue 1, 2000, Pages 57-65

  Fiore, Christelle ^a   Trézéguet, Véronique ^b   Roux, Pierre ^a   Le Saux, Agnès ^b   Noël, Florence ^a   Schwimmer, Christine ^b   Arlot, Delphine ^a   Dianoux, Anne Christine ^a   Lauquin, Guy J M ^b   Brandolin, Gérard ^a  

^a CEA GRENOBLE   (France)

^b Institut de Biochimie et Genetique Cellulaires   (France)

Author keywords

ADP ATP carrier; Conformational state; His tag; Protein purification; Saccharomyces cerevisiae

Indexed keywords

ADENOSINE DIPHOSPHATE CARRIER PROTEIN; ADENOSINE TRIPHOSPHATE CARRIER PROTEIN; ADSORPTION; AFFINITY CHROMATOGRAPHY; BINDING SITE; BONGKREKIC ACID; BUFFER; CARBOXY TERMINAL SEQUENCE; CARBOXYATRACTYLOSIDE; ERGOSTEROL; FLUOROMETRY; IMIDAZOLE; MITOCHONDRION; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TAGGING; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0342748712     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1213     Document Type: Article

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