Multivariate-activity mining for molecular quasi-species in a glutathione transferase mutant library

Protein Engineering, Design and Selection

Volumn 20, Issue 5, 2007, Pages 243-256

  Kurtovic, Sanela ^a   Runarsdottir, Arna ^a   Emrén, Lars O ^a   Larsson, Anna Karin ^a   Mannervik, Bengt ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Directed evolution; DNA shuffling; Glutathione transferase; Library; Multivariate analysis

Indexed keywords

DNA; GENE EXPRESSION; MOLECULAR BIOLOGY; MUTAGENESIS; PRINCIPAL COMPONENT ANALYSIS; SUBSTRATES;

DIRECTED EVOLUTION; DNA SHUFFLING; GLUTATHIONE TRANSFERASE; MULTIVARIATE ANALYSIS;

ENZYME ACTIVITY;

DNA; ENZYME VARIANT; GLUTATHIONE TRANSFERASE; RECOMBINANT PROTEIN;

ARTICLE; DNA SHUFFLING; ENZYME ACTIVITY; GENE CLUSTER; GENE LIBRARY; GENETIC SCREENING; MULTIVARIATE ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; AMINO ACID SUBSTITUTION; CATALYSIS; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SEQUENCE ANALYSIS;

AMINO ACID SUBSTITUTION; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; GENE LIBRARY; GLUTATHIONE TRANSFERASE; HUMANS; MULTIVARIATE ANALYSIS; MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SUBSTRATE SPECIFICITY;

EID: 34447520873     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm017     Document Type: Article

References (39)

1. Aharoni,A., Griffiths,A.D. and Tawfik,D.S. (2005) Curr. Opin. Chem. Biol., 9, 210-216.
2. Anantharaman,V., Aravind,L. and Koonin,E.V. (2003) Curr. Opin. Chem. Biol., 7, 12-20.
3. Aravind,L., Iyer,L.M. and Koonin,E.V. (2006) Curr. Opin. Struct. Biol., 16, 409-419.
4. Armstrong,R.N. (1997) Chem. Res. Toxicol., 10, 2-18.
5. Bloom,J.D., Meyer,M.M., Meinhold,P., Otey,C.R., MacMillan,D. and Arnold,F.H. (2005) Curr. Opin. Struct. Biol., 15, 447-452.
6. Choi,I.G. and Kim,S.H. (2006) Proc. Natl Acad. Sci. USA, 103, 14056-14061.
7. Comstock,K.E., Widersten,M., Hao,X.-Y., Henner,W.D. and Mannervik,B. (1994) Arch. Biochem. Biophys., 311, 487-495.
8. Eigen,M., McCaskill,J. and Schuster,P. (1988) J. Phys. Chem., 92, 6881-6891.
9. Eklund,B.I., Edalat,M., Stenberg,G. and Mannervik,B. (2002) Anal. Biochem., 309, 102-108.
10. Emrén,L.O., Kurtovic,S., Runarsdottir,A., Larsson,A-K. and Mannervik,B. (2006) Proc. Natl Acad. Sci. USA, 103, 10866-10870.
11. Farinas,E.T., Bulter,T. and Arnold,F.H. (2001) Curr. Opin. Biotechnol., 12, 545-551.
12. Habig,W.H., Pabst,M.J. and Jakoby,W.B. (1974) J. Biol. Chem., 249, 7130-7139.
13. Halgren,T.A. (1999) J. Comput. Chem., 20, 730-748.
14. Hansson,L.O., Bolton-Grob,R., Massoud,T. and Mannervik,B. (1999a) J. Mol. Biol., 287, 265-276.
15. Hansson,L.O., Bolton-Grob,R., Widersten,M. and Mannervik,B. (1999b) Protein Sci., 8, 2742-2750.
16. Hao,X.-Y., Castro,V.M., Bergh,J., Sundström,B. and Mannervik,B. (1994) Biochim. Biophys. Acta, 1225, 223-230.
17. Ivarsson,Y. and Mannervik,B. (2005) Protein Eng. Des. Sel., 18, 607-616.
18. Ivarsson,Y., Mackey,A.J., Edalat,M., Pearson,W.R. and Mannervik,B. (2003) J. Biol. Chem., 278, 8733-8738.
19. Jensen,D.E. and Stelman,G.J. (1987) Carcinogenesis, 8, 1791-1800.
20. Johansson,A-S., Bolton-Grob,R. and Mannervik,B. (1999) Protein Expr. Purif., 17, 105-112.
21. Josephy,P.D. and Mannervik,B. (2006) Molecular Toxicology. Oxford University Press, Inc., New York.
22. Keen,J.H. and Jakoby,W.B. (1978) J. Biol. Chem., 253, 5654-5657.
23. Kolm,R.H., Danielson,U.H., Zhang,Y.S., Talalay,P. and Mannervik,B. (1995) Biochem. J., 311, 453-459.
24. Koonin,E.V., Mushegian,A.R., Tatusov,R.L., Altschul,S.F., Bryant,S.H., Bork,P. and Valencia,A. (1994) Protein Sci, 3, 2045-2054.
25. Krzanowski,W.J. (2000) Principles of Multivariate Analysis - A Users's Perspective. Oxford University Press, Inc., New York.
26. Larsson,A-K., Emrén,L.O., Bardsley,W.G. and Mannervik,B. (2004) Protein Eng. Des. Sel., 17, 49-55.
27. Mannervik,B. (2005) Methods Enzymol., 401, 254-265.
28. Meyer,M.M., Hochrein,L. and Arnold,F.H. (2006) Protein Eng. Des. Sel., 19, 563-570.
29. Ness,J.E., Welch,M., Giver,L., Bueno,M., Cherry,J.R., Borchert,T.V., Stemmer,W.P. and Minshull,J. (1999) Nat. Biotechnol., 17, 893-896.
30. Ness,J.E., Del Cardayre,S.B., Minshull,J. and Stemmer,W.P. (2000) Adv. Protein Chem., 55, 261-292.
31. Norrgård,M.A., Ivarsson,Y., Tars,K. and Mannervik,B. (2006) Proc. Natl. Acad. Sci. USA, 103, 4876-4881.
32. Orengo,C.A. and Thornton,J.M. (2005) Annu. Rev. Biochem., 74, 867-900.
33. Otey,C.R., Silberg,J.J., Voigt,C.A., Endelman,J.B., Bandara,G. and Arnold,F.H. (2004) Chem. Biol., 11, 309-318.
34. Ricci,G., Caccuri,A.M., Lo Bello,M., Pastore,A., Piemonte,F. and Federici,G. (1994) Anal. Biochem., 218, 463-465.
35. Shokeer,A., Larsson,A-K. and Mannervik,B. (2005) Biochem. J., 388, 387-392.
36. Svendsen,A. (ed.) (2004) Enzyme Functionality: Design, Engineering, and Screening. Marcel Dekker Inc., New York.
37. Taly,V., Urban,P., Truan,G. and Pompon,D. (2007) Biochim. Biophys. Acta, 1770, 446-457.
38. Valetti,F. and Gilardi,G. (2004) Nat. Prod. Rep., 21, 490-511.
39. White,R.H. (2006) J. Bacteriol., 188, 3431-3432.