Translational fusion of two β-subunits of human chorionic gonadotropin results in production of a novel antagonist of the hormone

Endocrinology

Volumn 148, Issue 8, 2007, Pages 3977-3986

  Roy, Satarupa ^a   Setlur, Sunita ^a   Gadkari, Rupali A ^a   Krishnamurthy, H N ^a   Dighe, Rajan R ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN ALPHA BETA SUBUNIT; CHORIONIC GONADOTROPIN BETA BETA SUBUNIT; EPITOPE; FOLLITROPIN; GLYCOPROTEIN; LUTEINIZING HORMONE RECEPTOR; UNCLASSIFIED DRUG;

ALPHA CHAIN; ANALYTIC METHOD; ARTICLE; BETA CHAIN; BINDING AFFINITY; CONTROLLED STUDY; DOSE RESPONSE; HORMONE BINDING; HORMONE DETERMINATION; NONHUMAN; PICHIA; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN INTERACTION;

AMINO ACID SEQUENCE; CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; CLONING, MOLECULAR; DRUG DESIGN; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS;

EID: 34547118332     PISSN: 00137227     EISSN: 00137227     Source Type: Journal    
DOI: 10.1210/en.2006-1499     Document Type: Article

References (51)

1. Pierce JG, Parsons TF 1981 Glycoprotein hormones: structure and function. Annu Rev Biochem 50:465-495
2. Dias JA, Van Roey P 2001 Structural biology of human follitropin and its receptor. Arch Med Res 32:510-519
3. Vassart G, Pardo L, Costagliola S 2004 A molecular dissection of the glycoprotein hormone receptors. Trends Biochem Sci 29:119-126
4. Moyle WR, Lin W, Myers RV, Cao D, Kerrigan JE, Bernard MP 2005 Models of glycoprotein hormone receptor interaction. Endocrine 26:189-205
5. Fan QR, Hendrickson WA 2005 Structure of human follicle-stimulating hormone in complex with its receptor. Nature 433:269-277
6. Fan QR, Hendrickson WA 2005 Structural biology of glycoprotein hormones and their receptors. Endocrine 26:179-188
7. Dias JA 2005 Endocrinology: fertility hormone in repose. Nature 433:203-204
8. Puett D, Li Y, Angelova K, Demars G, Meehan TP, Fanelli F, Narayan P 2005 Structure-function relationships of the luteinizing hormone receptor. Ann NY Acad Sci 1061:41-54
9. Puett D, Bhowmick N, Fernandez LM, Huang J, Wu C, Narayan P 1996 hCG-receptor binding and transmembrane signaling. Mol Cell Endocrinol 125:55-64
10. Narayan P, Wu C, Puett D 2000 Genetic engineering of single-chain gonadotropins and hormone-receptor fusion proteins. Methods 21:59-66
11. Sugahara T, Pixley MR, Minami S, Perlas E, Ben-Menahem D, Hsueh AJ, Boime I 1995 Biosynthesis of a biologically active single peptide chain containing the human common α and chorionic gonadotropin β subunits in tandem. Proc Natl Acad Sci USA 92:2041-2045
12. Fralish GB, Narayan P, Puett D 2003 Consequences of single-chain translation on the structures of two chorionic gonadotropin yoked analogs in α-β and β-α configurations. Mol Endocrinol 17:757-767
13. Sen Gupta C, Dighe RR 2000 Biological activity of single chain chorionic gonadotropin, hCGαβ, is decreased upon deletion of five carboxyl terminal amino acids of the α subunit without affecting its receptor binding. J Mol Endocrinol 24:157-164
14. Dighe RR, Murthy GS, Moudgal NR 1990 Two simple and rapid methods to detect monoclonal antibodies with identical epitope specificities in a large population of monoclonal antibodies. J Immunol Methods 131:229-236
15. Blanchard V, Gadkari RA, Gerwig GJ, Leeflang BR, Dighe RR, Kamerling JP 2007 Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris. Glycoconj J 24:33-47
16. Samaddar M, Catterall JF, Dighe RR 1997 Expression of biologically active β subunit of bovine follicle-stimulating hormone in the methylotrophic yeast Pichia pastoris. Protein Expr Purif 10:345-355
17. Sen Gupta C, Dighe RR 1999 Hyperexpression of biologically active human chorionic gonadotropin using the methylotropic yeast, Pichia pastoris. J Mol Endocrinol 22:273-283
18. Gadkari R, Deshpande R, Dighe RR 2003 Hyperexpression and purification of biologically active human luteinizing hormone and human chorionic gonadotropin using the methylotropic yeast, Pichia pastoris. Protein Expr Purif 32:175-184
19. Dighe RR, Murthy GS, Kurkalli BS, Moudgal NR 1990 Conformation of the α-subunit of glycoprotein hormones: a study using polyclonal and monoclonal antibodies. Mol Cell Endocrinol 72:63-70
20. Dighe RR, Moudgal NR 1983 Use of α- and β-subunit specific antibodies in studying interaction of hCG with Leydig cell receptors. Arch Biochem Biophys 225:490-499
21. Kene PS, Nalavadi VC, Dighe RR, Iyer KS, Mahale SD 2004 Identification of the structural and functional determinants of the extracellular domain of the human follicle stimulating hormone receptor. J Endocrinol 182:501-508
22. Pedersen JB, Lindup WE 1994 Interpretation and analysis of receptor binding experiments which yield non-linear Scatchard plots and binding constants dependent upon receptor concentration. Biochem Pharmacol 47:179-185
23. Gadkari RA, Roy S, Rekha N, Srinivasan N, Dighe RR 2005 Identification of a heterodimer-specific epitope present in human chorionic gonadotrophin (hCG) using a monoclonal antibody that can distinguish between hCG and human LH. J Mol Endocrinol 34:879-887
24. Shenoy AR, Srinivasan N, Subramaniam M, Visweswariah SS 2003 Mutational analysis of the Mycobacterium tuberculosis Rv1625c adenylyl cyclase: residues that confer nucleotide specificity contribute to dimerization. FEBS Lett 545:253-259
25. Shetty J, Marathe GK, Dighe RR 1996 Specific immunoneutralization of FSH leads to apoptotic cell death of the pachytene spermatocytes and spermatogonial cells in the rat. Endocrinology 137:2179-2182
26. Gadkari RA, Sandhya S, Sowdhamini R, Dighe RR 2007 The antigen binding sites of various hCG monoclonal antibodies show homology to different domains of LH receptor. Mol Cell Endocrinol 260-262:23-32
27. Schubert RL, Puett D 2003 Single-chain human chorionic gonadotropin analogs containing the determinant loop of the β-subunit linked to the α-subunit. J Mol Endocrinol 31:157-168
28. Narayan P, Gray J, Puett D 2000 A biologically active single chain human chorionic gonadotropin analog with altered receptor binding properties. Endocrinology 141:67-71
29. Ben-Menahem D, Hyde R, Pixley M, Berger P, Boime I 1999 Synthesis of multisubunit domain gonadotropin complexes: a model for α/β heterodimer formation. Biochemistry 38:15070-15077
30. Kanda M, Jablonka-Shariff A, Sato A, Pixley MR, Bos E, Hiro'oka T, Ben-Menahem D, Boime I 1999 Genetic fusion of an α-subunit gene to the folliclestimulating hormone and chorionic gonadotropin-β subunit genes: production of a bifunctional protein. Mol Endocrinol 13:1873-1881
31. Setlur SR, Dighe RR 2007 Single chain human chorionic gonadotropin, hCGαβ: effects of mutations in the α subunit on structure and bioactivity. Glycoconj J 24:97-106
32. Dighe RR, Muralidhar K, Moudgal NR 1979 Ability of human chorionic gonadotropin β-subunit to inhibit the steroidogenic response to lutropin. Biochem J 180:573-578
33. Moudgal NR, Li CH 1982 β Subunits of human choriogonadotropin and ovine lutropin are biologically active. Proc Natl Acad Sci USA 79:2500-2503
34. Butler SA, Laidler P, Porter JR, Kicman AT, Chard T, Cowan DA, Iles RK 1999 The β-subunit of human chorionic gonadotrophin exists as a homodimer. J Mol Endocrinol 22:185-192
35. Zeng H, Phang T, Song YS, Ji I, Ji TH 2001 The role of the hinge region of the luteinizing hormone receptor in hormone interaction and signal generation. J Biol Chem 276:3451-3458
36. Lobel L, Pollak S, Wang S, Chaney M, Lustbader JW 1999 Expression and characterization of recombinant β-subunit hCG homodimer. Endocrine 10:261-270
37. Moyle WR, Xing Y, Lin W, Cao D, Myers RV, Kerrigan JE, Bernard MP 2004 Model of glycoprotein hormone receptor ligand binding and signaling. J Biol Chem 279:44442-44459
38. Urizar E, Montanelli L, Loy T, Bonomi M, Swillens S, Gales C, Bouvier M, Smits G, Vassart G, Costagliola S 2005 Glycoprotein hormone receptors: link between receptor homodimerization and negative cooperativity. EMBO J 24:1954-1964
39. Moyle WR, Ehrlich PH, Canfield RE 1982 Use of monoclonal antibodies to subunits of human chorionic gonadotropin to examine the orientation of the hormone in its complex with receptor. Proc Natl Acad Sci USA 79:2245-2249
40. Milius RP, Midgley Jr AR, Birken S 1983 Preferential masking by the receptor of immunoreactive sites on the α subunit of human choriogonadotropin. Proc Natl Acad Sci USA 80:7375-7379
41. Remy JJ, Couture L, Pantel J, Haertle T, Rabesona H, Bozon V, Pajot-Augy E, Robert P, Troalen F, Salesse R, Bidart JM 1996 Mapping of HCG-receptor complexes. Mol Cell Endocrinol 125:79-91
42. Sohn J, Ryu K, Sievert G, Jeoung M, Ji I, Ji TH 2002 Follicle-stimulating hormone interacts with exoloop 3 of the receptor. J Biol Chem 277:50165-50175
43. Couture L, Remy JJ, Rabesona H, Troalen F, Pajot-Augy E, Bozon V, Haertle T, Bidart JM, Salesse R 1996 A defined epitope on the human choriogonadotropin α-subunit interacts with the second extracellular loop of the transmembrane domain of the lutropin/choriogonadotropin receptor. Eur J Biochem 241:627-632
44. Moyle WR, Campbell RK, Rao SN, Ayad NG, Bernard MP, Han Y, Wang Y 1995 Model of human chorionic gonadotropin and lutropin receptor interaction that explains signal transduction of the glycoprotein hormones. J Biol Chem 270:20020-20031
45. Myers RV, Wang Y, Moyle WR 2000 The surface of α-subunit loop 1 distant from the subunit interface is exposed in the hCG lutropin receptor complex. Biochim Biophys Acta 1475:390-394
46. Gether U 2000 Uncovering molecular mechanisms involved in activation of G protein-coupled receptors. Endocr Rev 21:90-113
47. Ji I, Lee C, Song Y, Conn PM, Ji TH 2002 Cis- and trans-activation of hormone receptors: the LH receptor. Mol Endocrinol 16:1299-1308
48. Szkudlinski MW, Fremont V, Ronin C, Weintraub BD 2002 Thyroid-stimulating hormone and thyroid-stimulating hormone receptor structure-function relationships. Physiol Rev 82:473-502
49. Grossmann M, Weintraub BD, Szkudlinski MW 1997 Novel insights into the molecular mechanisms of human thyrotropin action: structural, physiological, and therapeutic implications for the glycoprotein hormone family. Endocr Rev 18:476-501
50. Hong S, Ji I, Ji TH 1999 The α-subunit of human choriogonadotropin interacts with the exodomain of the luteinizing hormone/choriogonadotropin receptor. Endocrinology 140:2486-2493
51. Hong SH, Ji IH, Ji TH 1999 The β-subunit of human choriogonadotropin interacts with the exodomain of the luteinizing hormone/choriogonadotropin receptor and changes its interaction with the α-subunit. Mol Endocrinol 13:1285-1294