Sugar-mediated lyoprotection of purified human CYP3A4 and CYP2D6

Journal of Biotechnology

Volumn 130, Issue 4, 2007, Pages 436-440

  Chefson, Amandine ^a   Zhao, Jin ^a   Auclair, Karine ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

Biocatalyst; Cryoprotection; Freeze drying; Lyophilization; P450; Saccharide

Indexed keywords

DRUG PRODUCTS; ENZYME ACTIVITY; HYDRATION; METABOLISM; SUGAR (SUCROSE);

COLYOPHILIZATION; CRYOPROTECTION; FREEZE-DRYING; LYOPHILIZATION;

BIOCATALYSTS;

CYCLODEXTRIN; CYTOCHROME P450 2D6; CYTOCHROME P450 3A4; MANNITOL; SUCROSE; TREHALOSE;

ARTICLE; BIOCATALYST; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; FREEZE DRYING; HUMAN; PRIORITY JOURNAL;

CARBOHYDRATES; CATALYSIS; CYTOCHROME P-450 CYP2D6; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME ACTIVATION; HUMANS;

EID: 34347385387     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.05.009     Document Type: Article

References (36)

1. Allison S.D., Chang B., Randolph T.W., and Carpenter J.F. Hydrogen bonding between sugar and protein is responsible for inhibition of dehydration-induced protein unfolding. Arch. Biochem. Biophys. 365 (1999) 238-298
2. Arakawa T., Prestrelski S.J., Kenney W.C., and Carpenter J.F. Factors affecting short-term and long-term stabilities of proteins. Adv. Drug Deliv. Rev. 46 (2001) 307-326
3. Bernhardt R. Cytochromes P450 as versatile biocatalysts. J. Biotechnol. 124 (2006) 128-145
4. Broos J., Verboom W., Engbersen J.F.J., and Reinhoudt D.N. Crown ethers can enhance enzyme activity in organic solvents. Prog. Biotech. Biocatal. Non-Conventional Media 8 (1992) 691-695
5. Carpenter J.F., Crowe L.M., and Crowe J.H. Stabilization of phosphofructokinase with sugars during freeze-drying: characterization of enhanced protection in the presence of divalent cations. Biochem. Biophys. Act. 923 (1987) 109-115
6. Carpenter J.H., and Crowe J.H. The mechanism of cryoprotection of proteins by solutes. Crybiol. 25 (1988) 244-255
7. Carpenter J.F., and Crowe J.H. An infrared spectroscopic study of the interactions of carbohydrates with dried proteins. Biochem. 28 (1989) 3916-3922
8. Carpenter J.F., Prestrelski S.J., and Arakawa T. Separation of freezing- and drying-induced denaturation of lyophilized proteins using stress-specific stabilization. I. Enzyme activity and calorimetric studies. Arch. Biochem. Biophys. 303 (1993) 456-464
9. Carpenter J.F., Pikal M.J., Chang B.S., and Randolph T.W. Rational design of stable lyophilized protein formulations: some practical advice. Pharm. Res. 14 (1997) 969-975
10. Chefson A., and Auclair K. Progress towards easier use of P450 enzymes. Mol. Biosys. 2 (2006) 462-469
11. Chefson A., Zhao J., and Auclair K. Replacement of natural cofactors by selected hydrogen peroxide donors or organic peroxides results in improved activity for CYP3A4 and CYP2D6. Chem. Bio. Chem. 7 (2006) 916-919
12. Crowe J.H., Carpenter J.F., Crowe L.M., and Anchordoguy T.J. Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiol. 27 (1990) 219-231
13. D'Andrea G., Salucci M.L., and Avigliano L. Effects of lyoprotectants on ascorbate oxidase activity after freeze-drying and storage. Proc. Biochem. 31 (1996) 173-178
14. DePaz R.A., Dale D.A., Barnett C.C., Carpenter J.F., Gaertner A.L., and Randoplh T.W. Effects of drying methods and additives on the structure, function, and storage stability of substilisin: role of protein conformation and molecular mobility. Enz. Microb. Technol. 31 (2002) 765-774
15. Griebenow K., Laureano Y.D., Santos A.M., Clemente Y.M., Rodriguez L., Vidal M.W., and Barletta G. Improved enzyme activity and enantioselectivity in organic solvents by methyl-β-cyclodextrin. J. Am. Chem. Soc. 121 (1999) 8157-8163
16. Izutsu K., and Yoshioka S. Stabilization of protein pharmaceuticals in freeze-dried formulations. Drug Stab. 1 (1995) 11-21
17. Kreilgaard L., Frokjaer S., Flink J.M., Randolph T.W., and Carpenter J.F. Effects of additives on the stability of recombinant human factor XIII during freeze-drying and storage in the dried solid. Arch. Biochem. Biophys. 360 (1998) 121-134
18. Lee M.-Y., and Dordick J.S. Enzyme activation for nonaqueous media. Curr. Opin. Biotechnol. 13 (2002) 376-384
19. Liao Y.-H., Brown M.B., Nazir T., Quader A., and Martin G.P. Effects of sucrose and trehalose on the preservation of the native structure of spray-dried lysozyme. Pharma. Res. 19 (2002) 1847-1853
20. Liao Y.-H., Brown M.B., Quader A., and Martin G.P. Protective mechanism of stabilizing excipients against dehydration in the freeze-drying of proteins. Pharma. Res. 19 (2002) 1854-1861
21. Lippert K., and Galinski E.A. Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying. Appl. Microbiol. Biotechnol. 37 (1992) 61-65
22. Mine Y., Fukunaga K., Itoh K., Yoshimoto M., Nakao K., and Sugimura Y. Enhanced enzyme activity and enantioselectivity of lipases in organic solvents by crown ethers and cyclodextrins. J. Biosci. Bioeng. 95 (2003) 441-447
23. Prestrelski S.J., Tedeschi N., Arakawa T., and Carpenter J.F. Dehydration induced conformational transitions in proteins and their inhibition by stabilizers. Biophys. J. 65 (1993) 661-671
24. Prestrelski S.J., Tedeschi N., Arakawa T., and Carpenter J.F. Separation of freezing- and drying-induced denaturation of lyophilized proteins using stress-specific stabilization. II. Structural studies using infrared spectroscopy. Arch. Biochem. Biophys. 303 (1993) 465-473
25. Roy I., and Gupta M.N. Freeze-drying of proteins: some emerging concerns. Biotechnol. Appl. Biochem. 39 (2004) 165-177
26. +-ATPase from Kluyveromyces lactis during freeze-drying and rehydration. Cryobiol. 37 (1998) 131-138
27. Santos A.M., Montanez-Clemente I., Barlett G., and Griebnow K. Activation of serine protease subtilisin Carlsberg in organic solvents: combined effect of methyl-β-cyclodextrin and water. Biotech. Lett. 21 (1999) 1113-1118
28. Santos A.M., Vidal M., Pacheco Y., Frontera J., Baez C., Ornellas O., Barletta G., and Griebenow K. Effect of crown ethers on structure, stability, activity and enantioselectivity of subtilisin Carlsberg in organic solvents. Biotechnol. Bioeng. 74 (2001) 295-308
29. Souillac P.O., Costantino H.R., Middaugh C.R., and Rytting J.H. Investigation of protein/carbohydrate interactions in the dried state.1. Calorimetric studies. J. Pharma. Sci. 91 (2002) 206-216
30. Souillac P.O., Middaugh C.R., and Rytting J.H. Investigation of protein/carbohydrate interactions in the dried state. 2. Diffuse reflectance FTIR studies. Int. J. Pharma. 235 (2002) 207-218
31. Tanaka R., Takeda T., and Miyajima R. Cryoprotective effect of saccharides on denaturation of catalase during freeze-drying. Chem. Pharm. Biol. 31 (1991) 1091-1094
32. Townsend M.W., and DeLuca P.P. Use of lyoprotectants in the freeze-drying of a model protein, ribonuclease A. J. Parenter. Sci. Technol. 42 (2002) 190-199
33. Tremblay M., Cote S., and Voyer N. Enhanced activity of α-chymotrypsin in organic media using designed molecular staples. Tetrahedron 61 (2005) 6824-6828
34. Urlacher V.B., and Eiben S. Cytochrome P450 monooxygenases: perspectives for synthetic application. Trends Biotechnol. 24 (2006) 324-330
35. van Unen D.-J., Engbersen J.F.J., and Reinhoudt D.N. Why do crown ethers activate enzymes in organic solvents? Biotech. Bioeng. 77 (2002) 248-255
36. Ward K.R., Adams G.D.J., Alpar H.O., and Irwin W.J. Protection of the enzyme L-asparaginase during lyophilization - a molecular modeling approach to predict required level of lyoprotectants. Inter. J. Pharma. 187 (1999) 153-162