Identification of a heterodimer-specific epitope present in human chorionic gonadotrophin (hCG) using a monoclonal antibody that can distinguish between hCG and human LH

Journal of Molecular Endocrinology

Volumn 34, Issue 3, 2005, Pages 879-887

  Gadkari, R A ^a   Roy, S ^a   Rekha, N ^a   Srinivasan, N ^a   Dighe, R R ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN BETA SUBUNIT; CONTRACEPTIVE VACCINE; IMMUNOGLOBULIN G; LUTEINIZING HORMONE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY E12; NEUTRALIZING ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ACTIVE IMMUNIZATION; ANTIBODY SPECIFICITY; ANTIGEN RECOGNITION; ARTICLE; CORPUS LUTEUM; EPITOPE MAPPING; HUMAN; IMMUNOCONTRACEPTION; MENSTRUAL CYCLE; NONHUMAN; PASSIVE IMMUNIZATION; PICHIA PASTORIS; PREGNANCY TERMINATION; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; CHORIONIC GONADOTROPIN; DIMERIZATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; EPITOPES; HUMANS; LUTEINIZING HORMONE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RADIOIMMUNOASSAY; RECEPTORS, LH; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 21344458801     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.1.01683     Document Type: Article

References (33)

1. Berger P, Sturgeon G, Bidart JM, Paus E, Gerth R, Niang M, Bristow A, Birken S & Stenman UH 2002 The ISOBM TD-7 Workshop on hCG and related molecules. Towards user-oriented standardization of pregnancy and tumor diagnosis: assignment of epitopes to the three-dimensional structure of diagnostically and commercially relevant monoclonal antibodies directed against human chorionic gonadotropin and derivatives. Tumour Biology 23 1-38.
2. Blanchard V, Gadkari RA, Gerrit J. Gerwig GJ, Bas R. Leeflang, Dighe RR & Kamerling JP 2003 Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast, Pichia pastoris. 12th European Carbohydrate Symposium, 6-11 July, Grenoble, France.
3. Charrel-Dennis M, Jackson AM, Lund T, Lapthorn AJ, Berger P, Roitt IM & Delves PJ 2004 The major hormone-specific discontinuous epitopes on human chorionic gonadotrophin. Journal of Molecular Endocrinology 32 571-581.
4. Delves PJ 2004 How far from a hormone-based contraceptive vaccine? Journal of Reproductive Immunology 62 69-78.
5. Dighe RR & Moudgal NR 1983 Use of alpha- and beta-subunit specific antibodies in studying interaction of hCG with Leydig cell receptors. Archives of Biochemistry and Biophysics 225 490-499.
6. Dighe RR, Murthy GS & Moudgal NR 1990a Two simple and rapid methods to detect monoclonal antibodies with identical epitope specificities in a large population of monoclonal antibodies. Journal of Immunological Methods 131 229-236.
7. Dighe RR, Murthy GS, Kurkalli BS & Moudgal NR 1990b Conformation of the alpha-subunit of glycoprotein hormones: a study using polyclonal and monoclonal antibodies. Molecular and Cell Endocrinology 72 63-70.
8. Dirnhofer S & Berger P 1995 Vaccination for birth control. International Archives of Allergy and Immunology 108 350-354.
9. Dirnhofer S, Wick G & Berger P 1994 The suitability of human chorionic gonadotropin (hCG)-based birth control vaccines. Immunology Today 15 455-501.
10. Evans S 1993 SETOR: hardware lighted three-dimensional solid model representations of macromolecules. Journal of Molecular Graphics 11 134-138.
11. Fox HKF 1970 Immunofluorescent localisation of chorionic gonadotrophin in the placenta and in tissue cultures of human trophoblast. Journal of Pathology 101 277-282.
12. Gadkari R, Deshpande R & Dighe RR 2003 Hyperexpression and purification of biologically active human luteinizing hormone and human chorionic gonadotropin using the methylotropic yeast, Pichia pastoris. Protein Expression and Purification 32 175-184.
13. Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber E & Crea R 1988 Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analog produced in Escherichia coli. PNAS 85 5879-5883.
14. Jackson AM, Klonisch T, Lapthorn AJ, Berger P, Isaacs NW, Delves PJ, Lund T & Roitt IM 1996 Identification and selective destruction of shared epitopes in human chorionic gonadotropin beta subunit. Journal of Reproductive Immunology 31 21-36.
15. Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Morgan FJ & Isaacs NW 1994 Crystal structure of human chorionic gonadotropin. Nature 369 455-461.
16. Lee B & Richards FM 1971 The interpretation of protein structures: estimation of static accessibility. Journal of Molecular Biology 55 379-400.
17. Louvet JP, Ross GT, Birken S & Canfield RE 1974 Absence of neutralizing effect of antisera to the unique structural region of human chorionic gonadotropin. Journal of Clinical Endocrinology and Metabolism 39 1155-1158.
18. Martinez HM 1988 A flexible multiple sequence alignment program. Nucleic Acids Research 16 1683-1691.
19. Mitchison N 1990 Gonadotropic vaccines. Current Opinion in Immunology 2 725-727.
20. Mizuguchi K, Deane CM, Blundell TL, Johnson MS, Overington JP 1998 JOY: protein sequence-structure representation and analysis. Bioinformatics 14 617-623.
21. Moyle WR, Campbell RK, Rao SN, Ayad NG, Bernard MP, Han Y & Wang Y 1995 Model of human chorionic gonadotropin and lutropin receptor interaction that explains signal transduction of the glycoprotein hormones. Journal of Biological Chemistry 270 20020-20031.
22. Nicholls A, Sharpe KA & Honig B 1991 Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins 11 281-296.
23. Porakishvili N, Chiesa MD, Chikadze N, Martensen P, Justesen J, Lund T, Delves PJ & Roitt IM 2002 Elimination of luteinizing hormone cross-reactive epitopes from human chorionic gonadotropin. Vaccine 20 2053-2059.
24. Schwarz S, Berger P, Nelboeck E, Khashabi D, Panmoung W, Klieber R & Wick G 1988 Probing the receptor-interaction of glycoprotein hormones with monoclonal antibodies. Journal of Receptor Research 8 437-453.
25. Sen Gupta C & Dighe RR 1999 Hyperexpression of biologically active human chorionic gonadotropin using the methylotropic yeast, Pichia pastons. Journal of Molecular Endocrinology 22 273-283.
26. Sen Gupta C & Dighe RR 2000 Biological activity of single chain chorionic gonadotropin, hGGalphabeta, is decreased upon deletion of five carboxyl terminal amino acids of the alpha subunit without affecting its receptor binding. Journal of Molecular Endocrinology 24 157-164.
27. Stevens VC 1996 Progress in the development of human chorionic gonadotropin antifertility vaccines. American Journal of Reproductive Immunology 35 148-155.
28. Strauss JF III, Gafvels M & King BF 1995 Placental Hormones, edn 3, pp. Eds LJD et al. Philadelphia, PA: WB Saunders.
29. Sunita BR 2003 Single Chain Glycoprotein Hormones: Structure-Function Studies. PhD thesis. Bangalore India. Indian Institute of Science.
30. Talwar GP, Singh O, Pal R, Chatterjee N, Sahai P, Dhall K, Kaur J, Das SK, Suri S, Buckshee K et al. 1994 A vaccine that prevents pregnancy in women. PNAS 91 8532-8536.
31. Venkatesh N & Murthy GS 1997 Immunochemical approach to the mapping of an assembled epitope of human chorionic gonadotropin: proximity of CTP-alpha to the receptor binding region of the beta-subunit. Journal of Immunological Methods 202 173-182.
32. Venkatesh N, Krishnaswamy S, Meuris S & Murthy GS 1999 Epitope analysis and molecular modeling reveal the topography of the C-terminal peptide of the beta-subunit of human chorionic gonadotropin. European Journal of Biochemistry 265 1061-1066.
33. Wu H, Lustbader JW, Liu Y, Canfield RE & Hendrickson WA 1994 Structure of human chorionic gonado tropin at 2.6 Å resolution from MAD analysis of the selenomethionyl protein. Structure 2 545-558.