Optimizing the heterologous expression of glutathione transferase

Methods in Enzymology

Volumn 401, Issue , 2005, Pages 254-265

  Mannervik, Bengt ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; RECOMBINANT PROTEIN;

ARTICLE; CLONE; CODON; DNA LIBRARY; ESCHERICHIA COLI; HUMAN; MUTATION; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ESCHERICHIA COLI;

EID: 30144432505     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)01016-5     Document Type: Review

References (37)

1. Ålin P., Mannervik B., and Jörnvall H. Cytosolic rat liver glutathione transferase 4-4. Primary structure of the protein reveals extensive differences between homologous glutathione transferases of classes Alpha and Mu Eur. J. Biochem. 156 1986 343 350
2. Aronsson A.-C., and Mannervik B. Characterization of glyoxalase I purified from pig erythrocytes by affinity chromatography Biochem. J. 165 1977 503 509
3. Baez S., Segura-Aguilar J., Widersten M., Johansson A.-S., and Mannervik B. Glutathione transferases catalyse the detoxication of oxidized metabolites (o-quinones) of catecholamines and may serve as an antioxidant system preventing degenerative cellular processes Biochem. J. 324 1997 25 28
4. Bass S., and Yang M. Expressing cloned genes in Escherichia coli T.E. Creighton "Protein Function. A Practical Approach" 1998 IRL Press Oxford 29 55
5. Björnestedt R., Widersten M., Board P.G., and Mannervik B. Design of two chimaeric human-rat class Alpha glutathione transferases for probing the contribution of C-terminal segments of protein structure to the catalytic properties Biochem. J. 282 1992 505 510
6. Bücheler U.S., Werner D., and Schirmer R.H. Random silent mutagenesis in the initial triplets of the coding region: A technique for adapting human glutathione reductase-encoding cDNA to expression in Escherichia coli Gene 96 1990 271 276
7. Bulmer M. Are codon usage patterns in unicellular organisms determined by selection-mutation balance? J. Evol. Biol. 1 1988 15 26
8. Chaga G., Widersten M., Andersson L., Porath J., Danielson U.H., and Mannervik B. Engineering of a metal coordinating site into human glutathione transferase M1-1 based on immobilized metal ion affinity chromatography of homologous rat enzymes Protein Engineer 7 1994 1115 1119
9. Chambers I., Frampton J., Goldfarb P., Affara N., McBain W., and Harrison P.R. The structure of the mouse glutathione peroxidase gene: The selenocysteine in the active site is encoded by the 'termination' codon, TGA EMBO J. 5 1986 1221 1227
10. Gustafsson A., and Mannervik B. Benzoic acid derivatives induce recovery of catalytic activity in the partially inactive Met208Lys mutant of human glutathione transferase A1-1 J. Mol. Biol. 288 1999 787 800
11. Habig W.H., Pabst M.J., and Jakoby W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation J. Biol. Chem. 249 1974 7130 7139
12. Jemth P., Stenberg G., Chaga G., and Mannervik B. Heterologous expression, purification and characterization of rat class Theta glutathione transferase T2-2 Biochem. J. 316 1996 131 136
13. Jiang F., and Mannervik B. Optimized heterologous expression of glutathione reductase from cyanobacterium Anabaena PCC 7120 and characterization of the recombinant protein Protein Expression Purif. 15 1999 92 98
14. Johansson A.-S., Bolton-Grob R., and Mannervik B. Use of silent mutations in cDNA encoding human glutathione transferase M2-2 for optimized expression in Escherichia coli Protein Expression Purif. 17 1999 105 112
15. Kane J.F. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli Curr. Opin. Biotechnol. 6 1995 494 500
16. Kolm R.H., Stenberg G., Widersten M., and Mannervik B. High-level bacterial expression of human glutathione transferase P1-1 encoded by semisynthetic DNA Protein Expression Purif. 6 1995 265 271
17. Mannervik B., and Guthenberg C. Glutathione transferase (human placenta) Meth. Enzymol. 77 1981 231 235
18. Makrides S.C. Strategies for achieving high-level expression of genes in Escherichia coli Microbiol. Rev. 60 1996 512 538
19. Nilsson L.O., and Mannervik B. Improved heterologous expression of human glutathione transferase A4-4 by random silent mutagenesis of codons in the 5′-region Biochim. Biophys. Acta 1528 2001 101 106
20. Poole E.S., Brown C.M., and Tate W.P. The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli EMBO J. 14 1995 151 158
21. Porath J., Carlsson J., Olsson I., and Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation Nature 258 1975 598 599
22. Ridderström M., and Mannervik B. Optimized heterologous expression of the human zinc enzyme glyoxalase I Biochem. J. 314 1996 463 467
23. Ridderström M., Saccucci F., Hellman U., Bergman T., Principato G., and Mannervik B. Molecular cloning, heterologous expression and characterization of human glyoxalase II J. Biol. Chem. 271 1996 319 323
24. Rowe J.D, Nieves E., and Listowsky I. Subunit diversity and tissue distribution of human glutathione S-transferases: Interpretations based on electrospray ionization-MS and peptide sequence-specific antisera Biochem. J. 325 1997 481 486
25. Sharp P.M., and Bulmer M. Selective differences among translation termination codons Gene 63 1988 141 145
26. Simons P.C., and Vander Jagt D.L. Purification of glutathione S-transferases by glutathione affinity chromatography Methods Enzymol. 77 1981 235 237
27. Smith D.B., and Johnson K.S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 1988 31 40
28. Sørensen H.P., and Mortensen K.K. Advanced genetic strategies for recombinant protein expression in Escherichia coli J. Biotechnol. 115 2005 113 128
29. Stanley K.K. Solubilization and immune-detection of β-galactosidase hybrid proteins carrying foreign antigenic determinants Nucleic Acids Res. 11 1983 4077 4092
30. Tan K.L., and Board P.G. Purification and characterization of a recombinant human Theta-class glutathione transferase (GSTT2-2) Biochem. J. 315 1996 727 732
31. Towbin H., Staehelín T., and Gordon T. Electrophoretic transfer of proteins from polyacrylamide gels to mitocellulose sheets: Procedure and some applications Proc. Natl. Acad. Sci. USA 76 1979 4350 4354
32. Voges D., Watzele M., Nemetz C., Wizemann S., and Buchberger B. Analyzing and enhancing mRNA translation efficiency in an Escherichia coli in vitro expression system Biochem. Biophys. Res. Commun. 318 2004 601 614
33. Vorachek W.R., Pearson W.R., and Rule G.S. Cloning, expression, and characterization of a class-Mu glutathione transferase from human muscle, the product of the GST4 locus Proc. Natl. Acad. Sci. USA 88 1991 4443 4447
34. Widersten M., Huang M., and Mannervik B. Optimized heterologous expression of the polymorphic human glutathione transferase M1-1 based on silent mutations in the corresponding cDNA Protein Expression Purif. 7 1996 367 372
35. Wisén S., Jiang F., Bergman B., and Mannervik B. Expression and purification of the transcription factor NtcA from the cyanobacterium Anabaena PCC 7120 Protein Expression Purif. 17 1999 351 357
36. Wu X., and Jörnvall H. Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance Biochem. Biophys. Res. Commun. 313 2004 89 96
37. Yilmaz S., Widersten M., Emahazion T., and Mannervik B. Generation of a Ni(II) binding site by introduction of a histidine cluster in the structure of human glutathione transferase A1-1 Prot. Engineer 8 1995 1163 1169