PET imaging of herpes simplex virus type 1 thymidine kinase (HSV1-tk) or mutant HSV1-sr39tk reporter gene expression in mice and humans using [18F]FHBG

Nature Protocols

Volumn 1, Issue 6, 2007, Pages 3069-3075

  Yaghoubi, Shahriar S ^a   Gambhir, Sanjiv S ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

9 (4 ((18)F)FLUORO 3 HYDROXYMETHYLBUTYL)GUANINE; 9-(4-((18)F)FLUORO-3-HYDROXYMETHYLBUTYL)GUANINE; DIAGNOSTIC AGENT; DRUG DERIVATIVE; GUANINE; THYMIDINE KINASE; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ANIMAL; ARTICLE; GENE EXPRESSION; GENETIC PROCEDURES; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOUSE; MUTATION; POSITRON EMISSION TOMOGRAPHY; REPORTER GENE;

ANIMALS; GENE EXPRESSION; GENES, REPORTER; GUANINE; HUMANS; MICE; MOLECULAR PROBE TECHNIQUES; MUTATION; POSITRON-EMISSION TOMOGRAPHY; THYMIDINE KINASE; VIRAL PROTEINS;

EID: 34548864644     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.459     Document Type: Article

References (47)

1. Yaghoubi, S.S. & Gambhir., S.S. Measuring herpes simplex virus thymidine kinase reporter gene expression in vitro. Nat. Protocols. 1, 2137-2142 (2006).
2. Saito, Y. et al. Quantitative autoradiographic mapping of herpes simplex virus encephalitis with radiolabeled antiviral drug. Science 217, 1151-1153 (1982).
3. Tjuvajev, J.G. et al. Noninvasive imaging of herpes virus thymidine kinase gene transfer and expression: A potential method for monitoring clinical gene therapy. Cancer Res. 56, 4087-4095 (1996).
4. Gambhir, S.S. et al. Imaging of adenoviral-directed herpes simplex virus type 1 thymidine kinase reporter gene expression in mice with radiolabeled ganciclovir. J. Nucl. Med. 39, 2003-2011 (1998).
5. Ponomarev, V. et al. A novel triple-modality reporter gene for whole-body fluorescent, bioluminescent, and nuclear noninvasive imaging. Eur. J. Nucl. Med. Mol. Imaging 31, 740-751 (2004).
6. Jacobs, A. et al. Positron-emission tomography of vector-mediated gene expression in gene therapy for gliomas. Lancet 358, 727-729 (2001).
7. Jacobs, A. et al. Positron emission tomography-based imaging of transgene expression mediated by replication-conditional, oncolytic herpes simplex virus type 1 mutant vectors in vivo. Cancer Res. 61, 2983-2995 (2001).
8. Gambhir, S.S. et al. Imaging adenoviral-directed reporter gene expression in living animals with positron emission tomography. Proc. Natl. Acad. Sci. USA 96, 2333-2338 (1999).
9. 18F]fluoropenciclovir: An improved reporter probe for imaging HSV1-tk reporter gene expression in vivo using PET. J. Nucl. Med. 42, 96-105 (2001).
10. Gambhir, S.S. et al. A Mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography. Proc. Natl. Acad. Sci. USA 97, 2785-2790 (2000).
11. Gambhir, S.S. et al. Imaging transgene expression with radionuclide imaging technologies. Neoplasia 2, 118-138 (2000).
12. 18F]FHBG): A new potential imaging agent for viral infection and gene therapy using PET. Nucl. Med. Biol. 25, 175-180 (1998).
13. 18F]fluoro-3-hydroxymethylbutyl)guanine for in vivo measurement of suicide gene expression with PET. J. Nucl. Med. 42, 1682-1690 (2001).
14. Black, M.E., Kokoris, M.S. & Sabo, P. Herpes simplex virus-1 thymidine kinase mutants created by semi-random sequence mutagenesis improve prodrug-mediated tumor cell killing. Cancer Res. 61, 3022-3026 (2001).
15. Black, M.E., Newcomb, T.G., Wilson, H.M. & Loeb, L.A. Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proc. Natl. Acad. Sci. USA 93, 3525-3529 (1996).
16. 14C]FIAU for imaging of HSV1-tk reporter gene expression: Adenoviral infection vs stable transfection. Eur. J. Nucl. Med. Mol. Imaging 30, 1547-1560 (2003).
17. Tjuvajev, J.G. et al. Comparison of radiolabeled nucleoside probes (FIAU, FHBG, and FHPG) for PET imaging of HSV1-tk gene expression. J. Nucl. Med. 43, 1072-1083 (2002).
18. Alauddin, M.M., Shahinian, A., Gordon, E.M. & Conti, P.S. Direct comparison of radiolabeled probes FMAU, FHBG, and FHPG as PET imaging agents for HSV1-tk expression in a human breast cancer model. Mol. Imaging 3, 76-84 (2004).
19. 18F-FEAU as a radiotracer for herpes simplex virus thymidine kinase gene expression: in-vitro comparison with other PET tracers. Nucl. Med. Commun. 27, 25-30 (2006).
20. 18F]FHBG: A reporter probe for imaging herpes simplex virus type-1 thymidine kinase reporter gene expression. J. Nucl. Med. 42, 1225-1234 (2001).
21. 18F-FHBG: a PET reporter probe for imaging herpes simplex virus type 1 thymidine kinase (HSV1-tk) or mutant HSV1-sr39tk's expression. J. Nucl. Med. 47, 706-715 (2006).
22. Yaghoubi, S.S. et al. Imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography. Cancer Gene Ther. 12, 329-339 (2005).
23. Penuelas, I. et al. Positron emission tomography imaging of adenoviral-mediated transgene expression in liver cancer patients. Gastroenterology 128, 1787-1795 (2005).
24. 18F]FHBG. Nucl. Med. Biol. 31 133-138 (2004).
25. 18F]FHBG) for gene therapy. Nucl. Med. Biol. 28, 875-883 (2001).
26. 18F]fluoro-3-hydroxymethylbutyl)guanine for gene therapy studies. Mol. Imaging Biol. 4, 415-424 (2003).
27. Hudson, H.M. & Larkin, R.S. Accelerated image reconstruction using ordered subsets of projection data. IEEE Trans. Med. Imaging 13 601-609 (1994).
28. Loening, A.M. & Gambhir, S.S. AMIDE: A free software tool for multimodality medical image analysis. Mol. Imaging 2, 131-137 (2003).
29. 18F-FHBG for quantitating herpes simplex virus type 1 thymidine kinase reporter gene expression in living animals using PET. J. Nucl. Med. 45, 1560-1570 (2004).
30. Liang, Q. et al. Noninvasive, repetitive, quantitative measurement of gene expression from a bicistronic message by positron emission tomography, following gene transfer with adenovirus. Mol. Ther. 6, 73-82 (2002).
31. Yaghoubi, S.S. et al. Direct correlation between positron emission tomographic images of two reporter genes delivered by two distinct adenoviral vectors. Gene Ther. 8, 1072-1080 (2001).
32. Sundaresan, G. et al. MicroPET imaging of Cre-loxP-mediated conditional activation of a herpes simplex virus type 1 thymidine kinase reporter gene. Gene Ther. 11, 609-618 (2004).
33. De, A., Lewis, X.Z. & Gambhir, S.S. Noninvasive imaging of lentiviral-mediated reporter gene expression in living mice. Mol. Ther. 7, 681-691 (2003).
34. Ray, P., De, A., Min, J.J., Tsien, R.Y. & Gambhir, S.S. Imaging tri-fusion multimodality reporter gene expression in living subjects. Cancer Res. 64, 1323-1330 (2004).
35. Pantuck, A.J. et al. Optimizing prostate cancer suicide gene therapy using herpes simplex virus thymidine kinase active site variants. Hum. Gene Ther. 13, 777-789 (2002).
36. Pantuck, A.J. et al. CL1-SR39: A noninvasive molecular imaging model of prostate cancer suicide gene therapy using positron emission tomography. J. Urol. 168, 1193-1198 (2002).
37. Sun, X. et al. Quantitative imaging of gene induction in living animals. Gene Ther. 8, 1572-1579 (2001).
38. Yu, Y. et al. Quantification of target gene expression by imaging reporter gene expression in living animals. Nat. Med. 6, 933-937 (2000).
39. Yang, H., Berger, F., Tran, C., Gambhir, S.S. & Sawyers, C.L. MicroPET imaging of prostate cancer in LNCAP-SR39TK-GFP mouse xenografts. Prostate 55, 39-47 (2003).
40. Wang, Y. et al. Noninvasive monitoring of target gene expression by imaging reporter gene expression in living animals using improved bicistronic vectors. J. Nucl. Med. 46, 667-674 (2005).
41. Richard, J.-C., Factor, P., Welch, L.C. & Schuster, D.P. Imaging the spatial distribution of transgene expression in the lungs with positron emission tomography. Gene Ther. 10, 2074-2080 (2003).
42. Wu, J.C., Inubushi, M., Sundaresan, G., Schelbert, H.R. & Gambhir, S.S. Positron emission tomography imaging of cardiac reporter gene expression in living rats. Circulation 106, 180-183 (2002).
43. Sen, L. et al. Noninvasive imaging of ex vivo intracoronarily delivered nonviral therapeutic transgene expression in heart. Mol. Ther. 12, 49-57 (2005).
44. Kim, Y.J., Dubey, P., Ray, P., Gambhir, S.S. & Witte, O.N. Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene. Mol. Imaging Biol. 6, 331-340 (2004).
45. Dubey, P. et al. Quantitative imaging of T cell antitumor response by positron-emission tomography. Proc. Natl. Acad. Sci. USA 100 1232-1237 (2003).
46. Cao, F. et al. In vivo visualization of embryonic stem cell survival, proliferation, and migration after cardiac delivery. Circulation 113, 1005-1014 (2006).
47. Penuelas, I., Haberkorn, U., Yaghoubi, S. & Gambhir, S.S. Gene therapy imaging in patients for oncological applications. Eur. J. Nucl. Med. Mol. Imaging 32, S384-S403 (2005)