Effects of drying methods and additives on the structure, function, and storage stability of subtilisin: Role of protein conformation and molecular mobility

Enzyme and Microbial Technology

Volumn 31, Issue 6, 2002, Pages 765-774

  DePaz, Roberto A ^a   Dale, Douglas A ^b   Barnett, Christopher C ^b   Carpenter, John F ^c   Gaertner, Alfred L ^b   Randolph, Theodore W ^a  

^a UCB 450   (United States)

^b GENENCOR INTERNATIONAL INC   (United States)

^c UNIVERSITY OF COLORADO   (United States)

Author keywords

Detergents; Enzymes; Infrared spectroscopy; Lyophilization; Methionine oxidation; Spray coating; Storage stability; Subtilisin

Indexed keywords

AMINO ACIDS; CARBOHYDRATES; CONFORMATIONS; DEHYDRATION; DETERGENTS; HYDROGEN PEROXIDE; HYDROLYSIS; INFRARED SPECTROSCOPY; OXIDATION; POLYMERS;

CATALYTIC ACTIVITY;

ENZYMES;

DETERGENT; DEXTRAN; DISACCHARIDE; HYDROGEN PEROXIDE; MALTODEXTRIN; METHIONINE; POLYMER; SUBTILISIN; SUCROSE; TREHALOSE; WATER;

AQUEOUS SOLUTION; ARTICLE; CATALYSIS; CONTROLLED STUDY; DEHYDRATION; ENZYME CONFORMATION; ENZYME STABILITY; ENZYME STRUCTURE; FREEZE DRYING; HYDROGEN BOND; HYDROLYSIS; INFRARED SPECTROSCOPY; MOLECULAR DYNAMICS; NONHUMAN; OXIDATION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN SECONDARY STRUCTURE; REHYDRATION; SPRAY COATING; STORAGE; TECHNIQUE;

EID: 0036843324     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00173-4     Document Type: Article

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