Single-chain human chorionic gonadotropin analogs containing the determinant loop of the β-subunit linked to the α-subunit

Journal of Molecular Endocrinology

Volumn 31, Issue 1, 2003, Pages 157-168

  Schubert, R L ^a   Puett, D ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN ALPHA SUBUNIT; CHORIONIC GONADOTROPIN BETA SUBUNIT; COMPLEMENTARY DNA; CYCLIC AMP; G PROTEIN COUPLED RECEPTOR; HYBRID PROTEIN; LUTEINIZING HORMONE RECEPTOR;

ALPHA CHAIN; AMINO TERMINAL SEQUENCE; ANTIGEN BINDING; ARTICLE; BETA CHAIN; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYSTAL STRUCTURE; GENE EXPRESSION; GENETIC TRANSFECTION; HORMONE BINDING; HORMONE RECEPTOR INTERACTION; HORMONE STRUCTURE; HUMAN; HUMAN CELL; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN ENGINEERING;

BINDING SITES; CELL LINE; CHORIONIC GONADOTROPIN; CRYSTALLOGRAPHY, X-RAY; CYCLIC AMP; HUMANS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN SUBUNITS; RECEPTORS, LH; TRANSFECTION;

EID: 0042353818     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.0.0310157     Document Type: Article

References (42)

1. Ascoli M, Fanelli F & Segaloff DL 2002 The lutropin/choriogonadotropin receptor, a 2002 perspective. Endocrine Reviews 23 141-174.
2. Ben-Menahem D, Kudo M, Pixley MR, Sato A, Suganuma N, Perlas E, Hsueh AJW & Boime I 1997 The biologic action of single-chain choriogonadotropin is not dependent on the individual disulfide bonds of the β subunit. Journal of Biological Chemistry 272 6827-6830.
3. Ben-Menahem D, Jablonka-Shariff A, Hyde RK, Pixley MR, Srivastava S, Berger P & Boime I 2001 The position of the α and β subunits in a single chain variant of human chorionic gonadotropin affects the heterodimeric interaction of the subunits and receptor-binding epitopes. Journal of Biological Chemistry 276 29871-29879.
4. Bielinski M & Boime I 1992 Site-directed mutagenesis defines a domain in the gonadotropin α subunit required for assembly with the chorionic gonadotropin β-subunit. Molecular Endocrinology 6 267-271.
5. Campbell RK, Dean-Emig DM & Moyle WR 1991 Conversion of human choriogonadotropin into a follitropin by protein engineering. PNAS 88 760-764.
6. Chen W & Bahl OP 1991 Recombinant carbohydrate variant of the human choriogonadotropin β-subunit (hCGβ) descarboxyl terminus (115-145). Journal of Biological Chemistry 266 6246-6251.
7. Chen F & Puett D 1991a Delineation via site-directed mutagenesis of the carboxyl-terminal region of human choriogonadotropin β required for subunit assembly and biological activity. Journal of Biological Chemistry 266 6904-6908.
8. Chen F & Puett D 1991b Contributions of arginines -43 and -94 of human choriogonadotropin β to receptor binding and activation determined by oligonucleotide-based mutagenesis. Biochemistry 30 10171-10175.
9. Chen F, Wang Y & Puett D 1991 Role of the invariant aspartic acid 99 of human choriogonadotropin β in receptor binding and biological activity Journal of Biological Chemistry 266 19357-19361.
10. Chen F, Wang Y & Puett D 1992 The carboxy-terminal region of the glycoprotein hormone α-subunit: contributions to receptor binding and signaling in human chorionic gonadotropin. Molecular Endocrinology 6 914-919.
11. Dias JA, Zhang Y & Liu X 1994 Receptor binding and functional properties of chimeric human follitropin prepared by an exchange between a small hydrophilic intercystine loop of human follitropin and human lutropin. Journal of Biological Chemistry 269 25289-25294.
12. Dufau ML 1998 The luteinizing hormone receptor. Annual Review of Biochemistry 60 461-496.
13. El-Deiry S, Kaetzel D, Kennedy G, Nilson J & Puett D 1989 Site-directed mutagenesis of the human chorionic gonadotropin β subunit: bioactivity of a heterologous hormone, bovine α-human des-(122 145)β. Molecular Endocrinology 3 1523-1528.
14. Fox KM, Dias JA & Van Roey P 2001 Three-dimensional structure of human follicle-stimulating hormone. Molecular Endocrinology 15 378-389.
15. Fralish GB, Narayan P & Puett D 2001 High-level expression of a functional single-chain human chorionic gonadotropin luteinizing hormone receptor ectodomain complex in insect cells. Endocrinology 142 1517-1524.
16. Grossmann M, Szkudlinski MW, Wong R, Dias JA, Ji TH & Weintraub BD 1997 Substitution of the seat-belt region of the thyroid-stimulating hormone (TSH) β-subunit with the corresponding regions of choriogonadotropin or follitropin confers luteotropic but not follitropic activity to chimeric TSH. Journal of Biological Chemistry 272 15532-15540.
17. Han Y, Bernard MP & Moyle WR 1996 hCGβ residues 94-96 alter LH activity without appearing to make receptor contacts. Molecular and Cellular Endocrinology 124 151-161 [erratum: 1997 129 237-239].
18. Hearn MTW & Gomme PT 2000 Molecular architecture and biorecognition process of the cysteine knot protein superfamily: part 1. The glycoprotein hormones. Journal of Molecular Recognition 13 223-278.
19. Heikoop JC, Van Beuningin-De VMM, Van Den Boogaart P & Grootenhuis PDJ 1997 Evaluation of subunit truncation and the nature of the spacer for single chain human gonadotropins. European Journal of Biochemistry 245 656-662.
20. Huang J, Chen F & Puett D 1993a Amino/carboxyl-terminal deletion mutants of human choriogonadotropin. Journal of Biological Chemistry 268 9311-9315.
21. Huang J, Ujihara M, Xia H, Chen F, Yoshida H & Puett D 1993b Mutagenesis of the 'determinant loop' region of human choriogonadotropin β. Molecular and Cellular Endocrinology 90 211-218.
22. Keutmann HT 1992 Receptor-binding regions in human glycoprotein hormones. Molecular and Cellular Endocrinology 86 C1-C6.
23. Keutmann HT, Mason KA, Kitzmann K & Ryan RJ 1989 Role of the β93 100 determinant loop sequence in receptor binding and biological activity of human luteinizing hormone and chorionic gonadotropin. Molecular Endocrinology 3 526-531.
24. Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Morgan FJ & Isaacs NW 1994 Crystal structure of human chorionic gonadotropin. Nature 369 455-461.
25. Liu C, Roth KE, Shepard BA, Shaller JB & Dias JA 1993 Site-directed alanine mutagenesis of Phe33, Arg35, and Arg42-Ser43-Lys44 in the human gonadotropin alpha-subunit. Journal of Biological Chemistry 268 21613-21617.
26. Matzuk MM, Hsueh AJW, Lapolt P, Tsafriri A, Keene JL & Boime I 1990 The biological role of the carboxyl-terminal extension of human chorionic gonadotropin β-subunit. Endocrinology 126 376-383.
27. Moore WT, Burleigh BD & Ward DN 1980 Chorionic gonadotropins: comparative studies and comments on relationships to other glycoprotein hormones. In Chorionic Gonadotropins, pp 89-126. Ed. SJ Segal. New York: Plenum Press.
28. Narayan P, Wu C & Puett D 1995 Functional expression of yoked human chorionic gonadotropin in baculovirus-infected insect cells. Molecular Endocrinology 9 1720-1726.
29. Narayan P, Gray J & Puett D 2000a A biologically active single chain human chorionic gonadotropin analog with altered receptor binding properties. Endocrinology 141 67-71.
30. Narayan P, Wu C & Puett D 2000b Genetic engineering of single chain gonadotropins and hormone-receptor fusion proteins. Methods 21 59-66.
31. Narayan P, Gray J & Puett D 2002 Yoked complexes of human choriogonadotropin and the lutropin receptor: evidence that monomeric individual subunits are inactive. Molecular Endocrinology 16 2733-2735.
32. Puett D & Narayan P 2000 Designer gonadotropins and receptors: the prospect of recombinant technology. In Ovulation: Evolving Scientific and Clinical Concepts, pp 295-307. Ed. EY Adashi. New York: Springer-Verlag.
33. Puett D & Narayan P 2002 Fusion proteins of human gonadotropins and gonadotropin receptor complexes. Indian Journal of Experimental Biology 40 115-423.
34. Puett D, Huang J & Xia H 1994 Delineation of subunit and receptor contact sites by site-directed mutagenesis of hCGβ. In Glycoprotein Hormones, pp 122-134. Eds JW Lustbader, D Puett & RW Ruddon. New York: Springer-Verlag.
35. Salesse R, Bidart JM, Troalen F & Garnier J 1990 Peptide mapping of intersubunit and receptor interactions of human choriogonadotropin. Molecular and Cellular Endocrinology 68 113-119.
36. Schubert RL, Narayan P & Puett D 2003 Specificity of cognate ligand-receptor interaction: fusion proteins of hGG and the heptahelical receptors for human luteinizing hormone, thyroid-stimulating hormone, and follicle-stimulating hormone. Endocrinology 144 129-137.
37. Sen Gupta C & Dighe RR 2000 Biological activity of single chain choriogonadotropin, hCGαβ is decreased upon deletion of five carboxyl terminal amino acids of the α subunit without affecting its receptor binding. Journal of Molecular Endocrinology 24 157-164.
38. Slaughter S, Wang Y, Myers RV & Moyle WR 1995 The lutropin β-subunit N-terminus facilitates subunit combination by offsetting the inhibitory effects of residues needed for LH activity. Molecular and Cellular Endocrinology 112 21-25.
39. Sugahara T, Pixley MR, Minami S, Perlas E, Ben-Menahem D, Hsueh AJ & Boime I 1995 Biosynthesis of a biologically active single peptide chain containing the human common alpha and chorionic gonadotropin beta subunits in tandem. PNAS 92 2041-2045.
40. Wu H, Lustbader JW, Liu Y, Canfield RE & Hendrickson WA 1994 Structure of human chorionic gonadotropin at 2.6 Å resolution from MAD analysis of the selenomethionyl protein. Structure 2 545-558.
41. Wu C, Narayan P & Puett D 1996 protein engineering of a novel constitutively active hormone-receptor complex. Journal of Biological Chemistry 271 31638-31642.
42. Yoo J, Zeng H, Ji I, Murdoch WJ & Ji TH 1993 COOH-terminal amino acids of the alpha subunit play common and different roles in human choriogonadotropin and follitropin. Journal of Biological Chemistry 268 13034-13042.